| 2025 Challenger de Santiago | |
|---|---|
| Date | 10–16 March |
| Edition | 19th |
| Surface | Clay |
| Location | Santiago, Chile |
| 2024 Champions | |
| Singles | |
 Juan Pablo Varillas | |
| Doubles | |
 Fernando Romboli / Marcelo Zormann | |
The 2025 Challenger de Santiago was a professional tennis tournament played on clay courts. It was the 19th edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Santiago, Chile between 10 and 16 March 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| BRA | Thiago Monteiro | 105 | 1 |
 COL | Daniel Elahi Galán | 123 | 2 |
 CHI | Tomás Barrios Vera | 153 | 3 |
 ARG | Juan Pablo Ficovich | 169 | 4 |
 PAR | Daniel Vallejo | 195 | 5 |
| PER | Juan Pablo Varillas | 198 | 6 |
 BOL | Murkel Dellien | 199 | 7 |
| ARG | Andrea Collarini | 202 | 8 |
The following players received wildcards into the singles main draw:
Diego Dedura-Palomero Benjamín Torrealba Nicolás VillalónThe following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
vs. / vs. /