| 2025 Knoxville Challenger | |
|---|---|
| Date | 3–9 November |
| Edition | 21st |
| Surface | Hard (Indoor) |
| Location | Knoxville, United States |
| 2024 Champions | |
| Singles | |
| | |
| Doubles | |
| | |
The 2025 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the 21st edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Knoxville, United States between 3 and 9 November 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| James Trotter | 198 | 1 | |
| Jay Clarke | 203 | 2 | |
| Nicolás Mejía | 205 | 3 | |
| Johannus Monday | 212 | 4 | |
| Mitchell Krueger | 242 | 5 | |
| Daniil Glinka | 263 | 6 | |
| Alex Rybakov | 264 | 7 | |
| Andres Martin | 270 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw: