| 2025 Knoxville Challenger | |
|---|---|
| Date | 3–9 November |
| Edition | 21st |
| Surface | Hard (Indoor) |
| Location | Knoxville, United States |
| Champions | |
| Singles | |
 Mitchell Krueger | |
| Doubles | |
 Patrick Harper / Quinn Vandecasteele | |
The 2025 Knoxville Challenger was a professional tennis tournament played on indoor hard courts. It was the 21st edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Knoxville, United States between 3 and 9 November 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 JPN | James Trotter | 198 | 1 |
 GBR | Jay Clarke | 203 | 2 |
 COL | Nicolás Mejía | 205 | 3 |
| GBR | |||
| USA | Mitchell Krueger | 242 | 5 |
 EST | Daniil Glinka | 263 | 6 |
| USA | Alex Rybakov | 264 | 7 |
| USA | Andres Martin | 270 | 8 |
The following players received wildcards into the singles main draw:
Andrew Fenty Alexander Kotzen Dominique RollandThe following player received entry into the singles main draw through the Junior Accelerator programme:
Roh Ho-youngThe following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
Murphy Cassone 
Péter Makk Shunsuke Mitsui 
Francisco Rocha
Dhakshineswar Suresh 
Max Wiskandt The following player received entry as a lucky loser:
Mitchell Krueger def. Darwin Blanch 6–7(2–7), 6–4, 6–1. Patrick Harper / Quinn Vandecasteele def. Mitchell Krueger / 
Jody Maginley 6–7(6–8), 7–6(7–4), [12–10].