| 2025 Antofagasta Challenger | |
|---|---|
| Date | 29 September – 5 October |
| Edition | 3rd |
| Surface | Clay |
| Location | Antofagasta, Chile |
| 2024 Champions | |
| Singles | |
 Juan Manuel Cerúndolo | |
| Doubles | |
 Mateus Alves /  Matías Soto | |
The 2025 Antofagasta Challenger was a professional tennis tournament played on clay courts. It was the third edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Antofagasta, Chile between 29 September and 5 October 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 USA | Emilio Nava | 90 | 1 |
| CHI | Cristian Garín | 124 | 2 |
 BOL | Hugo Dellien | 126 | 3 |
| CHI | Tomás Barrios Vera | 133 | 4 |
 ITA | Francesco Maestrelli | 154 | 5 |
 LBN | Hady Habib | 172 | 6 |
 AUT | Lukas Neumayer | 175 | 7 |
| ARG | Alex Barrena | 188 | 8 |
1 Rankings are as of 22 September 2025. [1]
The following players received wildcards into the singles main draw:
Facundo Bagnis Daniel Antonio Núñez Benjamín TorrealbaThe following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
vs. / vs. /