| 2025 AON Open Challenger | |
|---|---|
| Date | 1–7 September |
| Edition | 21st |
| Surface | Clay |
| Location | Genoa, Italy |
| Champions | |
| Singles | |
 Luciano Darderi | |
| Doubles | |
 Mick Veldheer /  Szymon Walków | |
The 2025 AON Open Challenger was a professional tennis tournament played on clay courts. It was the 21st edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Genoa, Italy between 1 and 7 September 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
| ITA | Luciano Darderi | 34 | 1 |
 ESP | Pedro Martínez | 66 | 2 |
 CRO | |||
 DEN | |||
 ARG | Thiago Agustín Tirante | 115 | 5 |
| ITA | Francesco Passaro | 120 | 6 |
 FRA | Pierre-Hugues Herbert | 139 | 7 |
| ITA | Andrea Pellegrino | 141 | 8 |
The following players received wildcards into the singles main draw:
Carlo Alberto Caniato Lorenzo Carboni Luciano Darderi The following player received entry into the singles main draw using a protected ranking:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
Gianluca Cadenasso Tommaso Compagnucci Enrico Dalla Valle 
Tom Gentzsch Gabriele Pennaforti Andrea PicchioneThe following players received entry as lucky losers:
Luciano Darderi def. Andrea Pellegrino 6–1, 6–3. Mick Veldheer / Szymon Walków def. Gianluca Cadenasso / Lorenzo Carboni 3–6, 6–4, [10–7].