| 2025 Cali Open | |
|---|---|
| Date | 6 – 12 October |
| Edition | 4th |
| Surface | Clay |
| Location | Cali, Colombia |
| 2024 Champions | |
| Singles | |
 Juan Pablo Ficovich | |
| Doubles | |
 Juan Carlos Aguilar /  Conner Huertas del Pino | |
The 2025 Cali Open was a professional tennis tournament played on clay courts. It was the fourth edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Cali, Colombia between 6 and 12 October 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 CHI | Tomás Barrios Vera | 137 | 1 |
| ARG | Juan Pablo Ficovich | 147 | 2 |
 BRA | Thiago Seyboth Wild | 162 | 3 |
 LBN | Hady Habib | 175 | 4 |
 AUT | Lukas Neumayer | 177 | 5 |
 COL | Nicolás Mejía | 198 | 6 |
 MEX | Rodrigo Pacheco Méndez | 200 | 7 |
 ECU | Álvaro Guillén Meza | 205 | 8 |
The following players received wildcards into the singles main draw:
Samuel Heredia Samuel Alejandro Linde Palacios Miguel TobónThe following player received entry into the singles main draw as an alternate:
The following players received entry from the qualifying draw:
vs. / vs. / 