| 2025 Napoli Tennis Cup | |
|---|---|
| Date | 24–30 March |
| Edition | 23rd |
| Surface | Clay / outdoor |
| Location | Naples, Italy |
| Venue | Tennis Club Napoli |
| Champions | |
| Singles | |
 Vít Kopřiva | |
| Doubles | |
 Alexander Erler /  Constantin Frantzen | |
The 2025 Napoli Tennis Cup was a professional men's tennis tournament played on outdoor clay courts. It was the 23rd edition of the tournament, and part of the 2025 ATP Challenger Tour. It took place at the Tennis Club Napoli in Naples, Italy from 24 to 30 March 2025.
Vít Kopřiva def. 
Luciano Darderi 3–6, 6–3, 7–6(7–4). Alexander Erler / Constantin Frantzen def. 
Geoffrey Blancaneaux / Albano Olivetti 6–4, 6–4.| Country | Player | Rank1 | Seed |
|---|---|---|---|
| ITA | Luciano Darderi | 61 | 1 |
 BEL | Raphaël Collignon | 91 | 2 |
| ITA | Francesco Passaro | 94 | 3 |
 BRA | Thiago Seyboth Wild | 96 | 4 |
| ITA | Fabio Fognini | 97 | 5 |
 USA | Tristan Boyer | 111 | 6 |
 CRO | Borna Ćorić | 112 | 7 |
 TPE | Tseng Chun-hsin | 115 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry into the singles main draw as alternates:
The following players received entry from the qualifying draw:
Franco Agamenone Hynek Bartoň Giovanni Fonio Borna Gojo Andrea Pellegrino 
Elias Ymer 