| 2025 Winston-Salem Challenger | |
|---|---|
| Date | 8–14 September |
| Edition | 1st |
| Surface | Hard |
| Location | Winston-Salem, North Carolina, United States |
| Champions | |
| Singles | |
 Jack Pinnington Jones | |
| Doubles | |
 Andrew Fenty / Noah Schachter | |
The 2025 Winston-Salem Challenger was a professional tennis tournament played on hardcourts. It was the first edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Winston-Salem, North Carolina, United States between 8 and 14 September 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 ARG | Juan Pablo Ficovich | 129 | 1 |
| USA | Murphy Cassone | 180 | 2 |
| USA | Mitchell Krueger | 186 | 3 |
| GBR | Jack Pinnington Jones | 222 | 4 |
| GBR | Johannus Monday | 232 | 5 |
| USA | Garrett Johns | 305 | 6 |
| USA | Alex Rybakov | 314 | 7 |
| USA | Patrick Maloney | 324 | 8 |
1 Rankings are as of 25 August 2025. [1]
The following players received wildcards into the singles main draw:
Patrick Maloney Luca Pow Emon van Loben SelsThe following players received entry into the singles main draw using protected rankings:
The following player received entry into the singles main draw through the College Accelerator programme:
Aidan KimThe following players received entry from the qualifying draw:
Gavin Goode Daniel Milavsky
James Trotter Quinn Vandecasteele Cooper Williams 
Edward Winter Jack Pinnington Jones def. Trevor Svajda 6–2, 6–2. Andrew Fenty / Noah Schachter def. 
Juan José Bianchi / Daniel Milavsky 6–4, 6–4.