| 2025 Challenger Ciudad de Guayaquil | |
|---|---|
| Date | 17 – 23 November |
| Edition | 21st |
| Surface | Clay |
| Location | Guayaquil, Ecuador |
| 2024 Champions | |
| Singles | |
 Federico Agustín Gómez | |
| Doubles | |
 Karol Drzewiecki / Piotr Matuszewski | |
The 2025 Challenger Ciudad de Guayaquil was a professional tennis tournament played on clay courts. It was the 21st edition of the tournament which was part of the 2025 ATP Challenger Tour. It took place in Guayaquil, Ecuador between 17 and 23 November 2025.
| Country | Player | Rank1 | Seed |
|---|---|---|---|
 CHI | |||
 PAR | Daniel Vallejo | 169 | 2 |
 LBN | Hady Habib | 173 | 3 |
 ECU | Álvaro Guillén Meza | 200 | 4 |
 PER | Gonzalo Bueno | 206 | 5 |
 MEX | Rodrigo Pacheco Méndez | 209 | 6 |
 BOL | Murkel Dellien | 262 | 7 |
| ARG | Federico Agustín Gómez | 270 | 8 |
| ECU | Andrés Andrade | 283 | 9 |
The following players received wildcards into the singles main draw:
Emilio Camacho Mario André Galarraga Lucas YúnezThe following players received entry into the singles main draw as alternates:
Joaquín Aguilar Cardozo
Ryan Dickerson Juan EstévezThe following players received entry from the qualifying draw:
vs. / vs. /