Lacto-N-biosidase

Lacto-N-biosidase
Identifiers
EC no.	3.2.1.140
CAS no.	146359-52-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Lacto-N-biosidase (EC 3.2.1.140) is an enzyme with systematic name oligosaccharide lacto-N-biosylhydrolase. ^{[1] [2]} This enzyme catalyses the following chemical reaction

(1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H₂O ${\displaystyle \rightleftharpoons }$ beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc

(2) lacto-N-tetraose + H₂O ${\displaystyle \rightleftharpoons }$ lacto-N-biose + lactose

The enzyme from Streptomyces specifically hydrolyses the terminal lacto-N-biosyl residue (beta-D-Gal-(1->3)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).

References

1. Sano M, Hayakawa K, Kato I (September 1992). "An enzyme releasing lacto-N-biose from oligosaccharides". Proceedings of the National Academy of Sciences of the United States of America. 89 (18): 8512–8516. Bibcode:1992PNAS...89.8512S. doi: 10.1073/pnas.89.18.8512 . PMC   49950 . PMID   1528855.
2. Sano M, Hayakawa K, Kato I (September 1993). "Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain". The Journal of Biological Chemistry. 268 (25): 18560–18566. doi: 10.1016/S0021-9258(17)46664-6 . PMID   7689556.

External links

• Lacto-N-biosidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)