List of restriction enzyme cutting sites: G–K

Last updated

Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
NA, C, G or T
MA or C
RA or G
WA or T
YC or T
SC or G
KG or T
HA, C or T
BC, G or T
VA, C or G
DA, G or T

This article contains a list of the most studied restriction enzymes whose names start with G to K inclusive. It contains approximately 90 enzymes.

Contents

The following information is given:

as in this not listed enzyme: EcoR70I 

Whole list navigation

Restriction enzymes

G

EnzymePDB codeSource Recognition sequence Cut Isoschizomers
GalI Gluconobacter albidus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
GceI Gluconobacter cerinus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
GceGLI Gluconobacter cerinus 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
GdiI Gluconobacter dioxyacetonicus 5' AGGCCT
3' TCCGGA 		5' ---AGG   CCT--- 3'
3' ---TCC   GGA--- 5' 		AatI, AspMI, Eco147I, PceI, SarI, Sru30DI, SseBI, SteI, StuI
GdiII Gluconobacter dioxyacetonicus 5' CGGCCR
3' GCCGGY 		5' ---C   GGCCR--- 3'
3' ---GCCGG   Y--- 5'
GstI Geobacillus stearothermophilus 5' GGATCC
3' CCTAGG 		5' ---G   GATCC--- 3'
3' ---CCTAG   G--- 5'
GsuI Gluconobacter suboxydans H-15T 5' CTGGAG
3' GACCTC 		5' ---CTGGAGN12NNNN   --- 3'
3' ---GACCTCN12NN   NN--- 5'

H

EnzymePDB codeSource Recognition sequence Cut Isoschizomers
HacI Halococcus acetoinfaciens 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bme12I, Bsp67I, BstENII, CcyI, FnuEI, MgoI, NphI, Sau3AI
HaeI Haemophilus aegyptius 5' WGGCCW
3' WCCGGW 		5' ---WGG   CCW--- 3'
3' ---WCC   GGW--- 5'
HaeII Haemophilus aegypticus 5' RGCGCY
3' YCGCGR 		5' ---RGCGC   Y--- 3'
3' ---Y   CGCGR--- 5'
AccB2I,  BfoI, Bme142I,
Bsp143II, BstH2I, LpnI
HaeIII Haemophilus aegypticus 5' GGCC
3' CCGG 		5' ---GG   CC--- 3'
3' ---CC   GG--- 5' 		BsuRI
HaeIV Haemophilus aegyptius 5' GAYN5RTC
3' CTRN5YAG 		5' ---GAYN5RTCN8NNNNNN   --- 3'
3' ---CTRN5YAGN8N   NNNNN--- 5'
HalI Hafnia alvei B6 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
HalII Hafnia alvei B6 5' CTGCAG
3' GACGTC 		5' ---CTGCA   G--- 3'
3' ---G   ACGTC--- 5' 		AjoI, AliAJI, Bsp63I, CfrA4I, CfuII, CstI, PstI, SflI, Srl5DI, Sst12I
HapII Haemophilus aphrophilus 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
HgaI [7] Haemophilus gallinarum 5' GACGC
3' CTGCG 		5' ---GACGCN4N   NNNNN--- 3'
3' ---CTGCGN4NNNNNN   --- 5'
HgiI Herpetosiphon giganteus 3303 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AsuIII, BbiII, BstACI, HgiGI, HgiHII, Hin1I, PamII
HgiAI Herpetosiphon giganteus HP1023 5' GWGCWC
3' CWCGWG 		5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
Alw21I, AspHI, Bsh45I, BsiHKAI,
 Bsm6I, HpyF46II, MspV281I
HgiBI Herpetosiphon giganteus Hpg5 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BthAI, DsaIV, ErpI, FspMSI, HgiEI, Psp03I, VpaK11AI
HgiCI Herpetosiphon giganteus Hpg9 5' GGYRCC
3' CCRYGG 		5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5' 		AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiHI, MspB4I, PfaAI
HgiCII Herpetosiphon giganteus Hpg9 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme18I, BthAI, DsaIV, FspMSI, HgiHIII, Psp03I, VpaK11AI
HgiCIII Herpetosiphon giganteus Hpg9 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
HgiDI Herpetosiphon giganteus Hpa2 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AsuIII, BbiII, BstACI, HgiGI, HgiI, Hin1I, PamII
HgiDII Herpetosiphon giganteus Hpa2 5' GTCGAC
3' CAGCTG 		5' ---G   TCGAC--- 3'
3' ---CAGCT   G--- 5'
HgiEI Herpetosiphon giganteus Hpg24 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, BthAI, DsaIV, ErpI, FspMSI, HgiHIII, VpaK11AI
HgiGI Herpetosiphon giganteus Hpa1 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AosII, BbiII, BstACI, HgiI, HgiGI, Msp17I, PamII
HgiHI Herpetosiphon giganteus HP1049 5' GGYRCC
3' CCRYGG 		5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5' 		AccB1I, BanI, BspT107I, BshNI, Eco64I, HgiCI, MspB4I, PfaAI
HgiHII Herpetosiphon giganteus HP1049 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AosII, AsuIII, BsaHI, BstACI, HgiGI, HgiHII, Msp17I, PamII
HgiHIII Herpetosiphon giganteus HP1049 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, DsaIV, ErpI, HgiBI, Psp03I, VpaK11AI, VpaK11BI
HgiJI Herpetosiphon giganteus HFS101 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11AI
HgiJII Herpetosiphon giganteus HFS101 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
HgiS22I Herpetosiphon giganteus 5' CCSGG
3' GGSCC 		5' ---CC   SGG--- 3'
3' ---GGS   CC--- 5' 		AhaI, AseII, AsuC2I, BcnI, CauII, Eco1831I, EcoHI, Kpn49kII
HhaI Haemophilus haemolyticus 5' GCGC
3' CGCG 		5' ---GCG   C--- 3'
3' ---C   GCG--- 5' 		AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, Hin6I, HinP1I, SciNI
HhaII Haemophilus haemolyticus 5' GANTC
3' CTNAG 		5' ---G   ANTC--- 3'
3' ---CTNA   G--- 5'
Hin1I Haemophilus influenzae RFL1 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AhaII, AosII, AstWI, AsuIII, HgiI, HgiDI, HgiDI, Hsp92I
Hin1II Haemophilus influenzae RFL1 5' CATG
3' GTAC 		5' ---CATG   --- 3'
3' ---   GTAC--- 5'
Hin2I Haemophilus influenzae RFL2 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
Hin4I Haemophilus influenzae RFL4 5' GAYN5VTC
3' CTRN5BAG 		5' ---GAYN5VTCN7NNNNNN   --- 3'
3' ---CTRN5BAGN7N   NNNNN--- 5'
Hin6I Haemophilus influenzae RFL6 5' GCGC
3' CGCG 		5' ---G   CGC--- 3'
3' ---CGC   G--- 5' 		AspLEI, BspLAI, BstHHI, CfoI, FnuDIII, HhaI, HsoI, HspAI, SciNI
HinJCI Haemophilus influenzae JC9 5' GTYRAC
3' CARYTG 		5' ---GTY   RAC--- 3'
3' ---CAR   YTG--- 5'
HinP1I 2FL3 Search PDBe Haemophilus influenzae P1 5' GCGC
3' CGCG 		5' ---G   CGC--- 3'
3' ---CGC   G--- 5' 		BspLAI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HsoI, HspAI, SciNI
HincII 2AUD Search PDBe Haemophilus influenzae Rc 5' GTYRAC
3' CARYTG 		5' ---GTY   RAC--- 3'
3' ---CAR   YTG--- 5'
HindII Haemophilus influenzae Rd 5' GTYRAC
3' CARYTG 		5' ---GTY   RAC--- 3'
3' ---CAR   YTG--- 5'
HindIII 2E52, Search PDBe Haemophilus influenzae Rd 5' AAGCTT
3' TTCGAA 		5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'
HinfI Haemophilus influenzae Rf 5' GANTC
3' CTNAG 		5' ---G   ANTC--- 3'
3' ---CTNA   G--- 5'
HjaI Hyphomonas jannaschiana 5' GATATC
3' CTATAG 		5' ---GAT   ATC--- 3'
3' ---CTA   TAG--- 5'
HpaI Haemophilus parainfluenzae 5' GTTAAC
3' CAATTG 		5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
HpaII Haemophilus parainfluenzae 5' CCGG
3' GGCC 		5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
HphI Haemophilus parahaemolyticus 5' GGTGA
3' CCACT 		5' ---GGTGAN6NN   --- 3'
3' ---CCACTN6N   N--- 5' 		AsuHPI, SspD5I
Hpy8I Helicobacter pylori A 8-5 5' GTNNAC
3' CANNTG 		5' ---GTN   NAC--- 3'
3' ---CAN   NTG--- 5'
Hpy51I Helicobacter pylori 51 5' GTSAC
3' CASTG 		5' ---   GTSAC--- 3'
3' ---CASTG   --- 5'
Hpy99I 3GOX Helicobacter pylori J99 5' CGWCG
3' GCWGC 		5' ---CGWCG   --- 3'
3' ---   GCWGC--- 5'
Hpy178III Helicobacter pylori J178 5' TCNNGA
3' AGNNCT 		5' ---TC   NNGA--- 3'
3' ---AGNN   CT--- 5'
Hpy188I Helicobacter pylori J188 5' TCNGA
3' AGNCT 		5' ---TCN   GA--- 3'
3' ---AG   NCT--- 5'
Hpy188III Helicobacter pylori J188 5' TCNNGA
3' AGNNCT 		5' ---TC   NNGA--- 3'
3' ---AGNN   CT--- 5'
HpyAV Helicobacter pylori 26695 5' CCTTC
3' GGAAG 		5' ---CCTTCN4NN   --- 3'
3' ---GGAAGN4N   N--- 5'
HpyBI Helicobacter pylori Roberts 5' GTAC
3' CATG 		5' ---GT   AC--- 3'
3' ---CA   TG--- 5' 		AfaI, Csp6I, CviQI, CviRII, PabI, PlaAII, RsaI, RsaNI
HpyBII Helicobacter pylori Roberts 5' GTNNAC
3' CANNTG 		5' ---GTN   NAC--- 3'
3' ---CAN   NTG--- 5'
HpyCI Helicobacter pylori 5' GATATC
3' CTATAG 		5' ---GAT   ATC--- 3'
3' ---CTA   TAG--- 5'
HpyC1I Helicobacter pylori 5' CCATC
3' GGTAG 		5' ---CCATCNNNN   N--- 3'
3' ---GGTAGNNNNN   --- 5'
HpyCH4I Helicobacter pylori CH4 5' CATG
3' GTAC 		5' ---CATG   --- 3'
3' ---   GTAC--- 5'
HpyCH4III Helicobacter pylori CH4 5' ACNGT
3' TGNCA 		5' ---ACN   GT--- 3'
3' ---TG   NCA--- 5'
HpyCH4IV Helicobacter pylori CH4 5' ACGT
3' TGCA 		5' ---A   CGT--- 3'
3' ---TGC   A--- 5'
HpyCH4V Helicobacter pylori CH4 5' TGCA
3' ACGT 		5' ---TG   CA--- 3'
3' ---AC   GT--- 5'
HpyF10VI Helicobacter pylori RFL10 5' GCN7GC
3' CGN7CG 		5' ---GCNNNNN   NNGC--- 3'
3' ---CGNN   NNNNNCG--- 5'
HpyF44III Helicobacter pylori RFL44 5' TGCA
3' ACGT 		5' ---TG   CA--- 3'
3' ---AC   GT--- 5'
HsoI Haemophilus somnus 2336 5' GCGC
3' CGCG 		5' ---G   CGC--- 3'
3' ---CGC   G--- 5' 		AspLEI, BstHHI, CfoI, FnuDIII, Hin6I, HinP1I, HspAI, SciNI
Hsp92I Haemophilus sp. 92 5' GRCGYC
3' CYGCRG 		5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5' 		AcyI, AhaII, AosII, AstWI, HgiI, HgiDI, HgiHII, Hin1I, Hsp92I
Hsp92II Haemophilus sp. 92 5' CATG
3' GTAC 		5' ---CATG   --- 3'
3' ---   GTAC--- 5'
HspAI Haemophilus sp. A 5' GCGC
3' CGCG 		5' ---G   CGC--- 3'
3' ---CGC   G--- 5' 		AspLEI, BspLAI, BstHHI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, SciNI
HsuI Haemophilus suis 5' AAGCTT
3' TTCGAA 		5' ---A   AGCTT--- 3'
3' ---TTCGA   A--- 5'

I

EnzymePDB codeSource Recognition sequence Cut Isoschizomers
ItaI Ilyobcater tartaricus 5' GCNGC
3' CGNCG 		5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'

K

EnzymePDB codeSource Recognition sequence Cut Isoschizomers
KasI Kluyvera ascorbata 5' GGCGCC
3' CCGCGG 		5' ---G   GCGCC--- 3'
3' ---CCGCG   G--- 5'
Kaz48kI Klebsiella azeanae 5' RGGNCCY
3' YCCNGGR 		5' ---RGGNC   CY--- 3'
3' ---YC   CNGGR--- 5'
KoxII Klebsiella oxytoca 5' GRGCYC
3' CYCGRG 		5' ---GRGCY   C--- 3'
3' ---C   YCGRG--- 5'
KpnI [8] Klebsiella pneumoniae OK8 5' GGTACC
3' CCATGG 		5' ---GGTAC   C--- 3'
3' ---C   CATGG--- 5' 		Acc65I, AhaB8I, Asp718I, SthI
Kpn2I Klebsiella pneumoniae RFL2 5' TCCGGA
3' AGGCCT 		5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5' 		Aor13HI, BbvAIII, BseAI, BsiMI, BspEI, BspMII, CauB3I, MroI
Kpn378I Klebsiella pneumoniae 378 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Kpn2kI Klebsiella pneumoniae 2k 5' CCNGG
3' GGNCC 		5' ---   CCNGG--- 3'
3' ---GGNCC   --- 5'
Kpn49kI Klebsiella pneumoniae 49k 5' GAATTC
3' CTTAAG 		5' ---G   AATTC--- 3'
3' ---CTTAA   G--- 5'
Kpn49kII Klebsiella pneumoniae 49k 5' CCSGG
3' GGSCC 		5' ---   CCSGG--- 3'
3' ---GGSCC   --- 5' 		AhaI, AsuC2I, BcnI, CauII, EcoHI, HgiS22I, Mgl14481I, NciI
KspI Kluyvera sp. 5' CCGCGG
3' GGCGCC 		5' ---CCGC   GG--- 3'
3' ---GG   CGCC--- 5'
Ksp22I Kurthia sp. 22 5' TGATCA
3' ACTAGT 		5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
AbaI, BclI, BsiQI,
BspXII, BstT7I, FbaI,  ParI 
Ksp632I Kluyvera sp. 632 5' CTCTTC
3' GAGAAG 		5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
KspAI Kurthia sp. N88 5' GTTAAC
3' CAATTG 		5' ---GTT   AAC--- 3'
3' ---CAA   TTG--- 5'
Kzo9I Kurthia zopfii 9 5' GATC
3' CTAG 		5' ---   GATC--- 3'
3' ---CTAG   --- 5' 		Bce243I, Bsp105I, BspFI, BstMBI, CpfI, LlaAI, NdeII, Sth368I
Kzo49I Kurthia zopfii 49 5' GGWCC
3' CCWGG 		5' ---G   GWCC--- 3'
3' ---CCWG   G--- 5' 		Bme216I, CauI, DsaIV, FdiI, HgiBI, HgiHIII, Psp03I, VpaK11AI

Notes

  1. ↑ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID   4588280.
  2. ↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC   152790 . PMID   12654995.
  3. ↑ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN   0-7167-4684-0.
  4. ↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID   4124164.
  5. ↑ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID   2172084.
  6. ↑ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC   55916 . PMID   11557805.
  7. ↑ R.J Roberts, 1988, Nucleic Acids Res. 16(suppl):271 From p.213 Molecular Cell Biology 4th Edition by Lodish, Berk, Zipursky, Matsudaira, Baltimore and Darnell.
  8. ↑ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN   0-7167-4366-3.

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A restriction enzyme, restriction endonuclease, REase, ENase orrestrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone of the DNA double helix.

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<i>Eco</i>RV Restriction enzyme

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<span class="mw-page-title-main">Homing endonuclease</span>

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<i>Eco</i>RI Restriction enzyme

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