Website | http://www.arabidopsis.info |
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The Nottingham Arabidopsis Stock Centre (NASC) provides seed and information resources to the International Arabidopsis Genome Project and the wider research community. It is based in the School of Biosciences at the University of Nottingham's Sutton Bonington Campus, in the English county of Nottinghamshire.
It holds more than 800,000 different stocks of seed representing nearly a million genotypes and provided a Genechip service from 2002-2013. Newly generated research stocks, mutants or lines of Arabidopsis thaliana are donated as samples to NASC where they are maintained and thus are made available to scientists worldwide.
Established in 1990 [1] [2] as part of the Plant Molecular Biology initiative of the Agricultural and Food Research Council (AFRC), the Centre is currently funded by the Biotechnology and Biological Sciences Research Council (BBSRC) and the University of Nottingham.
The Stock Centre was founded by Dr Bernard Mulligan; Directed from 1991 to 1999 by Dr Mary Anderson and from 1999–present by Prof. Sean Tobias May.
NASC's activities are coordinated with those of the Arabidopsis Biological Resource Center, (ABRC) based at Ohio State University, USA. This facilitates a unified and efficient service for the research community.
The stock centres have a distribution agreement. NASC distributes to Europe and ABRC distributes to the Americas. Laboratories in other locations may establish their primary affiliation with either centre.
When NASC started in 1990 it inherited hundreds of stocks from the Arabidopsis Information Service (AIS) - Started by Robbelen in 1965 and continued by Burger (1971), Kranz (1978), and Kranz and Kirchheim (1981, 1987), the AIS donated its complement of stocks listed in the 24th edition of the AIS stock book. [3] Stock numbers held at NASC/ABRC in the 1990s then increased steadily by many hundreds mainly due to new technologies of mass transformation (e.g. seed transformation by Ken Feldmann, University of Arizona); but also due to many new mutagenesis programs encouraged by the development of Arabidopsis as a major model organism accompanied by generous donations of seed from many international members of the community.
In 1999 NASC received a large influx of thousands of stocks generated through T-DNA transformation and donated by Pelletier and Bechtold from INRA. This was the first of many very large populations that came out of the community and which took the complement of stocks held by the stock centres into the hundreds of thousands. The largest (most populous) seed donations have been the GABI-kat lines from Germany via Bernd Weisshaar; [4] and the SALK lines from Joe Ecker. [5] Between them, these two populations account for more than half of all stocks held by the stock centres today.
Arabidopsis (rockcress) is a genus in the family Brassicaceae. They are small flowering plants related to cabbage and mustard. This genus is of great interest since it contains thale cress, one of the model organisms used for studying plant biology and the first plant to have its entire genome sequenced. Changes in thale cress are easily observed, making it a very useful model.
Arabidopsis thaliana, the thale cress, mouse-ear cress or arabidopsis, is a small plant from the mustard family (Brassicaceae), native to Eurasia and Africa. Commonly found along the shoulders of roads and in disturbed land, it is generally considered a weed.
Vernalization is the induction of a plant's flowering process by exposure to the prolonged cold of winter, or by an artificial equivalent. After vernalization, plants have acquired the ability to flower, but they may require additional seasonal cues or weeks of growth before they will actually do so. The term is sometimes used to refer to the need of herbal (non-woody) plants for a period of cold dormancy in order to produce new shoots and leaves, but this usage is discouraged.
The transfer DNA is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes . The T-DNA is transferred from bacterium into the host plant's nuclear DNA genome. The capability of this specialized tumor-inducing (Ti) plasmid is attributed to two essential regions required for DNA transfer to the host cell. The T-DNA is bordered by 25-base-pair repeats on each end. Transfer is initiated at the right border and terminated at the left border and requires the vir genes of the Ti plasmid.
Hydrotropism is a plant's growth response in which the direction of growth is determined by a stimulus or gradient in water concentration. A common example is a plant root growing in humid air bending toward a higher relative humidity level.
The ABC model of flower development is a scientific model of the process by which flowering plants produce a pattern of gene expression in meristems that leads to the appearance of an organ oriented towards sexual reproduction, a flower. There are three physiological developments that must occur in order for this to take place: firstly, the plant must pass from sexual immaturity into a sexually mature state ; secondly, the transformation of the apical meristem's function from a vegetative meristem into a floral meristem or inflorescence; and finally the growth of the flower's individual organs. The latter phase has been modelled using the ABC model, which aims to describe the biological basis of the process from the perspective of molecular and developmental genetics.
The MADS box is a conserved sequence motif. The genes which contain this motif are called the MADS-box gene family. The MADS box encodes the DNA-binding MADS domain. The MADS domain binds to DNA sequences of high similarity to the motif CC[A/T]6GG termed the CArG-box. MADS-domain proteins are generally transcription factors. The length of the MADS-box reported by various researchers varies somewhat, but typical lengths are in the range of 168 to 180 base pairs, i.e. the encoded MADS domain has a length of 56 to 60 amino acids. There is evidence that the MADS domain evolved from a sequence stretch of a type II topoisomerase in a common ancestor of all extant eukaryotes.
Evolutionary developmental biology (evo-devo) is the study of developmental programs and patterns from an evolutionary perspective. It seeks to understand the various influences shaping the form and nature of life on the planet. Evo-devo arose as a separate branch of science rather recently. An early sign of this occurred in 1999.
GAI or Gibberellic-Acid Insensitive is a gene in Arabidopsis thaliana which is involved in regulation of plant growth. GAI represses the pathway of gibberellin-sensitive plant growth. It does this by way of its conserved DELLA motif.
Dame Caroline Dean is a British plant scientist working at the John Innes Centre. She is focused on understanding the molecular controls used by plants to seasonally judge when to flower. She is specifically interested in vernalisation — the acceleration of flowering in plants by exposure to periods of prolonged cold. She has also been on the Life Sciences jury for the Infosys Prize from 2018.
Plant genetics is the study of genes, genetic variation, and heredity specifically in plants. It is generally considered a field of biology and botany, but intersects frequently with many other life sciences and is strongly linked with the study of information systems. Plant genetics is similar in many ways to animal genetics but differs in a few key areas.
The Arabidopsis Information Resource (TAIR) is a community resource and online model organism database of genetic and molecular biology data for the model plant Arabidopsis thaliana, commonly known as mouse-ear cress.
The Arabidopsis Biological Resource Center (ABRC) was established at Ohio State University in September, 1991. Primary support for the ABRC is provided by a National Science Foundation grant. The mission of the ABRC is to acquire, preserve and distribute seed and DNA resources that are useful to the Arabidopsis research community.
HOTHEAD is an Arabidopsis thaliana gene that encodes a flavin adenine dinucleotide-containing oxidoreductase. This gene has a role in the creation of the carpel during the formation of flowers through the fusion of epidermal cells. Observations of reversion of the hothead phenotype and genotype led to the suggestion that the plants were able to "remember" the sequences of genes present in their ancestors, possibly through a cache of complementary RNA. This report attracted broad attention, and alternative explanations were suggested. Later research suggested that the supposed reversion phenomenon was due to the plants having a pronounced bias towards outcrossing, rather than self-fertilizing at high rates, as is typical for A. thaliana.
Gerd Jürgens is a plant developmental biologist and emeritus Director of the Cell Biology Department at the Max Planck Institute for Developmental Biology and Head of the Center for Plant Molecular Biology (ZMBP) at the Eberhard-Karls Universität Tübingen. He has published extensively in leading journals, including eight papers in the journal Nature as well as various articles in the journals Cell, Science, Journal of Cell Biology and The Plant Journal.
In biology, phototropism is the growth of an organism in response to a light stimulus. Phototropism is most often observed in plants, but can also occur in other organisms such as fungi. The cells on the plant that are farthest from the light contain a hormone called auxin that reacts when phototropism occurs. This causes the plant to have elongated cells on the furthest side from the light. Phototropism is one of the many plant tropisms, or movements, which respond to external stimuli. Growth towards a light source is called positive phototropism, while growth away from light is called negative phototropism. Negative phototropism is not to be confused with skototropism, which is defined as the growth towards darkness, whereas negative phototropism can refer to either the growth away from a light source or towards the darkness. Most plant shoots exhibit positive phototropism, and rearrange their chloroplasts in the leaves to maximize photosynthetic energy and promote growth. Some vine shoot tips exhibit negative phototropism, which allows them to grow towards dark, solid objects and climb them. The combination of phototropism and gravitropism allow plants to grow in the correct direction.
LUX or Phytoclock1 (PCL1) is a gene that codes for LUX ARRHYTHMO, a protein necessary for circadian rhythms in Arabidopsis thaliana. LUX protein associates with Early Flowering 3 (ELF3) and Early Flowering 4 (ELF4) to form the Evening Complex (EC), a core component of the Arabidopsis repressilator model of the plant circadian clock. The LUX protein functions as a transcription factor that negatively regulates Pseudo-Response Regulator 9 (PRR9), a core gene of the Midday Complex, another component of the Arabidopsis repressilator model. LUX is also associated with circadian control of hypocotyl growth factor genes PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and PHYTOCHROME INTERACTING FACTOR 5 (PIF5).
Arabidopsis thaliana is a first class model organism and the single most important species for fundamental research in plant molecular genetics.
Pseudo-response regulator (PRR) refers to a group of genes that regulate the circadian oscillator in plants. There are four primary PRR proteins that perform the majority of interactions with other proteins within the circadian oscillator, and another (PRR3) that has limited function. These genes are all paralogs of each other, and all repress the transcription of Circadian Clock Associated 1 (CCA1) and Late Elongated Hypocotyl (LHY) at various times throughout the day. The expression of PRR9, PRR7, PRR5 and TOC1/PRR1 peak around morning, mid-day, afternoon and evening, respectively. As a group, these genes are one part of the three-part repressilator system that governs the biological clock in plants.
Georges Pelletier is a French agricultural engineer and Doctor of Science. He spent his career at the National Institute of Agricultural Research (INRA) in the Department of Plant Genetics and Improvement. He headed the Unit of the INRA Versailles Centre from 1991 to 1999, chaired from 2001 to 2010, the Operational Management Board of the Group of Scientific Interest in Plant Genomics Genoplant, and from 2010 to 2013 was appointed to the French Agency for Research (ANR) scientific manager of the Biotechnologies and Bio-resources programme for "Investments for the Future". He was a member of the Biomolecular Engineering Commission and the Scientific Council of the Genopoles. Member of the French Academy of sciences (2004) and the Academy of Agriculture (2004), he was awarded the Lauriers d'excellence de INRA (2006).
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