ORC6

ORC6
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3M03
Identifiers
Aliases	ORC6 , ORC6L, origin recognition complex subunit 6
External IDs	OMIM: 607213; MGI: 1929285; HomoloGene: 8635; GeneCards: ORC6; OMA:ORC6 - orthologs
Gene location (Human) Chr. Chromosome 16 (human) [1] Band 16q11.2 Start 46,689,643 bp [1] End 46,698,394 bp [1]
Gene location (Mouse) Chr. Chromosome 8 (mouse) [2] Band 8|8 C3 Start 86,026,261 bp [2] End 86,034,907 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in oocyte endothelial cell secondary oocyte epithelium of colon right testis ventricular zone left testis gonad embryo testicle Top expressed in zygote genital tubercle secondary oocyte tail of embryo epiblast mandibular prominence otic placode maxillary prominence primary oocyte embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding DNA replication origin binding Cellular component nucleus membrane nucleoplasm origin recognition complex fibrillar center nuclear origin of replication recognition complex Biological process G1 phase DNA replication DNA replication initiation negative regulation of cell division G1/S transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 23594 56452 Ensembl ENSG00000091651 ENSMUSG00000031697 UniProt Q9Y5N6 Q9WUJ8 RefSeq (mRNA) NM_014321 NM_001163791 NM_019716 RefSeq (protein) NP_055136 n/a Location (UCSC) Chr 16: 46.69 – 46.7 Mb Chr 8: 86.03 – 86.03 Mb PubMed search [3] [4]
Wikidata
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Origin recognition complex subunit 6 (ORC6)
Identifiers
Symbol	ORC6
Pfam	PF05460
InterPro	IPR008721
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Origin recognition complex subunit 6 is a protein that in humans is encoded by the ORC6 (ORC6L) gene. ^{[5] [6]}

Background

The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins.

Function

The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and ORC1 are loosely associated with the core complex consisting of ORC2, -3, -4 and -5. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. ^[6]

Interactions

ORC6 has been shown to interact with MCM5, ^[7] ORC2, ^{[7] [8]} Replication protein A1, ^[7] ORC4, ^[7] DBF4, ^[7] ORC3, ^{[7] [8]} CDC45-related protein, ^[7] MCM4 ^[7] and Cell division cycle 7-related protein kinase. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000091651 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000031697 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Dhar SK, Dutta A (Nov 2000). "Identification and characterization of the human ORC6 homolog". J Biol Chem. 275 (45): 34983–8. doi: 10.1074/jbc.M006069200 . PMID   10945994.
6. "Entrez Gene: ORC6L origin recognition complex, subunit 6 like (yeast)".
7. Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (Mar 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1). England: 111–28. doi:10.1016/S0022-2836(03)00079-2. ISSN   0022-2836. PMID   12614612.
8. Vashee, S; Simancek P; Challberg M D; Kelly T J (Jul 2001). "Assembly of the human origin recognition complex". J. Biol. Chem. 276 (28). United States: 26666–73. doi: 10.1074/jbc.M102493200 . ISSN   0021-9258. PMID   11323433.

Further reading

• Li JJ, Herskowitz I (1994). "Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system". Science. 262 (5141): 1870–4. Bibcode:1993Sci...262.1870L. doi:10.1126/science.8266075. PMID   8266075.
• Jiang W, McDonald D, Hope TJ, Hunter T (1999). "Mammalian Cdc7-Dbf4 protein kinase complex is essential for initiation of DNA replication". EMBO J. 18 (20): 5703–13. doi:10.1093/emboj/18.20.5703. PMC   1171637 . PMID   10523313.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Vashee S, Simancek P, Challberg MD, Kelly TJ (2001). "Assembly of the human origin recognition complex". J. Biol. Chem. 276 (28): 26666–73. doi: 10.1074/jbc.M102493200 . PMID   11323433.
• Chesnokov I, Remus D, Botchan M (2001). "Functional analysis of mutant and wild-type Drosophila origin recognition complex". Proc. Natl. Acad. Sci. U.S.A. 98 (21): 11997–2002. Bibcode:2001PNAS...9811997C. doi: 10.1073/pnas.211342798 . PMC   59756 . PMID   11593009.
• Prasanth SG, Prasanth KV, Stillman B (2002). "Orc6 involved in DNA replication, chromosome segregation, and cytokinesis". Science. 297 (5583): 1026–31. Bibcode:2002Sci...297.1026P. doi:10.1126/science.1072802. PMID   12169736. S2CID   35758699.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Kneissl M, Pütter V, Szalay AA, Grummt F (2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. PMID   12614612.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Ramachandran N, Hainsworth E, Bhullar B, et al. (2004). "Self-assembling protein microarrays". Science. 305 (5680): 86–90. Bibcode:2004Sci...305...86R. doi:10.1126/science.1097639. PMID   15232106. S2CID   20936301.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to biology: a functional genomics pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC   528930 . PMID   15489336.
• Sibani S, Price GB, Zannis-Hadjopoulos M (2005). "Ku80 binds to human replication origins prior to the assembly of the ORC complex". Biochemistry. 44 (21): 7885–96. doi:10.1021/bi047327n. PMID   15910003.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC   1347501 . PMID   16381901.
• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983. S2CID   7827573.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human Origin recognition complex (ORC6)