Stephen Jackson | |
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Born | Stephen Philip Jackson 17 July 1962 Nottingham, England |
Alma mater | University of Leeds (BSc) Imperial College London (PhD) University of Edinburgh (PhD) |
Known for | Understanding cellular responses to DNA damage and their relevance to cancer |
Awards | EMBO member (1997) Fellow of the Royal Society (2008) FMedSci (2001) Buchanan Medal (2011) King Faisal International Prize (2016) Dr. A.H. Heineken Prize for Medicine (2016) Leopold Griffuel Prize (2019) |
Scientific career | |
Fields | Cancer DNA repair Cell biology Molecular biology |
Institutions | University of Cambridge University of California, Berkeley University of Edinburgh |
Thesis | Cloning and characterisation of the RNA8 gene of Saccharomyces cerevisiae (1987) |
Doctoral advisor | Jean Beggs |
Website | www www |
Sir Stephen Philip Jackson, FRS, FMedSci (born 17 July 1962) is the Frederick James Quick Professor of Biology. He is a senior group leader at the Cancer Research UK Cambridge Institute and associate group leader at the Gurdon Institute, University of Cambridge.
Jackson was educated at the University of Leeds, graduating with a Bachelor of Science degree in biochemistry in 1983. [1] He then carried out his PhD research working with Jean Beggs on yeast RNA splicing at Imperial College London and Edinburgh University, earning his PhD in 1987. [2]
Following his PhD, Jackson carried out postdoctoral research with Robert Tjian at the University of California, Berkeley, where he developed an interest in the regulation of transcription. He returned to the UK in 1991 as a Junior Group Leader at the then Wellcome-CRC Institute, now the Gurdon Institute.
Jackson's work has provided key insights into cellular processes that respond to DNA damage; processes fundamental to life and whose defects cause various diseases particularly cancer. [3] Through his discovery that the DNA-dependent protein kinase (DNA-PK) enzyme is activated by DNA double-strand breaks (DSBs), [4] Jackson's laboratory identified and characterised various components of the non-homologous end joining (NHEJ) system that repairs most DSBs in human cells. These studies also provided a paradigm for Jackson's later work on DNA-damage signalling by the ATM serine/threonine kinase and ATR (Ataxia telangiectasia and Rad3 related), [5] and his studies on how these and additional DNA repair factors interact with and influence one another, often in ways regulated by post-translational modifications. [6] Jackson's work has also helped establish how DSB repair is controlled during the cell cycle, at telomeres in response to cell aging/senescence, and within chromatin. [5] [6] [7] In 1997 Jackson founded KuDOS Pharmaceuticals with the aim of translating knowledge of DNA damage response pathways into new treatments for cancer. [8] KuDOS developed small-molecule inhibitors of several DNA damage response enzymes. The most advanced of these is the poly (ADP-ribose) polymerase 1 (PARP1) inhibitor Olaparib/Lynparza™, which is now a registered medicine worldwide. [9] [10] KuDOS developed into a fully integrated drug-discovery and drug-development company and was acquired by AstraZeneca in 2005. [11]
In 2011 Jackson founded MISSION Therapeutics [12] [13] a firm to develop drugs to improve the management of life-threatening diseases, particularly cancer. In 2017, Steve founded Adrestia Therapeutics Ltd [14] and currently serves as Chief Scientific Officer.
Jackson has received numerous awards, medals and honorary degrees: the inaugural Eppendorf-Nature European Young Investigator Award (1995); [15] the Tenovus Medal for Cancer Research (1997); [16] the Colworth Medal (1997); the Anthony Dipple Carcinogenesis Young Investigator award [17] (2002); the Biochemical Society GlaxoSmithKline Award (2008); [18] the BBSRC Innovator of the Year Award (2009); [19] the Royal Society Buchanan Medal (2011), [20] the latter in recognition of his "outstanding contributions to understanding DNA repair and DNA damage response signalling pathways", and the Gagna A. & Ch. Van Heck Prize [21] (2015) for "his cardinal contributions related to cellular events that detect, signal the presence of and repair DNA damages". Jackson is the co-winner of the King Faisal International Prize for Science 2016, in recognition of his "outstanding contribution to defining the link between the basic mechanism of genomic DNA instability and its relationship to cancer. Specifically, he unraveled the salient components of the pathway involved in DNA repair. He is also credited with an innovative approach to bring his findings into tangible therapeutic products to treat cancer". [22] He was elected a member of the European Molecular Biology Organization (EMBO) in 1997, a Fellow of the Academy of Medical Sciences in 2001 [23] and a Fellow of the Royal Society in 2008. [24] In 2016 Jackson was awarded the Dr A. H. Heineken Prize for Medicine [25] for his "fundamental research into DNA repair in human cells and for the successful application of knowledge of that process in the development of new cancer drugs". [26] In 2017 he was awarded the Genome Stability Network medal for his contributions to the field of genome stability and particularly for the realisation of the therapeutic potential of targeting the DDR. [27] The Fondation ARC's Leopold Griffuel Prize in Translational and Clinical Research was presented to Jackson in 2019 for his work on DNA damage repair and his role in the development of medicines such as PARP1 and 2 inhibitors, currently used for cancer treatment. [28] [29] In 2020, he was awarded the Royal Society's Mullard Award for his research on DNA repair mechanisms and synthetic lethality that led to the discovery of olaparib which has reached blockbuster status for the treatment of ovarian and breast cancers. [30] In 2022, Steve was awarded the Johann Anton Merck Award, which is given for outstanding scientific preclinical research accomplishments in the areas of Merck Healthcare‘s strategic focus. [31] Cancer Research UK honoured Steve with Cancer Research Horizon's Entrepreneurship Recognition Award in recognition of his longstanding academic entrepreneurship and his outstanding contributions that have enhanced the field of oncology. [32]
Jackson was knighted in the 2023 Birthday Honours for services to innovation and research. [33]
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as radiation can cause DNA damage, resulting in tens of thousands of individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages. This can eventually lead to malignant tumors, or cancer as per the two hit hypothesis.
In biochemistry, in the biological context of organisms' regulation of gene expression and production of gene products, downregulation is the process by which a cell decreases the production and quantities of its cellular components, such as RNA and proteins, in response to an external stimulus. The complementary process that involves increase in quantities of cellular components is called upregulation.
Non-homologous end joining (NHEJ) is a pathway that repairs double-strand breaks in DNA. NHEJ is referred to as "non-homologous" because the break ends are directly ligated without the need for a homologous template, in contrast to homology directed repair (HDR), which requires a homologous sequence to guide repair. NHEJ is active in both non-dividing and proliferating cells, while HDR is not readily accessible in non-dividing cells. The term "non-homologous end joining" was coined in 1996 by Moore and Haber.
Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death.
Homologous recombination is a type of genetic recombination in which genetic information is exchanged between two similar or identical molecules of double-stranded or single-stranded nucleic acids.
Chromatin remodeling is the dynamic modification of chromatin architecture to allow access of condensed genomic DNA to the regulatory transcription machinery proteins, and thereby control gene expression. Such remodeling is principally carried out by 1) covalent histone modifications by specific enzymes, e.g., histone acetyltransferases (HATs), deacetylases, methyltransferases, and kinases, and 2) ATP-dependent chromatin remodeling complexes which either move, eject or restructure nucleosomes. Besides actively regulating gene expression, dynamic remodeling of chromatin imparts an epigenetic regulatory role in several key biological processes, egg cells DNA replication and repair; apoptosis; chromosome segregation as well as development and pluripotency. Aberrations in chromatin remodeling proteins are found to be associated with human diseases, including cancer. Targeting chromatin remodeling pathways is currently evolving as a major therapeutic strategy in the treatment of several cancers.
ADP-ribosylation is the addition of one or more ADP-ribose moieties to a protein. It is a reversible post-translational modification that is involved in many cellular processes, including cell signaling, DNA repair, gene regulation and apoptosis. Improper ADP-ribosylation has been implicated in some forms of cancer. It is also the basis for the toxicity of bacterial compounds such as cholera toxin, diphtheria toxin, and others.
Ku70 is a protein that, in humans, is encoded by the XRCC6 gene.
Protein C-ets-1 is a protein that in humans is encoded by the ETS1 gene. The protein encoded by this gene belongs to the ETS family of transcription factors.
DNA-dependent protein kinase, catalytic subunit, also known as DNA-PKcs, is an enzyme that in humans is encoded by the gene designated as PRKDC or XRCC7. DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family. The DNA-Pkcs protein is a serine/threonine protein kinase consisting of a single polypeptide chain of 4,128 amino acids.
Poly [ADP-ribose] polymerase 1 (PARP-1) also known as NAD+ ADP-ribosyltransferase 1 or poly[ADP-ribose] synthase 1 is an enzyme that in humans is encoded by the PARP1 gene. It is the most abundant of the PARP family of enzymes, accounting for 90% of the NAD+ used by the family. PARP1 is mostly present in cell nucleus, but cytosolic fraction of this protein was also reported.
E3 ubiquitin-protein ligase RNF8 is an enzyme that in humans is encoded by the RNF8 gene. RNF8 has activity both in immune system functions and in DNA repair.
Olaparib, sold under the brand name Lynparza, is a medication for the maintenance treatment of BRCA-mutated advanced ovarian cancer in adults. It is a PARP inhibitor, inhibiting poly ADP ribose polymerase (PARP), an enzyme involved in DNA repair. It acts against cancers in people with hereditary BRCA1 or BRCA2 mutations, which include some ovarian, breast, and prostate cancers.
PARP inhibitors are a group of pharmacological inhibitors of the enzyme poly ADP ribose polymerase (PARP).
Simon Joseph Boulton is a British scientist who has made important contributions to the understanding of DNA repair and the treatment of cancer resulting from DNA damage. He currently occupies the position of Senior Scientist and group leader of the DSB Repair Metabolism Laboratory at the Francis Crick Institute, London. He is also an honorary Professor at University College London.
Dario Renato Alessi is a French-born British biochemist, Director of the Medical Research Council Protein Phosphorylation and Ubiquitylation Unit and Professor of Signal Transduction, at the School of Life Sciences, University of Dundee.
Tony Kouzarides, FMedSci, FRS is a senior group leader Gurdon Institute, a founding non-executive director of Abcam and a Professor of Cancer Biology at the University of Cambridge.
DNA damage is an alteration in the chemical structure of DNA, such as a break in a strand of DNA, a nucleobase missing from the backbone of DNA, or a chemically changed base such as 8-OHdG. DNA damage can occur naturally or via environmental factors, but is distinctly different from mutation, although both are types of error in DNA. DNA damage is an abnormal chemical structure in DNA, while a mutation is a change in the sequence of base pairs. DNA damages cause changes in the structure of the genetic material and prevents the replication mechanism from functioning and performing properly. The DNA damage response (DDR) is a complex signal transduction pathway which recognizes when DNA is damaged and initiates the cellular response to the damage.
PD-1 inhibitors and PD-L1 inhibitors are a group of checkpoint inhibitor anticancer drugs that block the activity of PD-1 and PDL1 immune checkpoint proteins present on the surface of cells. Immune checkpoint inhibitors are emerging as a front-line treatment for several types of cancer.
DNA end resection, also called 5′–3′ degradation, is a biochemical process where the blunt end of a section of double-stranded DNA (dsDNA) is modified by cutting away some nucleotides from the 5' end to produce a 3' single-stranded sequence. The presence of a section of single-stranded DNA (ssDNA) allows the broken end of the DNA to line up accurately with a matching sequence, so that it can be accurately repaired.