TRNAIle-lysidine synthase

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TRNAIle-lysidine synthase
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EC no. 6.3.4.19
CAS no. 635304-92-6
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TRNAIle-lysidine synthase (EC 6.3.4.19, TilS, mesJ (gene), yacA (gene), isoleucine-specific transfer ribonucleate lysidine synthetase, tRNAIle-lysidine synthetase) is an enzyme with systematic name L-lysine:(tRNAIle2)-cytidine34 ligase (AMP-forming). [1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction

[tRNAIle2]-cytidine34 + L-lysine + ATP [tRNAIle2]-lysidine34 + AMP + diphosphate + H2O

The bacterial enzyme modifies the wobble base of the CAU anticodon of tRNAIle at the oxo group in position 2 of cytidine34.

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References

  1. Ikeuchi Y, Soma A, Ote T, Kato J, Sekine Y, Suzuki T (July 2005). "molecular mechanism of lysidine synthesis that determines tRNA identity and codon recognition". Molecular Cell. 19 (2): 235–46. doi: 10.1016/j.molcel.2005.06.007 . PMID   16039592.
  2. Salowe SP, Wiltsie J, Hawkins JC, Sonatore LM (April 2009). "The catalytic flexibility of tRNAIle-lysidine synthetase can generate alternative tRNA substrates for isoleucyl-tRNA synthetase". The Journal of Biological Chemistry. 284 (15): 9656–62. doi: 10.1074/jbc.M809013200 . PMC   2665086 . PMID   19233850.
  3. Nakanishi K, Fukai S, Ikeuchi Y, Soma A, Sekine Y, Suzuki T, Nureki O (May 2005). "Structural basis for lysidine formation by ATP pyrophosphatase accompanied by a lysine-specific loop and a tRNA-recognition domain". Proceedings of the National Academy of Sciences of the United States of America. 102 (21): 7487–92. doi: 10.1073/pnas.0501003102 . PMC   1140429 . PMID   15894617.
  4. Soma A, Ikeuchi Y, Kanemasa S, Kobayashi K, Ogasawara N, Ote T, Kato J, Watanabe K, Sekine Y, Suzuki T (September 2003). "An RNA-modifying enzyme that governs both the codon and amino acid specificities of isoleucine tRNA". Molecular Cell. 12 (3): 689–98. doi: 10.1016/s1097-2765(03)00346-0 . PMID   14527414.
  5. Nakanishi K, Bonnefond L, Kimura S, Suzuki T, Ishitani R, Nureki O (October 2009). "Structural basis for translational fidelity ensured by transfer RNA lysidine synthetase". Nature. 461 (7267): 1144–8. doi:10.1038/nature08474. PMID   19847269. S2CID   4426738.