Coprinopsis cinerea

Last updated

Coprinopsis cinerea
Coprinopsis spec. - Lindsey 2a.jpg
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Fungi
Division: Basidiomycota
Class: Agaricomycetes
Order: Agaricales
Family: Psathyrellaceae
Genus: Coprinopsis
Species:
C. cinerea
Binomial name
Coprinopsis cinerea
(Schaeff.) Redhead, Vilgalys & Moncalvo (2001)
Synonyms [1]

Agaricus cinereusSchaeff. (1774)
Agaricus radians Batsch (1783)
Agaricus macrorhizus Pers. (1796)
Coprinus cinereus(Schaeff.) Gray (1821)
Coprinus fimetarius var. cinereus(Schaeff.) Fr. (1838)
Coprinus fimetarius var. macrorhizus(Pers.) Sacc. (1887)
Coprinus macrorhizus(Pers.) Rea (1922)
Coprinus delicatulus Apinis (1965)
Hormographiella aspergillataGuarro, Gené & De Vroey (1992)

Contents

Coprinopsis cinerea
Information icon.svg
Gills icon.png Gills on hymenium
Conical cap icon.svg Cap is conical
Free gills icon2.svg Hymenium is free
Bare stipe icon.svg Stipe is bare
Transparent spore print icon.svg
Spore print is black
Saprotrophic fungus.svgEcology is saprotrophic
Mycomorphbox Edible.pngEdibility is edible

Coprinopsis cinerea is a species of mushroom in the family Psathyrellaceae. Commonly known as the gray shag, it is edible, but must be used promptly after collecting. [2]

Coprinopsis cinerea is an important model organism for studying fungal sex and mating types, mushroom development, [3] and the evolution of multicellularity of fungi. The genome sequence was published in 2010. [4] It is considered to be particularly suited organism to study meiosis, due to its synchronous meiotic development and prolonged prophase. [5]

Research

Antibiotics

Researchers in 2014 discovered a protein produced by Coprinopsis cinerea with antibiotic properties. [6] The protein, known as copsin, has similar effects to other non-protein organically derived antibiotics. To date, it has not been determined whether antibiotic medicine for humans and other animals can be developed from this protein.

Culturing

Coprinopsis cinerea can be grown on complex (e.g. YMG, YMG/T) or minimal media (e.g. mKjalke medium), solid or liquid, with or without agitation, at 25 °C or optimally at 37 °C. It can be grown in dark or with 12-h light/12-h dark cycle. [7] [8]

Strains

Genome

Coprinopsis cinerea strain Okayama 7 (#130) was sequenced with 10x coverage in 2003. A third and most recent revision of the sequence of strain Okayama 7 (#130) was released in 2010. Its haploid genome is ca. 37.5 Mb. [10]

Molecular cloning

Coprinopsis cinerea can be transformed with exogenous DNA by transformation when the fungus is a protoplast. It was found that disrupting (knockout or RNAi silencing) ku70 homologue can increase gene targeting via increased homologous recombination. Either protoplasts derived from oidia or vegetative mycelium can be used, however, gene targeting was found to be higher by 2% (based on phenotyping) when using vegetative mycelium. [7] [8] Otherwise, insertion of integrative vectors ectopically and with small homologous regions can be used, likely with low transformation efficiency. Earlier, REMI (restriction enzyme-mediated integration) could be used to insert exogenous DNA into the chromosome to produce mutant strains. This relies on inserting exogenous DNA and restriction enzymes into the protoplast cell, allowing for the enzymes to cut the chromosome at specific sites which match those sites used to produce linearized plasmid DNA with the gene of interest; subsequently, host enzymes ligate the cut sites and thus produce integrated heterologous, exogenous DNA. Although successful, undesirable mutations are likely. [9] Chemical mutagenesis (also random) can also be done. Phenotype selection of the inability to fruit can indicate that insertion led to disruption of vital genes. All in all, homologous recombination provides more specificity when creating a mutant strain. Depending on the mutant, auxotrophy markers (requires lost gene to be inserted) or prototrophy (when causing essential gene deletion) be used for selection.

Enzymes

Coprinopsis cinerea is known to produce laccase, a type of phenoloxidase. C. cinerea produces a variety of the same laccase, known as isoenzymes. Laccase activity can be measured by zymograms (in which a substrate for the enzyme is present in a separating gel). Under stressed conditions, temperature and medium, laccase secretion was increased. Although copper is required co-factor for laccase, merely adding copper did not induce laccase secretion. [11] It was recently found that a TET (Ten-Eleven translocation dioxygenases) homologue, CcTET, was identified in C. cinerea, which may have important human (or mammalian) implications like cancer. DNA methylation is vital in humans and dysfunction is associated with cancer, thus, studying methylation reactions in non-mammalians may provide better insight into mammalian methylation reactions. [12]

Reproduction

Coprinopsis cinerea can sense blue light. It was identified that gene Cc.wc-2 is involved in blue light photoreception. [7] Etiolated stipes (elongation without cap maturation) is caused when grown without light. [13]

Meiosis

Coprinopsis cinerea is an ideal model for studying meiosis because meiosis progresses synchronously in about 10 million cells within each mushroom cap. [14] Meiosis is a specialized cell division process, occurring in diploid cells, in which a single round of DNA replication occurs, and is followed by two divisions to produce four haploid daughter nuclei. During meiosis homologous chromosomes pair with each other and undergo a DNA repair process in which DNA damage is removed and genetic information is recombined.[ citation needed ] Burns et al. [14] studied the expression of genes involved in the 15-hour meiotic process encompassing time points prior to the haploid nuclear fusion that forms the diploid zygote to the final formation of the four haploid products. They compared expression of particular genes in C. cinerea to the expression of the comparable genes (orthologs) in two other species ( Saccharomyces cerevisiae and Schizosaccharomyces pombe ) from which C. cinerea had diverged in evolution 500 to 900 million years ago. They found that the expression of individual genes turned on or off at the same stage in C. cinerea as in the other two species. They also found that genes considered to be specifically involved in the meiotic process were more conserved in their expression pattern than non-meiotic genes. These findings indicate ancient conservation of the meiotic process.

Human disease

Coprinopsis cinerea is harmless to human and animal health under normal conditions. However, the organism can cause opportunistic infections (mycoses) in immunocompromised patients, such as those who have undergone haematopoietic stem cell transplantation or are otherwise undergoing immunosuppression treatment. [15] Most reported cases have been respiratory infections, but cases involving the heart, skin, brain or gut have been reported, and the infections may rapidly become systemic. [16] Whilst exceptionally rare, Coprinopsis cinerea infection is difficult to treat and often fatal in this vulnerable patient group. The infection is caused by the mould-like asexual (non mushroom-forming) anamorph of Coprinopsis cinerea which used to be known as Hormographiella aspergillata, and may be described under this name in the clinical literature. [17] [18]

See also

Related Research Articles

<span class="mw-page-title-main">Meiosis</span> Cell division producing haploid gametes

Meiosis is a special type of cell division of germ cells in sexually-reproducing organisms that produces the gametes, such as sperm or egg cells. It involves two rounds of division that ultimately result in four cells with only one copy of each chromosome (haploid). Additionally, prior to the division, genetic material from the paternal and maternal copies of each chromosome is crossed over, creating new combinations of code on each chromosome. Later on, during fertilisation, the haploid cells produced by meiosis from a male and a female will fuse to create a cell with two copies of each chromosome again, the zygote.

<span class="mw-page-title-main">Basidiomycota</span> Division of fungi

Basidiomycota is one of two large divisions that, together with the Ascomycota, constitute the subkingdom Dikarya within the kingdom Fungi. Members are known as basidiomycetes. More specifically, Basidiomycota includes these groups: agarics, puffballs, stinkhorns, bracket fungi, other polypores, jelly fungi, boletes, chanterelles, earth stars, smuts, bunts, rusts, mirror yeasts, and Cryptococcus, the human pathogenic yeast. Basidiomycota are filamentous fungi composed of hyphae and reproduce sexually via the formation of specialized club-shaped end cells called basidia that normally bear external meiospores. These specialized spores are called basidiospores. However, some Basidiomycota are obligate asexual reproducers. Basidiomycota that reproduce asexually can typically be recognized as members of this division by gross similarity to others, by the formation of a distinctive anatomical feature, cell wall components, and definitively by phylogenetic molecular analysis of DNA sequence data.

<span class="mw-page-title-main">Genetic recombination</span> Production of offspring with combinations of traits that differ from those found in either parent

Genetic recombination is the exchange of genetic material between different organisms which leads to production of offspring with combinations of traits that differ from those found in either parent. In eukaryotes, genetic recombination during meiosis can lead to a novel set of genetic information that can be further passed on from parents to offspring. Most recombination occurs naturally and can be classified into two types: (1) interchromosomal recombination, occurring through independent assortment of alleles whose loci are on different but homologous chromosomes ; & (2) intrachromosomal recombination, occurring through crossing over.

<i>Saccharomyces cerevisiae</i> Species of yeast

Saccharomyces cerevisiae is a species of yeast. The species has been instrumental in winemaking, baking, and brewing since ancient times. It is believed to have been originally isolated from the skin of grapes. It is one of the most intensively studied eukaryotic model organisms in molecular and cell biology, much like Escherichia coli as the model bacterium. It is the microorganism behind the most common type of fermentation. S. cerevisiae cells are round to ovoid, 5–10 μm in diameter. It reproduces by budding.

<span class="mw-page-title-main">Homologous chromosome</span> Chromosomes that pair in fertilization

A couple of homologous chromosomes, or homologs, are a set of one maternal and one paternal chromosome that pair up with each other inside a cell during fertilization. Homologs have the same genes in the same loci where they provide points along each chromosome which enable a pair of chromosomes to align correctly with each other before separating during meiosis. This is the basis for Mendelian inheritance which characterizes inheritance patterns of genetic material from an organism to its offspring parent developmental cell at the given time and area.

<i>Entamoeba histolytica</i> Anaerobic parasitic protist

Entamoeba histolytica is an anaerobic parasitic amoebozoan, part of the genus Entamoeba. Predominantly infecting humans and other primates causing amoebiasis, E. histolytica is estimated to infect about 35-50 million people worldwide. E. histolytica infection is estimated to kill more than 55,000 people each year. Previously, it was thought that 10% of the world population was infected, but these figures predate the recognition that at least 90% of these infections were due to a second species, E. dispar. Mammals such as dogs and cats can become infected transiently, but are not thought to contribute significantly to transmission.

<span class="mw-page-title-main">Transformation (genetics)</span> Genetic alteration of a cell by uptake of genetic material from the environment

In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). For transformation to take place, the recipient bacterium must be in a state of competence, which might occur in nature as a time-limited response to environmental conditions such as starvation and cell density, and may also be induced in a laboratory.

<i>Neurospora crassa</i> Species of ascomycete fungus in the family Sordariaceae

Neurospora crassa is a type of red bread mold of the phylum Ascomycota. The genus name, meaning 'nerve spore' in Greek, refers to the characteristic striations on the spores. The first published account of this fungus was from an infestation of French bakeries in 1843.

<span class="mw-page-title-main">Mating of yeast</span> Biological process

The yeast Saccharomyces cerevisiae is a simple single-celled eukaryote with both a diploid and haploid mode of existence. The mating of yeast only occurs between haploids, which can be either the a or α (alpha) mating type and thus display simple sexual differentiation. Mating type is determined by a single locus, MAT, which in turn governs the sexual behaviour of both haploid and diploid cells. Through a form of genetic recombination, haploid yeast can switch mating type as often as every cell cycle.

A meiocyte is a type of cell that differentiates into a gamete through the process of meiosis. Through meiosis, the diploid meiocyte divides into four genetically different haploid gametes. The control of the meiocyte through the meiotic cell cycle varies between different groups of organisms.

<span class="mw-page-title-main">Mating in fungi</span> Combination of genetic material between compatible mating types

Fungi are a diverse group of organisms that employ a huge variety of reproductive strategies, ranging from fully asexual to almost exclusively sexual species. Most species can reproduce both sexually and asexually, alternating between haploid and diploid forms. This contrasts with many eukaryotes such as mammals, where the adults are always diploid and produce haploid gametes which combine to form the next generation. In fungi, both haploid and diploid forms can reproduce – haploid individuals can undergo asexual reproduction while diploid forms can produce gametes that combine to give rise to the next generation.

Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics studies microorganisms for different purposes. The microorganisms that are observed are bacteria, and archaea. Some fungi and protozoa are also subjects used to study in this field. The studies of microorganisms involve studies of genotype and expression system. Genotypes are the inherited compositions of an organism. Genetic Engineering is a field of work and study within microbial genetics. The usage of recombinant DNA technology is a process of this work. The process involves creating recombinant DNA molecules through manipulating a DNA sequence. That DNA created is then in contact with a host organism. Cloning is also an example of genetic engineering.

<span class="mw-page-title-main">Lorna Casselton</span> British geneticist, academic and educator

Lorna Ann Casselton, was a British academic and biologist. She was Professor Emeritus of Fungal Genetics in the Department of Plant Science at the University of Oxford, and was known for her genetic and molecular analysis of the mushroom Coprinus cinereus and Coprinus lagopus.

<span class="mw-page-title-main">Tetrad (meiosis)</span>

The tetrad is the four spores produced after meiosis of a yeast or other Ascomycota, Chlamydomonas or other alga, or a plant. After parent haploids mate, they produce diploids. Under appropriate environmental conditions, diploids sporulate and undergo meiosis. The meiotic products, spores, remain packaged in the parental cell body to produce the tetrad.

Chromosome segregation is the process in eukaryotes by which two sister chromatids formed as a consequence of DNA replication, or paired homologous chromosomes, separate from each other and migrate to opposite poles of the nucleus. This segregation process occurs during both mitosis and meiosis. Chromosome segregation also occurs in prokaryotes. However, in contrast to eukaryotic chromosome segregation, replication and segregation are not temporally separated. Instead segregation occurs progressively following replication.

<span class="mw-page-title-main">DMC1 (gene)</span> Protein-coding gene in the species Homo sapiens

Meiotic recombination protein DMC1/LIM15 homolog is a protein that in humans is encoded by the DMC1 gene.

<i>Coprinopsis lagopus</i> Species of fungus

Coprinopsis lagopus is a species of fungus in the family Psathyrellaceae. Until 2001, the species was known as Coprinus lagopus; advances in the understanding of phylogenetic relationships between the various coprinoid species led to a major reorganization of that genus. It is a delicate and short-lived fungus, the fruit bodies lasting only a few hours before dissolving into a black ink – a process called deliquescence. The vague resemblance of the young fruit body to the paw of a white rabbit has earned this species the common name harefoot mushroom.

The origin and function of meiosis are currently not well understood scientifically, and would provide fundamental insight into the evolution of sexual reproduction in eukaryotes. There is no current consensus among biologists on the questions of how sex in eukaryotes arose in evolution, what basic function sexual reproduction serves, and why it is maintained, given the basic two-fold cost of sex. It is clear that it evolved over 1.2 billion years ago, and that almost all species which are descendants of the original sexually reproducing species are still sexual reproducers, including plants, fungi, and animals.

<i>Podospora anserina</i> Species of fungus

Podospora anserina is a filamentous ascomycete fungus from the order Sordariales. It is considered a model organism for the study of molecular biology of senescence (aging), prions, sexual reproduction, and meiotic drive. It has an obligate sexual and pseudohomothallic life cycle. It is a non-pathogenic coprophilous fungus that colonizes the dung of herbivorous animals such as horses, rabbits, cows and sheep.

Cospin is a serine protease inhibitor from the mushroom species Coprinopsis cinerea in the phylum Basidiomycota.

References

  1. "Coprinopsis cinerea (Schaeff.) Redhead, Vilgalys & Moncalvo 2001". MycoBank. International Mycological Association. Retrieved 2011-01-16.
  2. McKnight VB, McKnight KH (1987). A Field Guide to Mushrooms: North America. Peterson Field Guides. Boston, Massachusetts: Houghton Mifflin. p. 277. ISBN   978-0-395-91090-0.
  3. Kües, U (2000). "Life history and developmental processes in the basidiomycete Coprinus cinereus". Microbiology and Molecular Biology Reviews. 64 (2): 316–53. doi:10.1128/MMBR.64.2.316-353.2000. PMC   98996 . PMID   10839819.
  4. Stajich JE, Wilke SK, Ahren D, et al. (June 29, 2010). "Insights into evolution of multicellular fungi from the assembled chromosomes of the mushroom Coprinopsis cinerea (Coprinus cinereus)". PNAS. 107 (26): 11889–11894. doi: 10.1073/pnas.1003391107 . PMC   2900686 . PMID   20547848.
  5. Burns C, Stajich JE, Rechtsteiner A, et al. (2010). "Analysis of the basidiomycete Coprinopsis cinerea reveals conservation of the core meiotic expression program over half a billion years of evolution". PLOS Genetics. 6 (9): e1001135. doi: 10.1371/journal.pgen.1001135 . PMC   2944786 . PMID   20885784.
  6. "New antibiotic in mushroom that grows on horse dung". 7 November 2014.
  7. 1 2 3 Efficient gene targeting in ΔCc.ku70 or ΔCc.lig4 mutants of the agaricomycete Coprinopsis cinerea.
  8. 1 2 Lcc1 and Lcc5 are the main laccases secreted in liquid cultures of Coprinopsis cinerea strains
  9. 1 2 Restriction enzyme-mediated DNA integration in Coprinus cinereus
  10. "Coprinopsis cinerea Genome Project". 15 May 2015.
  11. Lcc1 and Lcc5 are the main laccases secreted in liquid cultures of Coprinopsis cinerea strains
  12. A TET Homologue Protein from Coprinopsis cinerea (CcTET) That Biochemically Converts 5‑Methylcytosine to 5‑Hydroxymethylcytosine, 5‑Formylcytosine, and 5‑Carboxylcytosine
  13. Life History and Developmental Processes in the Basidiomycete Coprinus cinereus
  14. 1 2 Burns C, Stajich JE, Rechtsteiner A, Casselton L, Hanlon SE, Wilke SK, Savytskyy OP, Gathman AC, Lilly WW, Lieb JD, Zolan ME, Pukkila PJ (September 2010). "Analysis of the Basidiomycete Coprinopsis cinerea reveals conservation of the core meiotic expression program over half a billion years of evolution". PLOS Genet. 6 (9): e1001135. doi: 10.1371/journal.pgen.1001135 . PMC   2944786 . PMID   20885784.
  15. Bojic M, Willinger B, Rath T, Tobudic S, Thalhammer F, Böhm A, Mitterbauer M, Schulenburg A, Wöhrer S, Kalhs P, Rabitsch W. Fatal skin and pulmonary infection caused by H ormographiella aspergillata in a leukaemic patient: case report and literature overview. Mycoses. 2013 Nov;56(6):687-9.
  16. Correa-Martinez C, Brentrup A, Hess K, Becker K, Groll AH, Schaumburg F. First description of a local Coprinopsis cinerea skin and soft tissue infection. New microbes and new infections. 2018 Jan 1;21:102-4.
  17. Bojic M, Willinger B, Rath T, Tobudic S, Thalhammer F, Böhm A, Mitterbauer M, Schulenburg A, Wöhrer S, Kalhs P, Rabitsch W. Fatal skin and pulmonary infection caused by H ormographiella aspergillata in a leukaemic patient: case report and literature overview. Mycoses. 2013 Nov;56(6):687-9.
  18. Correa-Martinez C, Brentrup A, Hess K, Becker K, Groll AH, Schaumburg F. First description of a local Coprinopsis cinerea skin and soft tissue infection. New microbes and new infections. 2018 Jan 1;21:102-4.