Lactococcus garvieae | |
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Scientific classification | |
Domain: | Bacteria |
Phylum: | Bacillota |
Class: | Bacilli |
Order: | Lactobacillales |
Family: | Streptococcaceae |
Genus: | Lactococcus |
Species: | L. garvieae |
Binomial name | |
Lactococcus garvieae (Collins et al., 1984) Schleifer et al., 1986 | |
Lactococcus garvieae is a known fish pathogen affecting saltwater fish in the Far East, specifically in rainbow trout, Japanese yellowtail, Cobia (Rachycentron canadum) [1] and grey mullet (Mugil cephalus). This bacteria causes lesions in the vascular endothelium, leading to hemorrhages and petechias at the surface of internal organs. [2] As few as 10 bacterial cells per fish can cause an infection. L. garvieae is isolated in saltwater fish in the Far East and specifically in European rainbow trout. [3]
Lactococcus garvieae is usually identified within aquatic species. However, it has also been found in subclinical intramammary infections in cows, subclinical mastitis in water buffalos, poultry meat, raw cow's milk, meat products, porcine blood from industrial abattoirs and from cat and dog tonsils. [2] Rare cases have occurred involving infection in humans with a 25% mortality rate. [4]
Lactococcus garvieae was first discovered in rainbow trout raised on a Japanese fish farm in the 1950s. In 1988, L. garvieae was identified in the rainbow trout from Spanish fish farms as well. In later years, L. garvieae was isolated from several septicemic processes in fish and phenotypical and molecular taxonomic studies confirmed the same agent as E. seriolicida. This species was reclassified as a junior synonym of L. garvieae. [5]
Lactococcus garvieae is a facultatively anaerobic, non-motile, non-spore forming, Gram-positive ovoid coccus, occurring in pairs and short chains. It can produce α-hemolysis on blood agar (BA). It has the ability to grow at 4-45 °C in media containing 6.5% sodium chloride (NaCl) at pH 9.6. Its optimal growth temperature is 37 °C for a 24‑hour period, while at 4 °C it needs between 12 and 15 days for premium growth. It also grows rapidly in rich media, such as brain heart infusion agar (BHIA), trypticase soy agar (TSA), BA, trypticase soy broth (TSB), and bile esculin agar (BEA). Conversely, L. garvieae does not grow on McConkey agar or Enterococcus agar. Optimum conditions for colony growth exists within brain heart infusion broth (BHIB) where conditions include a pH range of 7-8 and a temperature range of 25-30 °C. [5]
In fish models, all clinical forms of lactococcosis show an absence of clinical symptoms and mortalities in fish weighing under 80 grams. Smaller fish can be infected experimentally. In a follow-up study of the pathogenicity of L. garvieae, younger fish at 50 grams underwent a higher mortality than older fish at about 100 grams and the acute period of the disease was reported to be longer in young fish. [5]
Numerous investigations of L. garvieae pathogenicity have confirmed that capsulated strains, commonly classified as serotype KG−, are more virulent than non-capsulated strains, commonly classified as serotype KG+. The appearance of the disease is affected specifically by factors of the aquatic environment such as temperature and water quality. [5]
Water temperature affects the disease seasonally as the climate changes, specifically when the water temperature is over 18 °C (64 °F). Infection is linked to water temperatures over 18 °C, although acute outbreaks have been discovered in water temperatures of 14–15 °C. [5]
Low water quality caused by poor sanitary conditions has been shown to influence evolution of infection. The disease becomes more distinct when the immediate aquatic environment is poor, and oxygen deficiency increases virulence of the agent. Likewise, excessive ammonium concentration causes an increase in mortality of fish. [5]
Several virulence experiments have been performed in order to determine the possible correlation between pathogenicity of L. garvieae in rainbow trout and the two antigenic profiles (KG- and KG+). The results revealed that capsulated strains (KG-) were more virulent than non-capsulated (KG+), showing LD50 values as low as 102 bacteria per fish. The KG− type strain was more virulent than the KG+ because when the surface morphologies of the KG− and KG+ phenotypes were differentiated by scanning electron microscopy, KG− cells were found to be more hydrophilic than KG+ cells. The immune response of Japanese yellowtail following injection of the two phenotypes varied with higher adhering titers in the KG+ phenotype compared to the KG-. [5]
Successively, 24 isolates of L. garvieae from different fish species and geographic origin were studied by slide cohesion tests. These tests were conducted using rabbit antisera against representative strains with diverse origins and by Dot blot assays. These results endorsed the establishment of two different groups of isolates, but a correlation between serological group and geographic origin or host source could not be determined. [5]
Barnes and Ellis serologically compared 17 geographically distinct strains of L. garvieae isolated from diseased rainbow trout, finding that sera raised against capsule deficient isolates did not agglutinate capsulated isolates, whereas all antisera against capsulated strains cross reacted with non-capsulated isolates. The results determine that L. garvieae can be differentiated serologically into three different serotypes: a European capsulated serotype, a Japanese capsulated serotype and a non-capsulated serotype from both the European and Japanese regions. [5]
The comparative analysis of genomes of a virulent strain Lg2 (KG-) and a non-virulent strain ATCC 49156 (KG+) of L. garvieae revealed that the two strains shared a high degree of sequence identity, but Lg2 had a 16.5-kb capsule gene cluster that is absent in ATCC 49156. [6] The capsule gene cluster of Lg2 may be a genomic island from several features such as the presence of insertion sequences flanked on both ends, different GC content from the chromosomal average, integration into the locus syntenic to other lactococcal genome sequences, and distribution in human gut microbiomes. [6]
In fish, the incubation period of L. garvieae is very brief and the microorganism performs with high virulence. In an experimental infection by intraperitoneal route in Japanese yellowtail, it caused symptoms 2–3 days post-inoculation, while intramuscular infection in grey mullet ( Mugil cephalus ) produced its first symptoms and fatalities two days post-inoculation. Correspondingly, intraperitoneal experimental infection in rainbow trout caused the first symptoms and deaths three days post-inoculation. [5] Warm water lactococcosis has been detected in a variety of host organisms, including cage cultured cobia, grey mullet, freshwater prawn and Tilapia.
The gross pathology of lactococcosis arises with the presence of a rapid and general anorexia, melanosis, lethargy, loss of orientation, and irregular swimming. Typical external symptoms include exophthalmia and the presence of hemorrhages in the periorbital and intraocular area, the base of fins, the perianal region, the opercula and the buccal region. In further studies, swollen abdomens and anal prolapsus have been observed. Due to infection, fish have produced lesions in the vascular endothelium that cause blood extravasation, leading to hemorrhages and petechiae at the surface of internal organs. The main organs affected are the spleen, liver, brain, stomach, kidney and heart. [5]
Macroscopic lesions in affected fish are typical of acute systemic disease with strong congestion in the internal organs and different levels of hemorrhages in the swim bladder, intestine, liver, peritoneum, spleen and kidney. Also, enlargement of the spleen, focal areas of necrosis in the liver and spleen, pericarditis, hemorrhagic fluid in the intestine, and yellowish exudate covering the brain surface are typically observed. [5]
Histopathology is found mainly in the eyes and internal organs’ capsules. Lesions on the ocular area consist of extensive fibroplasias with inflammatory cells penetration. In the brain, lesions exist in the cerebrum and cerebellum. Diseased fish typically show signs of acute meningitis, consisting of an exudate covering the brain surface. In the heart, lesions are usually signified by fibroplasias, macrophage, and lymphocyte. In kidneys, the renal tubules have hyaline droplet deposition in the epithelia and hyaline casts in the lumen. [5]
According to a series of studies in 2006, L. garvieae is an emerging pathogen that is causing significant economic losses both in marine and freshwater aquaculture when water temperature increases over 16 °C in summer months. This pathogen causes serious economic losses due to three main factors: elevated rates of mortality have been investigated at 50% of fish, a decrease in the growth rates of fish due to infection, and unpleasant appearance of the infected fish which makes them unmarketable to consumers. [5]
Several vaccines have been developed to tackle L. garvieae infection in the face of rising antibiotic resistance, with whole-cell killed cells being the most common. [7]
Lactococcus garvieae in humans is a rare pathogen and of low virulence. More than 31 cases of infection in humans have been reported. These include 25 cases of endocarditis and other infections like those related to peritoneal dialysis catheters, discitis, catheter associated UTI, post TURP infection, liver abscess in a patient with cholangiocarcinoma, AICD/Pacemaker related infections to name a few. [3]
The signs and symptoms of United States cases ranges from urinary tract, blood, skin and pneumonic processes. In Canada, patients have been found with bacterial endocarditis. It is speculated that the infection followed the consumption of fresh seafood and is believed to be facilitated by immunosuppression or liver cirrhosis. [2] A patient with L. garvieae septicaemia in absence of infective endocarditis was successfully treated with a combination of ampicillin and gentamicin and showed a favourable clinical course. Antibiotic therapy adapted to the antibiogram (levofloxacin, amoxicillin, and Clavulanic acid) for eight weeks and an oral anticoagulative therapy for three months. [3]
Bacterial endocarditis from L. garvieae is extremely rare and may actually be underreported due to its morphologic and biochemical similarities with enterococci. [8] The source of infection in many L. garvieae infected patients is unclear, where similar cases were identified and patients denied having contact with domestic animals or fish, or eating raw fish, milk or meat and dairy products. However, a few cases had known diverticulitis of the colon, which could provide a point of entry for L. garvieae infection. [9]
Streptococcus is a genus of gram-positive or spherical bacteria that belongs to the family Streptococcaceae, within the order Lactobacillales, in the phylum Bacillota. Cell division in streptococci occurs along a single axis, thus when growing they tend to form pairs or chains, which may appear bent or twisted. This differs from staphylococci, which divide along multiple axes, thereby generating irregular, grape-like clusters of cells. Most streptococci are oxidase-negative and catalase-negative, and many are facultative anaerobes.
Salmonella is a genus of rod-shaped, (bacillus) gram-negative bacteria of the family Enterobacteriaceae. The two known species of Salmonella are Salmonella enterica and Salmonella bongori. S. enterica is the type species and is further divided into six subspecies that include over 2,650 serotypes. Salmonella was named after Daniel Elmer Salmon (1850–1914), an American veterinary surgeon.
Escherichia coli O157:H7 is a serotype of the bacterial species Escherichia coli and is one of the Shiga-like toxin–producing types of E. coli. It is a cause of disease, typically foodborne illness, through consumption of contaminated and raw food, including raw milk and undercooked ground beef. Infection with this type of pathogenic bacteria may lead to hemorrhagic diarrhea, and to kidney failure; these have been reported to cause the deaths of children younger than five years of age, of elderly patients, and of patients whose immune systems are otherwise compromised.
Shigella is a genus of bacteria that is Gram negative, facultatively anaerobic, non–spore-forming, nonmotile, rod shaped, and is genetically nested within Escherichia. The genus is named after Kiyoshi Shiga, who discovered it in 1897.
Virulence is a pathogen's or microorganism's ability to cause damage to a host.
Listeria monocytogenes is the species of pathogenic bacteria that causes the infection listeriosis. It is a facultative anaerobic bacterium, capable of surviving in the presence or absence of oxygen. It can grow and reproduce inside the host's cells and is one of the most virulent foodborne pathogens. Twenty to thirty percent of foodborne listeriosis infections in high-risk individuals may be fatal. In the European Union, listeriosis continues an upward trend that began in 2008, causing 2,161 confirmed cases and 210 reported deaths in 2014, 16% more than in 2013. In the EU, listeriosis mortality rates also are higher than those of other foodborne pathogens. Responsible for an estimated 1,600 illnesses and 260 deaths in the United States annually, listeriosis ranks third in total number of deaths among foodborne bacterial pathogens, with fatality rates exceeding even Salmonella spp. and Clostridium botulinum.
Lactococcus is a genus of lactic acid bacteria that were formerly included in the genus Streptococcus Group N1. They are known as homofermenters meaning that they produce a single product, lactic acid in this case, as the major or only product of glucose fermentation. Their homofermentative character can be altered by adjusting environmental conditions such as pH, glucose concentration, and nutrient limitation. They are gram-positive, catalase-negative, non-motile cocci that are found singly, in pairs, or in chains. The genus contains strains known to grow at or below 7˚C.
Yersinia enterocolitica is a Gram-negative, rod-shaped bacterium, belonging to the family Yersiniaceae. It is motile at temperatures of 22–29°C (72–84°F), but becomes nonmotile at normal human body temperature. Y. enterocolitica infection causes the disease yersiniosis, which is an animal-borne disease occurring in humans, as well as in a wide array of animals such as cattle, deer, pigs, and birds. Many of these animals recover from the disease and become carriers; these are potential sources of contagion despite showing no signs of disease. The bacterium infects the host by sticking to its cells using trimeric autotransporter adhesins.
Infectious bursal disease (IBD), also known as Gumboro disease, infectious bursitis, and infectious avian nephrosis, is a highly contagious disease of young chickens and turkeys caused by infectious bursal disease virus (IBDV), characterized by immunosuppression and mortality generally at 3 to 6 weeks of age. The disease was first discovered in Gumboro, Delaware in 1962. It is economically important to the poultry industry worldwide due to increased susceptibility to other diseases and negative interference with effective vaccination. In recent years, very virulent strains of IBDV (vvIBDV), causing severe mortality in chicken, have emerged in Europe, Latin America, South-East Asia, Africa, and the Middle East. Infection is via the oro-fecal route, with affected birds excreting high levels of the virus for approximately 2 weeks after infection. The disease is easily spread from infected chickens to healthy chickens through food, water, and physical contact.
Aeromonas hydrophila is a heterotrophic, Gram-negative, rod-shaped bacterium mainly found in areas with a warm climate. This bacterium can be found in fresh or brackish water. It can survive in aerobic and anaerobic environments, and can digest materials such as gelatin and hemoglobin. A. hydrophila was isolated from humans and animals in the 1950s. It is the best known of the species of Aeromonas. It is resistant to most common antibiotics and cold temperatures and is oxidase- and indole-positive. Aeromonas hydrophila also has a symbiotic relationship as gut flora inside of certain leeches, such as Hirudo medicinalis.
Pasteurella multocida is a Gram-negative, nonmotile, penicillin-sensitive coccobacillus of the family Pasteurellaceae. Strains of the species are currently classified into five serogroups based on capsular composition and 16 somatic serovars (1–16). P. multocida is the cause of a range of diseases in mammals and birds, including fowl cholera in poultry, atrophic rhinitis in pigs, and bovine hemorrhagic septicemia in cattle and buffalo. It can also cause a zoonotic infection in humans, which typically is a result of bites or scratches from domestic pets. Many mammals and birds harbor it as part of their normal respiratory microbiota.
Dichelobacter nodosus, formerly Bacteroides nodosus, is a Gram-negative, obligate anaerobe of the family Cardiobacteriaceae. It has polar fimbriae and is the causative agent of ovine foot rot as well as interdigital dermatitis. It is the lone species in the genus Dichelobacter.
Infectious hematopoietic necrosis virus (IHNV), is a negative-sense single-stranded, bullet-shaped RNA virus that is a member of the Rhabdoviridae family, and from the genus Novirhabdovirus. It causes the disease known as infectious hematopoietic necrosis in salmonid fish such as trout and salmon. The disease may be referred to by a number of other names such as Chinook salmon disease, Coleman disease, Columbia River sockeye disease, Cultus Lake virus disease, Oregon sockeye disease, Sacramento River Chinook disease and sockeye salmon viral disease. IHNV is commonly found in the Pacific Coast of Canada and the United States, and has also been found in Europe and Japan. The first reported epidemics of IHNV occurred in the United States at the Washington and the Oregon fish hatcheries during the 1950s. IHNV is transmitted following shedding of the virus in the feces, urine, sexual fluids, and external mucus and by direct contact or close contact with the surrounding water. The virus gains entry into fish at the base of the fins.
Aeromonas salmonicida is a pathogenic bacterium that severely impacts salmonid populations and other species. It was first discovered in a Bavarian brown trout hatchery by Emmerich and Weibel in 1894. Aeromonas salmonicida's ability to infect a variety of hosts, multiply, and adapt, make it a prime virulent bacterium. A. salmonicida is an etiological agent for furunculosis, a disease that causes sepsis, haemorrhages, muscle lesions, inflammation of the lower intestine, spleen enlargement, and death in freshwater fish populations. It is found worldwide with the exception of South America. The major route of contamination is poor water quality; however, it can also be associated stress factors such as overcrowding, high temperatures, and trauma. Spawning and smolting fish are prime victims of furunculosis due to their immunocompromised state of being.
Streptococcus iniae is a species of Gram-positive, sphere-shaped bacterium belonging to the genus Streptococcus. Since its isolation from an Amazon freshwater dolphin in the 1970s, S. iniae has emerged as a leading fish pathogen in aquaculture operations worldwide, resulting in over US$100M in annual losses. Since its discovery, S. iniae infections have been reported in at least 27 species of cultured or wild fish from around the world. Freshwater and saltwater fish including tilapia, red drum, hybrid striped bass, and rainbow trout are among those susceptible to infection by S. iniae. Infections in fish manifest as meningoencephalitis, skin lesions, and septicemia.
Vibrio anguillarum is a species of prokaryote that belongs to the family Vibrionaceae, genus Vibrio. V. anguillarum is typically 0.5 - 1 μm in diameter and 1 - 3 μm in length. It is a gram-negative, comma-shaped rod bacterium that is commonly found in seawater and brackish waters. It is polarly flagellated, non-spore-forming, halophilic, and facultatively anaerobic. V. anguillarum has the ability to form biofilms. V. anguillarum is pathogenic to various fish species, crustaceans, and mollusks.
Actinobacillus pleuropneumoniae, is a Gram-negative, facultative anaerobic, respiratory pathogen found in pigs. It was first reported in 1957, and was formally declared to be the causative agent of porcine pleuropneumonia in 1964. It was reclassified in 1983 after DNA studies showed it was more closely related to A. lignieresii.
Infectious pancreatic necrosis virus (IPNV) is a double-stranded RNA virus from the family Birnaviridae, in the genus Aquabirnavirus. Causing the highly infectious disease Infectious pancreatic necrosis, the virus primarily affects young salmonids resulting in high mortality, occasionally surpassing 90 percent in the early stages. IPNV or IPNV-like viruses have been isolated worldwide from at least 32 families of saltwater and freshwater salmonids and non-salmonids fish including salmon, flatfish, pike, eels and others. Other aquatic organisms infected include 11 molluscs and 4 species of crustaceans. Due to its wide host range and high mortality, the virus is of great concern to global aquaculture. In addition to persistence in hosts, IPNV is also perpetual in the environment, surviving across a range of conditions and capable of infecting fish with as little as 101TCID50/ml of the virus. Found in Europe, North America, South America, Africa, Asia, and Australia, the virus has led to significant losses in the mariculture of Atlantic salmon, brook trout, and rainbow trout.
Piscirickettsia salmonis is the bacterial causative agent of piscirickettsiosis, an epizootic disease in salmonid fishes. It has a major impact on salmon populations, with a mortality rate of up to 90% in some species. The type strain, LF-89, is from Chile, but multiple strains exist, and some are more virulent than others. P. salmonis and piscrickettsiosis are present in various geographic regions from Europe to Oceania to South America, but the Chilean salmon farming industry has been particularly hard-hit. Different strategies of controlling the disease and farm-to-farm spread have been the subject of much research, but a significant amount is still unknown.
LG2 or variation, may refer to: