Route Road Name(s) From To Notes CR 53 US 27 (SR 20 ) / CR 534 in Buckville CR 53 at the Madison County line north-northeast of Day Former SR 53 [1] CR 138A CR 490 north-northeast of Hatchbend Riverside Drive north-northeast of Hatchbend Unsigned CR 250 CR 53 north-northeast of Day CR 250 at the Suwannee County line north-northeast of Day Former SR 250 [1] CR 251 US 27 (SR 20 ) / CR 350 southeast of Buckville CR 250 north-northeast of Day Former SR 251 [1] CR 260 CR 53 north-northeast of Day CR 251 northeast of Day Former SR 348C [1] CR 270 CR 53 / NW Maine Avenue in Day CR 251 east of Day Former SR 348B [1] CR 280 CR 53 / NW Gulfwind Avenue south of Day CR 251 southeast of Day CR 290 CR 53 south of Day CR 292 / Ezell Landing Road at Allen Mill Pond Conservation Area southeast of Day Former SR 348A [1] CR 292 US 27 (SR 20 ) southeast of Buckville CR 290 / Ezell Landing Road at Allen Mill Pond Conservation Area southeast of Day Former SR 251B [1] CR 300 CR 534 northwest of Mayo US 27 (SR 20 ) / NW Bloxham Street in Mayo Unsigned except on street signs; former SR 251 (north of CR 320 ) and SR 251A (south of CR 320) [1] CR 320 CR 300 west-northwest of Mayo US 27 (SR 20 ) northwest of Mayo Former SR 251 [1] CR 340 CR 355 south-southeast of Mayo Shaw Road / Parker Avenue east-southeast of Mayo former SR 251 [1] CR 348 US 27 (SR 20 ) west of Buckville CR 53 north-northeast of Buckville Former SR 348 [1] CR 350 CR 534 / SW Arlington Road south of Buckville US 27 (SR 20 ) / CR 251 southeast of Buckville Former SR 350 [1] CR 351 CR 534 south-southeast of Buckville CR 350 / SW Dickens Road southeast of Buckville Former SR 350 [1] CR 353 US 27 (SR 20 ) / W.R. Williams Road east of Alton CR 354 north-northeast of Alton CR 354 SR 51 / Savannah Road north of Mayo US 27 (SR 20 ) / CR 405 east of Alton Former SR 354 [1] CR 355 Pine Street CR 360 south-southeast of Mayo SR 51 / Pine Street in Mayo Former SR 355 [1] CR 357 CR 357 at the Dixie County line south-southeast of Cooks Hammock SR 51 in Cooks Hammock former SR 357 [1] CR 360 SR 51 south-southwest of Mayo CR 405 south of Midway Former SR 355 (east of CR 355 ) [1] and SR 355A (west of CR 355)[ citation needed ] CR 361 US 27 (SR 20 ) / CR 371 east of Alton CR 354 / NE Pecan Avenue northeast of Alton CR 371 SE Knox Lake Road southeast of Alton US 27 (SR 20 ) / CR 361 east of Alton CR 400 SR 51 north of Mayo US 27 (SR 20 ) east of Mayo CR 405 US 27 (SR 20 ) west-northwest of Grady US 27 (SR 20 ) / CR 354 east of Alton Former SR 354 [1] CR 410 US 27 (SR 20 ) / Walter Henderson Road east-southeast of Alton Owen Springs Boat Ramp on the Suwannee River east-southeast of Alton Former SR 251 (east of CR 411 ) [1] CR 411 US 27 (SR 20 ) southeast of Alton CR 410 east-southeast of Alton Former SR 251 [1] CR 412 US 27 (SR 20 southeast of Alton US 27 (SR 20 east-southeast of Alton Former SR 251 [1] CR 416 CR 405 southeast of Alton US 27 (SR 20 ) east-southeast of Alton Former SR 354A [1] CR 420 CR 405 west-northwest of Grady US 27 (SR 20 ) / Mandarin Road west-northwest of Grady CR 425 US 27 (SR 20 ) northwest of Grady Orchid Road / Jeff Walker Road west-northwest of Grady CR 450 US 27 (SR 20 ) Mandarin Road / Lavender Road / C-420 CR 475 US 27 (SR 20 ) west-northwest of Grady SR 349 west-southwest of Grady CR 480 SR 349 Boat Ramp along the Suwannee River Former SR 138 (east of CR 500 ) and SR 342 (west of CR 500) [1] CR 500 SR 349 west of Hatchbend CR 480 north-northwest of Hatchbend Former SR 342 [1] CR 534 US 27 (SR 20 ) / CR 53 SR 51 / CR 536 / NE Suwannee Acres Lane north of Mayo Former SR 53 [1] CR 536 US 27 (SR 20 ) northwest of Mayo SR 51 / CR 534 / NE Suwannee Acres Lane north of Mayo Former SR 53 [1]