Metarhizium

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Metarhizium
Metarhizium anisopliae infected cockroach (PLoS).jpg
Cockroach killed by Metarhizium anisopliae
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Sordariomycetes
Subclass: Hypocreomycetidae
Order: Hypocreales
Family: Clavicipitaceae
Genus: Metarhizium
Sorokīn, 1879
Type species
Metarhizium anisopliae
(Metchnikoff) Sorokīn

Metarhizium [1] is a genus of entomopathogenic fungi in the Clavicipitaceae family. With the advent of genetic profiling, placing these fungi in proper taxa has now become possible. Most turn out to be the asexual forms (anamorphs) of fungi in the phylum Ascomycota, including Metacordyceps spp.

Species

Before molecular techniques were introduced at the end of the 20th century, Metarhizium species were identified on morphological (notably conidial) characteristics. The 'original' species included: M. anisopliae (with M.a. var. major ), M. brunneum , M. cicadinum , M. cylindrosporum , M. flavoviride , M. taii , M. truncatum , and M. viridicolumnare . In 2009, nine former varieties of the type species M. anisopliae were assigned species status. [2] New species have continued to be identified, with original names sometimes re-instated (notably M. brunneum ). The first complete chromosome length genome sequence for any Metarhizium was carried-out for this species at Swansea University in 2021. [3]

The index fungorum currently (April 2024) lists: [4]

  1. Metarhizium acridum (Driver & Milner) J.F. Bisch., S.A. Rehner & Humber (2009)
  2. Metarhizium alvesii R.B. Lopes, M. Faria, C. Montalva & R.A. Humber (2018)
  3. Metarhizium anisopliae (Metschn.) Sorokīn (1883)
  4. Metarhizium argentinense A.C. Gutierrez, A. Leclerque & C.C. Lopez Lastra (2019)
  5. Metarhizium atrovirens (Kobayasi & Shimizu) Kepler, S.A. Rehner & Humber (2014)
  6. Metarhizium baoshanense Z.H. Chen, Ling Xu, X.N. Yang, Y.G. Zhang & Y.M. Yang (2018)
  7. Metarhizium bibionidarum Nishi & Hiroki Sato (2017)
  8. Metarhizium biotecense Luangsa-ard, Khons., Thanakitp. & Samson (2020)
  9. Metarhizium blattodeae C. Montalva, Humber, K. Collier & C. Luz (2016)
  10. Metarhizium brachyspermum Koh. Yamam., Ohmae & Orihara (2019)
  11. Metarhizium brasiliense Kepler, S.A. Rehner & Humber (2014)
  12. Metarhizium brittlebankisoides (Zuo Y. Liu, Z.Q. Liang, Whalley, Y.J. Yao & A.Y. Liu) Kepler, S.A. Rehner & Humber (2014)
  13. Metarhizium brunneum Petch (1935)
  14. Metarhizium campsosterni (W.M. Zhang & T.H. Li) Kepler, S.A. Rehner & Humber (2014)
  15. Metarhizium candelabrum Luangsa-ard, Mongkols., Thanakitp. & Samson (2020)
  16. Metarhizium cercopidarum Luangsa-ard, Mongkols., Thanakitp. & Samson (2020)
  17. Metarhizium chaiyaphumense Tasan., Khons., Thanak., Mongkols. & Luangsa-ard (2017)
  18. Metarhizium cicadae Luangsa-ard, Tasan., Thanakitp. & Samson (2020)
  19. Metarhizium cicadinum (Höhn.) Petch (1931)
  20. Metarhizium clavatum Luangsa-ard, Mongkols., Lamlertthon, Thanakitp. & Samson (2020)
  21. Metarhizium culicidarum Luangsa-ard, Khons., Thanakitp. & Samson (2020)
  22. Metarhizium cylindrosporum Q.T. Chen & H.L. Guo (1986)
  23. Metarhizium dendrolimi Z.Q. Liang, Wan H. Chen, Y.F. Han & X. Zou (2017)
  24. Metarhizium eburneum Luangsa-ard, Noisrip., Thanakitp. & Samson (2020)
  25. Metarhizium ellipsoideum Luangsa-ard, Khons., Thanakitp. & Samson (2020)
  26. Metarhizium flavoviride W. Gams & Rozsypal (1973)
  27. Metarhizium flavum Luangsa-ard, Mongkols., Thanakitp. & Samson (2020)
  28. Metarhizium frigidum J.F. Bisch. & S.A. Rehner (2007)
  29. Metarhizium fusoideum Luangsa-ard, Mongkols., Thanakitp. & Samson (2020)
  30. Metarhizium gaoligongense Z.H. Chen & L. Xu (2018)
  31. Metarhizium globosum J.F. Bisch., S.A. Rehner & Humber (2009)
  32. Metarhizium granulomatis (Sigler) Kepler, S.A. Rehner & Humber (2014)
  33. Metarhizium gryllidicola Khons., Thanakitp. & Luangsa-ard (2020)
  34. Metarhizium guniujiangense (C.R. Li, B. Huang, M.Z. Fan & Z.Z. Li) Kepler, S.A. Rehner & Humber (2014)
  35. Metarhizium huainamdangense Luangsa-ard, Mongkols., Thanakitp. & Samson (2020)
  36. Metarhizium humberi C. Luz, L. Rocha & I. Delalibera, (2019)
  37. Metarhizium indigoticum (Kobayasi & Shimizu) Kepler, S.A. Rehner & Humber (2014)
  38. Metarhizium kalasinense Tasan., Khons., Thanak., Mongkols. & Luangsa-ard (2017)
  39. Metarhizium koreanum Kepler, S.A. Rehner & Humber (2014)
  40. Metarhizium lepidiotae (Driver & Milner) J.F. Bisch., S.A. Rehner & Humber (2009)
  41. Metarhizium lepidopterorum W.H. Chen, Y.F. Han, J.D. Liang & Z.Q. Liang (2019)
  42. Metarhizium macrosemiae M.J. Chen & B. Huang (2022)
  43. Metarhizium majus (J.R. Johnst.) J.F. Bisch., S.A. Rehner & Humber (2009)
  44. Metarhizium megapomponiae Luangsa-ard, Tasan., Thanakitp. & Samson (2020)
  45. Metarhizium minus (Rombach, Humber & D.W. Roberts) Kepler, S.A. Rehner & Humber (2014)
  46. Metarhizium niveum Luangsa-ard, Tasan., Thanakitp. & Samson (2020)
  47. Metarhizium nornnoi Luangsa-ard, Khons., Thanakitp. & Samson (2020)
  48. Metarhizium novozealandicum (Driver & Milner) Kepler, S.A. Rehner & Humber (2014)
  49. Metarhizium ovoidosporum Luangsa-ard, Khons., Thanakitp. & Samson (2020)
  50. Metarhizium owariense (Kobayasi) Kepler, S.A. Rehner & Humber (2014)
  51. Metarhizium pemphigi (Driver & Milner) Kepler, Humber & S.A. Rehner (2014)
  52. Metarhizium phasmatodeae Khons., Thanakitp. & Luangsa-ard (2020)
  53. Metarhizium phuwiangense Luangsa-ard, Mongkols., Himaman, Thanakitp. & Samson (2020)
  54. Metarhizium prachinense Tasan., Khons., Thanak., Mongkols. & Luangsa-ard (2017)
  55. Metarhizium pseudoatrovirens (Kobayasi & Shimizu) Kepler, S.A. Rehner & Humber (2014)
  56. Metarhizium purpureogenum Nishi, S. Shimizu & Hiroki Sato (2017)
  57. Metarhizium purpureonigrum Luangsa-ard, Tasan., Thanakitp. & Samson (2020)
  58. Metarhizium purpureum Luangsa-ard, Mongkols., Lamlertthon, Thanakitp. & Samson (2020)
  59. Metarhizium putuoense Yi Li, X.C. Zhao, Yu Wang & W.F. Lin (2023)
  60. Metarhizium reniforme (Samson & H.C. Evans) Luangsa-ard, Boucias & Hywel-Jones (2017)
  61. Metarhizium rileyi (Farl.) Kepler, S.A. Rehner & Humber (2014)
  62. Metarhizium robertsii J.F. Bisch., S.A. Rehner & Humber (2009)
  63. Metarhizium rongjiangense W.H. Chen, Y.F. Han, J.D. Liang & Z.Q. Liang (2019)
  64. Metarhizium samlanense Luangsa-ard, Thanak., Tasan., Mongkols. & Hywel-Jones (2017)
  65. Metarhizium sulphureum Luangsa-ard, Khons., Thanakitp. & Samson (2020)
  66. Metarhizium synnematis Z.Q. Liang, H.L. Chu & T.C. Wen (2016)
  67. Metarhizium taii Z.Q. Liang & A.Y. Liu (1991)
  68. Metarhizium takense Tasan., Thanak., Mongkols. & Luangsa-ard (2017)
  69. Metarhizium truncatum Petch (1931)
  70. Metarhizium viride (Segretain, Fromentin, Destombes, Brygoo & Dodin ex Samson) Kepler, S.A. Rehner & Humber (2014)
  71. Metarhizium viridicolumnare (Matsush.) Matsush. (1993)
  72. Metarhizium viridulum (Tzean, L.S. Hsieh, J.L. Chen & W.J. Wu) B. Huang & Z.Z. Li (2004)

Other Reclassified Species Names

Teleomorphs

The teleomorphs of Metarhizium species appear to be members of the genus Metacordyceps . [7] Metacordyceps taii (as Cordyceps taii) has been described as the teleomorph of Metarhizium taii: [8] [9] a name that has now been restored.

Whether the other varieties of M. anisopliae have their own teleomorphs is not yet clear. Some, if not most, strains of M. anisopliae possibly have lost the capability of reproducing sexually.

Natural pesticide

The artificially grown fungi's spores are also used as a natural pesticide. Certain strains are advised against use in food-growing fields and in close proximity to water sources due to risk of their contamination. [10] [note 1]

Locust control

In the 1990s, the LUBILOSA research programme proved that M. acridum in its spore form was effective in killing locusts and other members of the Acrididea families with no deleterious effects found in field trials on any nontarget species except for the domesticated silk worm Bombyx mori . [11] It is currently produced as a biopesticide under the name Novacrid by the company Eléphant Vert in their factory in Meknès, Morocco. [12] The same company recently (2019) obtained the licence to produce and market the original product developed by LUBILOSA, which is called Green Muscle. A third product, Green Guard, is produced by BASF of Australia for the control of Australian plague locusts and wingless grasshoppers. [13]

Notes

  1. In this case only Metarhizium anisopliae F52 strain is concerned.

Related Research Articles

<i>Ophiocordyceps sinensis</i> Species of fungus

Ophiocordyceps sinensis, known colloquially as caterpillar fungus, is an entomopathogenic fungus in the family Ophiocordycipitaceae. It is mainly found in the meadows above 3,500 metres (11,500 ft) on the Tibetan Plateau in Tibet and the Himalayan regions of Bhutan, India, and Nepal. It parasitizes larvae of ghost moths and produces a fruiting body which is valued in traditional Chinese medicine as an aphrodisiac. Caterpillar fungus contains the compound cordycepin, an adenosine derivative. However, the fruiting bodies harvested in nature usually contain high amounts of arsenic and other heavy metals, so they are potentially toxic and sales have been strictly regulated by China's State Administration for Market Regulation since 2016.

<i>Cordyceps</i> Genus of fungi

Cordyceps is a genus of ascomycete fungi that includes about 600 worldwide species. Diverse variants of cordyceps have had more than 1,500 years of use in Chinese medicine. Most Cordyceps species are endoparasitoids, parasitic mainly on insects and other arthropods ; a few are parasitic on other fungi.

<i>Beauveria bassiana</i> Species of fungus

Beauveria bassiana is a fungus that grows naturally in soils throughout the world and acts as a parasite on various arthropod species, causing white muscardine disease; it thus belongs to the group of entomopathogenic fungi. It is used as a biological insecticide to control a number of pests, including termites, thrips, whiteflies, aphids and various beetles. Its use in the control of bed bugs and malaria-transmitting mosquitos is under investigation.

<span class="mw-page-title-main">Hypocreales</span> Order of fungi

The Hypocreales are an order of fungi within the class Sordariomycetes. In 2008, it was estimated that it contained some 237 genera, and 2647 species in seven families. Since then, a considerable number of further taxa have been identified, including an additional family, the Stachybotryaceae. Wijayawardene et al. in 2020 added more families and genera to the order. According to the Catalog of Life, As of April 2021 the Hypocreales contains 6 families, 137 genera, and 1411 species. Hyde et al. (2020a) listed 14 families under Hypocreales, while, Wijayawardene et al. (2022) accepted 15 families in the order, where Cylindriaceae was additionally added. Earlier, Hyde et al. (2020a) had placed Cylindriaceae in class Xylariomycetidae. Samarakoon et al. (2022) agreed. Hence, Cylindriaceae should have been excluded from Hypocreales and placed in Xylariomycetidae. Xiao et al. (2022) recently introduced a new family Polycephalomycetaceae to Hypocreales.

Metarhizium robertsii is a fungus that grows naturally in soils throughout the world and causes disease in various insects by acting as a parasitoid. It is a mitosporic fungus with asexual reproduction, which was formerly classified in the form class Hyphomycetes of the phylum Deuteromycota.

<i>Paecilomyces</i> Genus of fungi

Paecilomyces is a genus of fungi. A number of species in this genus are plant pathogens.

<span class="mw-page-title-main">Clavicipitaceae</span> Family of fungi

The Clavicipitaceae are a family of fungi within the order Hypocreales. A 2008 estimate placed 43 genera in the family, but a study in 2020 has increased this number to 50.

<i>Beauveria</i> Genus of fungi

Beauveria is a genus of asexually-reproducing fungi allied with the ascomycete family Cordycipitaceae. Its several species are typically insect pathogens. The sexual states (teleomorphs) of Beauveria species, where known, are species of Cordyceps.

<span class="mw-page-title-main">LUBILOSA</span>

LUBILOSA was the name of a research programme that aimed at developing a biological alternative to the chemical control of locusts. This name is an acronym of the French title of the programme: Lutte Biologique contre les Locustes et les Sauteriaux. During its 13-year life, the programme identified an isolate of an entomopathogenic fungus belonging to the genus Metarhizium and virulent to locusts, and went through all the necessary steps to develop the commercial biopesticide product Green Muscle based on its spores.

<i>Metarhizium acridum</i> Grasshopper- and locust-killing fungus

Metarhizium acridum is the new name given to a group of fungal isolates that are known to be virulent and specific to the Acrididea (grasshoppers). Previously, this species has had variety status in Metarhizium anisopliae ; before that, reference had been made to M. flavoviride or Metarhizium sp. describing an "apparently homologous and distinctive group" of isolates that were most virulent against Schistocerca gregaria in early screening bioassays.

<i>Metarhizium majus</i> Species of fungus

Metarhizium majus is the name given to a group of fungal isolates that are known to be virulent against Scarabaeidae, a family of beetles. Previously, this species has had variety status in Metarhizium anisopliae and its name is derived from characteristically very large spores for the genus Metarhizium. There has been considerable interest in developing isolates of this species into mycoinsecticides: especially against the coconut and oil palm beetle pest Oryctes in SE Asia, the Pacific region and Africa.

<i>Metacordyceps</i> Genus of fungi

Metacordyceps is a genus of fungi in the family Clavicipitaceae. The anamorphs of Metacordyceps appear to include Metarhizium species.

<i>Tolypocladium ophioglossoides</i> Species of fungus

Tolypocladium ophioglossoides, also known by two of its better known synonyms Elaphocordyceps ophioglossoides and Cordyceps ophioglossoides and commonly known as the goldenthread cordyceps, is a species of fungus in the family Ophiocordycipitaceae. It is parasitic on fruit bodies of the truffle-like Elaphomyces. The species is considered inedible, but is valued in traditional Chinese medicine.

Metarhizium brunneum is the re-instated name of a group of reassigned Metarhizium isolates, previously grouped in the species "Metarhizium anisopliae var. anisopliae": based on a multigene phylogenetic approach using near-complete sequences from nuclear DNA. It is a mitosporic fungus with asexual reproduction, which was formerly classified in the form class Hyphomycetes of the form phylum Deuteromycota. M. brunneum has been isolated from Coleoptera, Lepidoptera, Diptera and soil samples, but a commercially developed isolate (below) has proved virulent against Hemiptera and Thysanoptera.

<span class="mw-page-title-main">Cordycipitaceae</span> Family of fungi

The Cordycipitaceae are a family of parasitic fungi in the Ascomycota, class Sordariomycetes and order Hypocreales. The family was first published in 1969 by mycologist Hanns Kreisel, but the naming was invalid according to the code of International Code of Nomenclature for algae, fungi, and plants. It was validly published in 2007.

<i>Isaria</i> Genus of fungi

Isaria is a genus of fungi mostly in the order Hypocreales and family Clavicipitaceae, or by some authorities the Cordycipitaceae. It includes a large number of entomopathogenic species, some of them exploited as biopesticides : often previously assigned to the genus Paecilomyces.

Metarhizium rileyi is a species of entomopathogenic fungus in the family Clavicipitaceae. This species is known to infect Lepidoptera, including economically important insects in the Noctuoidea and Bombycoidea; there is an extensive literature on this fungus under its synonym Nomuraea rileyi.

<i>Metarhizium anisopliae</i> Species of fungus

Metarhizium anisopliae is the type species in its genus of fungi, that grows naturally in soils throughout the world and causes disease in various insects by acting as a parasitoid. Ilya I. Mechnikov named it Entomophthora anisopliae (basionym) after the insect species from which it was originally isolated – the beetle Anisoplia austriaca and from these early days, fungi such as this have been seen as potentially important tools for pest management. It is a mitosporic fungus with asexual reproduction, which was formerly classified in the form class Hyphomycetes of the phylum Deuteromycota.

<i>Cordyceps locustiphila</i> Species of fungus

Cordyceps locustiphila is the basionym and teleomorph of the fungi Beauveria locustiphila, a species of fungus in the family Cordycipitaceae. and is a species within the genus Cordyceps. It was originally described in by Henn in 1904. C. locustiphila is an entomopathogen and obligate parasite of the grasshopper species within the genus Colpolopha or Tropidacris, and as such is endemic to South America. The scientific name is derived from its close relationship with its host, being named after locusts. The fungi was renamed to Beauveria locustiphila in 2017 following research into the family Cordycipitaceae. Following the loss of the species type specimen, new studies were conducted that now recommend that the fungi be divided into 3 species. C. locustiphila, C. diapheromeriphila, and C. acridophila.

<i>Cordyceps gunnii</i> Species of fungus

Cordyceps gunni is a species of fungus in the family Cordycipitaceae, and is of the genus Cordyceps. It was originally found and recorded by Gunn in Tasmania and named as Sphaeria gunnii and later moved into the Cordyceps genus and renamed Cordyceps gunnii. This fungus and its sisters in the genus Cordyceps are known for growing out of insect bodies. C. gunnii can be found at ground level poking out of caterpillar burrows, attached to a caterpillar's head.

References

  1. Sorokīn (1879) Veg. Parasitenk. Mensch Tieren2: 268.
  2. Bischoff J.F.; Rehner S.A. Humber R.A. (2009). "A multilocus phylogeny of the Metarhizium anisopliae lineage". Mycologia. 101 (4): 512–530. doi:10.3852/07-202. PMID   19623931.
  3. Saud, Z.; Kortsinoglou, A.M.; Kouvelis, V.N.; Butt, T.M. (2021). "Telomere length de novo assembly of all 7 chromosomes and mitogenome sequencing of the model entomopathogenic fungus, Metarhizium brunneum, by means of a novel assembly pipeline". BMC Genomics. 22 (1): 87. doi: 10.1186/s12864-021-07390-y . PMC   7842015 . PMID   33509090.
  4. 1 2 Species fungorum search Metarhizium (retrieved 27 April 2024)
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  7. Sung, G.-H.; Hywel-Jones, N.L.; Sung, J.-M.; Luangsa-ard, J.J.; Shrestha, B. & Spatafora, J.W. (2007). "Phylogenetic classification of Cordyceps and the clavicipitaceous fungi". Studies in Mycology. 57: 5–59. doi:10.3114/sim.2007.57.01. PMC   2104736 . PMID   18490993.
  8. Liang, Z.-Q.; Liu, A.-Y.; Liu, J.-L. (1991). "A new species of the genus Cordyceps and its Metarhizium anamorph". Acta Mycologica Sinica. 10: 257–262.
  9. Huang B.; Li C.; Humber R.A.; Hodge K.T.; Fan M.; Li Z. (2005). "Molecular evidence for the taxonomic status of Metarhizium taii and its teleomorph, Cordyceps taii (Hypocreales, Clavicipitaceae)" (PDF). Mycotaxon. 94: 137–147.
  10. "Metarhizium anisopliae strain F52 (029056) Biopesticide Fact Sheet" (PDF). Pennsylvania: Environmental Protection Agency. June 1, 2003.
  11. Lomer, C.J.; Bateman, R.P.; Johnson, D.L.; Langewald, J.; Thomas, M. (2001). "Biological Control of Locusts and Grasshoppers". Annual Review of Entomology. 46: 667–702. doi:10.1146/annurev.ento.46.1.667. PMID   11112183.
  12. Eléphant Vert company website
  13. BASF website