Methyl violet is a family of organic compounds that are mainly used as dyes. Depending on the number of attached methyl groups, the color of the dye can be altered. Its main use is as a purple dye for textiles and to give deep violet colors in paint and ink. It is also used as a hydration indicator for silica gel. Methyl violet 10B is also known as crystal violet (and many other names) and has medical uses. [1]
The term methyl violet encompasses three compounds that differ in the number of methyl groups attached to the amine functional group. Methyl violets are mixtures of tetramethyl (2B), pentamethyl (6B) and hexamethyl (10B) pararosanilins. [2]
They are all soluble in water, ethanol, diethylene glycol and dipropylene glycol.
Name | Methyl violet 2B | Methyl violet 6B | Methyl violet 10B (Crystal violet) |
---|---|---|---|
Structure | |||
Formula (salt) | C23H26ClN3 | C24H28ClN3 | C25H30ClN3 |
CAS no | 84215-49-6 | 8004-87-3 | 548-62-9 |
C.I. | 42536 | 42535 | 42555 |
ChemSpider ID | 21164086 | 170606 | 10588 |
PubChem ID | 91997555 | 164877 | 11057 |
Formula (cation) | C23H26N3+ | C24H28N3+ | C25H30N3+ |
ChemSpider ID | 2006225 | 3349, 9080056, 10354393 | |
PubChem ID | 2724053 | 3468 |
Methyl violet 2B (IUPAC name: '4,4'-((4-Iminocyclohexa-2,5-dien-1-ylidene)methylene)bis(N,N-dimethylaniline) monohydrochloride) is a green powder which is soluble in water and ethanol but not in xylene. It appears yellow in solution of low pH (~0.15) and changes to violet with pH increasing toward 3.2. [3]
Methyl violet 10B has six methyl groups. It is known in medicine as Gentian violet (or crystal violet or pyoctanin(e) [1] ) and is the active ingredient in a Gram stain, used to classify bacteria. It is used as a pH indicator, with a range between 0 and 1.6. The protonated form (found in acidic conditions) is yellow, turning blue-violet above pH levels of 1.6. [4]
Methyl violet 10B inhibits the growth of many Gram positive bacteria, except streptococci.[ citation needed ] When used in conjunction with nalidixic acid (which destroys gram-negative bacteria), it can be used to isolate the streptococci bacteria for the diagnosis of an infection.[ citation needed ]
Methyl violet is a mutagen and mitotic poison, therefore concerns exist regarding the ecological impact of the release of methyl violet into the environment. Methyl violet has been used in vast quantities for textile and paper dyeing, and 15% of such dyes produced worldwide are released to environment in wastewater. Numerous methods have been developed to treat methyl violet pollution. The three most prominent are chemical bleaching, biodegradation, and photodegradation.
Chemical bleaching is achieved by oxidation or reduction. Oxidation can destroy the dye completely, e.g. through the use of sodium hypochlorite (NaClO, common bleach) or hydrogen peroxide. [5] [6] Reduction of methyl violet occurs in microorganisms but can be attained chemically using sodium dithionite.
Biodegradation has been well investigated because of its relevance to sewage plants with specialized microorganisms. Two microorganisms that have been studied in depth are the white rot fungus and the bacterium Nocardia Corallina . [7] [8]
Light alone does not rapidly degrade methyl violet, [9] but the process is accelerated upon the addition of large band-gap semiconductors, TiO2 or ZnO. [10] [11]
Many other methods have been developed to treat the contamination of dyes in a solution, including electrochemical degradation, [12] ion exchange, [13] laser degradation, and absorption onto various solids such as activated charcoal.
Biodegradation is the breakdown of organic matter by microorganisms, such as bacteria and fungi. It is generally assumed to be a natural process, which differentiates it from composting. Composting is a human-driven process in which biodegradation occurs under a specific set of circumstances.
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Crystal violet or gentian violet, also known as methyl violet 10B or hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic (vermicide) properties and was formerly important as a topical antiseptic. The medical use of the dye has been largely superseded by more modern drugs, although it is still listed by the World Health Organization.
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Methyl violet 6B is a violet triarylmethane dye from the group of cationic dyes and an essential component of C.I. Basic Violet 1. The compound is sometimes equated with methyl violet in the literature.
Methyl violet 2B is a violet triarylmethane dye from the group of cationic dyes and an essential component of C.I. Basic Violet 1. Methyl violets are mixtures of tetramethyl (2B), pentamethyl (6B) and hexamethyl (10B) pararosanilins.
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