Phosphate solubilizing bacteria

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Phosphate solubilizing bacteria cultured in Petri dish. The zone of n clearance can be clearly seen. Phosphate solubilizing bacteria cultured in petri dish.jpg
Phosphate solubilizing bacteria cultured in Petri dish. The zone of n clearance can be clearly seen.

Phosphate solubilizing bacteria (PSB) are beneficial bacteria capable of solubilizing inorganic phosphorus from insoluble compounds. [1] P-solubilization ability of rhizosphere microorganisms is considered to be one of the most important traits associated with plant phosphate nutrition. It is generally accepted that the mechanism of mineral phosphate solubilization by PSB strains is associated with the release of low molecular weight organic acids, through which their hydroxyl and carboxyl groups chelate the cations [an ion that have positive charge on it.] bound to phosphate, thereby converting it into soluble forms. PSB have been introduced to the Agricultural community as phosphate Biofertilizer. Phosphorus (P) is one of the major essential macronutrients for plants and is applied to soil in the form of phosphate fertilizers. However, a large portion of soluble inorganic phosphate which is applied to the soil as chemical fertilizer is immobilized rapidly and becomes unavailable to plants. [2] Currently, the main purpose in managing soil phosphorus is to optimize crop production and minimize P loss from soils. PSB have attracted the attention of agriculturists as soil inoculums to improve the plant growth and yield. When PSB is used with rock phosphate, it can save about 50% of the crop requirement of phosphatic fertilizer.[ citation needed ] The use of PSB as inoculants increases P uptake by plants. Simple inoculation of seeds with PSB gives crop yield responses equivalent to 30 kg P2O5 /ha or 50 percent of the need for phosphatic fertilizers. Alternatively, PSB can be applied through fertigation or in hydroponic operations. Many different strains of these bacteria have been identified as PSB, including Pantoea agglomerans (P5), Microbacterium laevaniformans (P7) and Pseudomonas putida (P13) strains are highly efficient insoluble phosphate solubilizers. Recently, researchers at Colorado State University demonstrated that a consortium of four bacteria, synergistically solubilize phosphorus at a much faster rate than any single strain alone. [3] Mahamuni and Patil (2012) isolated four strains of phosphate solubilizing bacteria from sugarcane (VIMP01 and VIMP02) and sugar beet rhizosphere (VIMP03 and VIMP 04). Isolates were strains of Burkholderia named as VIMP01, VIMP02, VIMP03 and VIMP04. VIMP (Vasantdada Sugar Institute Isolate by Mahamuni and Patil) cultures were identified as Burkholderia cenocepacia strain VIMP01 (JQ867371), Burkholderia gladioli strain VIMP02 (JQ811557), Burkholderia gladioli strain VIMP03 (JQ867372) and Burkholderia species strain VIMP04 (JQ867373). [4]

Additionally, phosphate (P) compounds are capable of immobilizing heavy metals, especially Pb, in contaminated environments through phosphate-heavy metal precipitation. However, most P compounds are not readily soluble in soils so it is not readily used for metal immobilization. Phosphate solubilizing bacteria (PSB) have the potential to enhance phosphate-induced immobilization of metals to remediate contaminated soil. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. [5]

Phosphate is often adsorbed onto the surface of different type of minerals, for example iron containing minerals. Recent data suggest that bacteria growing under phosphorus starvation release iron-chelating molecules. Considering the geochemical interaction between these two elements, the authors suggest that some bacteria can dissolve iron-containing minerals in order to access the adsorbed phosphate. [6]

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<span class="mw-page-title-main">Phosphate</span> Chemical compound

In chemistry, a phosphate is an anion, salt, functional group or ester derived from a phosphoric acid. It most commonly means orthophosphate, a derivative of orthophosphoric acid, a.k.a. phosphoric acid H3PO4.

<span class="mw-page-title-main">Fertilizer</span> Substance added to soils to supply plant nutrients for a better growth

A fertilizer or fertiliser is any material of natural or synthetic origin that is applied to soil or to plant tissues to supply plant nutrients. Fertilizers may be distinct from liming materials or other non-nutrient soil amendments. Many sources of fertilizer exist, both natural and industrially produced. For most modern agricultural practices, fertilization focuses on three main macro nutrients: nitrogen (N), phosphorus (P), and potassium (K) with occasional addition of supplements like rock flour for micronutrients. Farmers apply these fertilizers in a variety of ways: through dry or pelletized or liquid application processes, using large agricultural equipment or hand-tool methods.

<span class="mw-page-title-main">Iron(III)</span> The element iron in its +3 oxidation state

In chemistry, iron (III) refers to the element iron in its +3 oxidation state. In ionic compounds (salts), such an atom may occur as a separate cation (positive ion) denoted by Fe3+.

<span class="mw-page-title-main">Plant nutrition</span> Study of the chemical elements and compounds necessary for normal plant life

Plant nutrition is the study of the chemical elements and compounds necessary for plant growth and reproduction, plant metabolism and their external supply. In its absence the plant is unable to complete a normal life cycle, or that the element is part of some essential plant constituent or metabolite. This is in accordance with Justus von Liebig’s law of the minimum. The total essential plant nutrients include seventeen different elements: carbon, oxygen and hydrogen which are absorbed from the air, whereas other nutrients including nitrogen are typically obtained from the soil.

<span class="mw-page-title-main">Siderophore</span> Iron compounds secreted by microorganisms

Siderophores (Greek: "iron carrier") are small, high-affinity iron-chelating compounds that are secreted by microorganisms such as bacteria and fungi. They help the organism accumulate iron. Although a widening range of siderophore functions is now being appreciated, siderophores are among the strongest (highest affinity) Fe3+ binding agents known. Phytosiderophores are siderophores produced by plants.

<span class="mw-page-title-main">Soil fertility</span> The ability of a soil to sustain agricultural plant growth

Soil fertility refers to the ability of soil to sustain agricultural plant growth, i.e. to provide plant habitat and result in sustained and consistent yields of high quality. It also refers to the soil's ability to supply plant/crop nutrients in the right quantities and qualities over a sustained period of time. A fertile soil has the following properties:

<span class="mw-page-title-main">Phosphonate</span> Organic compound containing C–PO(OR)2 groups

In organic chemistry, phosphonates or phosphonic acids are organophosphorus compounds containing C−PO(OR)2 groups. Phosphonic acids, typically handled as salts, are generally nonvolatile solids that are poorly soluble in organic solvents, but soluble in water and common alcohols.

Agrogeology is the study of the origins of minerals known as agrominerals and their applications. These minerals are of importance to farming and horticulture, especially with regard to soil fertility and fertilizer components. These minerals are usually essential plant nutrients. Agrogeology can also be defined as the application of geology to problems in agriculture, particularly in reference to soil productivity and health. This field is a combination of a few different fields, including geology, soil science, agronomy, and chemistry. The overall objective is to advance agricultural production by using geological resources to improve chemical and physical aspects of soil.

<i>Burkholderia cenocepacia</i> Species of bacterium

Burkholderia cenocepacia is a Gram-negative, rod-shaped bacterium that is commonly found in soil and water environments and may also be associated with plants and animals, particularly as a human pathogen. It is one of over 20 species in the Burkholderia cepacia complex (Bcc) and is notable due to its virulence factors and inherent antibiotic resistance that render it a prominent opportunistic pathogen responsible for life-threatening, nosocomial infections in immunocompromised patients, such as those with cystic fibrosis or chronic granulomatous disease. The quorum sensing systems CepIR and CciIR regulate the formation of biofilms and the expression of virulence factors such as siderophores and proteases. Burkholderia cenocepacia may also cause disease in plants, such as in onions and bananas. Additionally, some strains serve as plant growth-promoting rhizobacteria.

<span class="mw-page-title-main">Phosphorus cycle</span> Biogeochemical movement

The phosphorus cycle is the biogeochemical cycle that describes the movement of phosphorus through the lithosphere, hydrosphere, and biosphere. Unlike many other biogeochemical cycles, the atmosphere does not play a significant role in the movement of phosphorus, because phosphorus and phosphorus-based compounds are usually solids at the typical ranges of temperature and pressure found on Earth. The production of phosphine gas occurs in only specialized, local conditions. Therefore, the phosphorus cycle should be viewed from whole Earth system and then specifically focused on the cycle in terrestrial and aquatic systems.

<span class="mw-page-title-main">Rhizobacteria</span> Group of bacteria affecting plant growth

Rhizobacteria are root-associated bacteria that can have a detrimental, neutral or beneficial effect on plant growth. The name comes from the Greek rhiza, meaning root. The term usually refers to bacteria that form symbiotic relationships with many plants (mutualism). Rhizobacteria are often referred to as plant growth-promoting rhizobacteria, or PGPRs. The term PGPRs was first used by Joseph W. Kloepper in the late 1970s and has become commonly used in scientific literature.

Microbial inoculants also known as soil inoculants or bioinoculants are agricultural amendments that use beneficial rhizosphericic or endophytic microbes to promote plant health. Many of the microbes involved form symbiotic relationships with the target crops where both parties benefit (mutualism). While microbial inoculants are applied to improve plant nutrition, they can also be used to promote plant growth by stimulating plant hormone production. Although bacterial and fungal inoculants are common, inoculation with archaea to promote plant growth is being increasingly studied.

<span class="mw-page-title-main">Agricultural pollution</span> Type of pollution caused by agriculture

Agricultural pollution refers to biotic and abiotic byproducts of farming practices that result in contamination or degradation of the environment and surrounding ecosystems, and/or cause injury to humans and their economic interests. The pollution may come from a variety of sources, ranging from point source water pollution to more diffuse, landscape-level causes, also known as non-point source pollution and air pollution. Once in the environment these pollutants can have both direct effects in surrounding ecosystems, i.e. killing local wildlife or contaminating drinking water, and downstream effects such as dead zones caused by agricultural runoff is concentrated in large water bodies.

<span class="mw-page-title-main">Biofertilizer</span> Substance with micro-organisms

A biofertilizer is a substance which contains living micro-organisms which, when applied to seeds, plant surfaces, or soil, colonize the rhizosphere or the interior of the plant and promotes growth by increasing the supply or availability of primary nutrients to the host plant. Biofertilizers add nutrients through the natural processes of nitrogen fixation, solubilizing phosphorus, and stimulating plant growth through the synthesis of growth-promoting substances. The micro-organisms in biofertilizers restore the soil's natural nutrient cycle and build soil organic matter. Through the use of biofertilizers, healthy plants can be grown, while enhancing the sustainability and the health of the soil. Biofertilizers can be expected to reduce the use of synthetic fertilizers and pesticides, but they are not yet able to replace their use. Since they play several roles, a preferred scientific term for such beneficial bacteria is "plant-growth promoting rhizobacteria" (PGPR).

Phosphate rich organic manure is a type of fertilizer used as an alternative to diammonium phosphate and single super phosphate.

Agricultural microbiology is a branch of microbiology dealing with plant-associated microbes and plant and animal diseases. It also deals with the microbiology of soil fertility, such as microbial degradation of organic matter and soil nutrient transformations.

A bioeffector is a viable microorganism or active natural compound which directly or indirectly affects plant performance (biofertilizer), and thus has the potential to reduce fertilizer and pesticide use in crop production.

<span class="mw-page-title-main">Bioremediation of radioactive waste</span>

Bioremediation of radioactive waste or bioremediation of radionuclides is an application of bioremediation based on the use of biological agents bacteria, plants and fungi to catalyze chemical reactions that allow the decontamination of sites affected by radionuclides. These radioactive particles are by-products generated as a result of activities related to nuclear energy and constitute a pollution and a radiotoxicity problem due to its unstable nature of ionizing radiation emissions.

<span class="mw-page-title-main">Mycorrhiza helper bacteria</span> Group of organisms

Mycorrhiza helper bacteria (MHB) are a group of organisms that form symbiotic associations with both ectomycorrhiza and arbuscular mycorrhiza. MHBs are diverse and belong to a wide variety of bacterial phyla including both Gram-negative and Gram-positive bacteria. Some of the most common MHBs observed in studies belong to the phylas Pseudomonas and Streptomyces. MHBs have been seen to have extremely specific interactions with their fungal hosts at times, but this specificity is lost with plants. MHBs enhance mycorrhizal function, growth, nutrient uptake to the fungus and plant, improve soil conductance, aid against certain pathogens, and help promote defense mechanisms. These bacteria are naturally present in the soil, and form these complex interactions with fungi as plant root development starts to take shape. The mechanisms through which these interactions take shape are not well-understood and needs further study.

Seventeen elements or nutrients are essential for plant growth and reproduction. They are carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus (P), potassium (K), sulfur (S), calcium (Ca), magnesium (Mg), iron (Fe), boron (B), manganese (Mn), copper (Cu), zinc (Zn), molybdenum (Mo), nickel (Ni) and chlorine (Cl). Nutrients required for plants to complete their life cycle are considered essential nutrients. Nutrients that enhance the growth of plants but are not necessary to complete the plant's life cycle are considered non-essential, although some of them, such as silicon (Si), have been shown to improve nutrent availability, hence the use of stinging nettle and horsetail macerations in Biodynamic agriculture. With the exception of carbon, hydrogen and oxygen, which are supplied by carbon dioxide and water, and nitrogen, provided through nitrogen fixation, the nutrients derive originally from the mineral component of the soil. The Law of the Minimum expresses that when the available form of a nutrient is not in enough proportion in the soil solution, then other nutrients cannot be taken up at an optimum rate by a plant. A particular nutrient ratio of the soil solution is thus mandatory for optimizing plant growth, a value which might differ from nutrient ratios calculated from plant composition.

References

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  2. Mohammad Ali Malboobi; Parviz Owlia; Mandana Behbahani; Elaheh Sarokhani; Sara Moradi; Bagher Yakhchali; Ali Deljou; Kambiz Morabbi Heravi (2009). "Solubilization of organic and inorganic phosphates by three highly efficient soil bacterial isolates". World Journal of Microbiology and Biotechnology. 25 (8): 1471–1477. doi:10.1007/s11274-009-0037-z. S2CID   55724070.
  3. Baas, Peter; Bell, Colin; Mancini, Lauren M.; Lee, Melanie N.; Conant, Richard T.; Wallenstein, Matthew D. (2016-06-14). "Phosphorus mobilizing consortium Mammoth P™enhances plant growth". PeerJ. 4: e2121. doi: 10.7717/peerj.2121 . ISSN   2167-8359. PMC   4911952 . PMID   27326379.
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  5. Park, J. H., Bolan, N., Megharaj, M., & Naidu, R. (2011). Isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. Journal of hazardous materials, 185(2), 829-836.
  6. Romano S, Bondarev V, Kölling M, Dittmar T, Schulz-Vogt HN (2017). "Phosphate limitation triggers the dissolution of precipitated iron by the marine bacterium Pseudovibrio sp. FO-BEG1". Frontiers in Microbiology . 8 (364): 364. doi: 10.3389/fmicb.2017.00364 . PMC   5348524 . PMID   28352252.