TSBP1

TSBP1
Identifiers
Aliases	TSBP1 , TSBP, chromosome 6 open reading frame 10, testis expressed basic protein 1, C6orf10
External IDs	OMIM: 618151; HomoloGene: 81753; GeneCards: TSBP1; OMA:TSBP1 - orthologs
Gene location (Human) Chr. Chromosome 6 (human) [1] Band 6p21.32 Start 32,288,526 bp [1] End 32,371,912 bp [1]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left testis right testis testicle gonad sural nerve lymph node Achilles tendon tonsil cervix right ovary n/a More reference expression data BioGPS More reference expression data
Orthologs Species Human Mouse Entrez 10665 n/a Ensembl ENSG00000206245 ENSG00000236672 ENSG00000204296 ENSG00000206310 ENSG00000234280 ENSG00000237881 ENSG00000232106 ENSG00000226892 n/a UniProt Q5SRN2 n/a RefSeq (mRNA) NM_001286474 NM_001286475 NM_006781 n/a RefSeq (protein) NP_001273403 NP_001273404 NP_006772 n/a Location (UCSC) Chr 6: 32.29 – 32.37 Mb n/a PubMed search [2] n/a
Wikidata
View/Edit Human

TSBP1 is a protein that in humans is encoded by the TSBP1 gene. ^{[3] [4]} TSBP1 was previously known as C6orf10. C6orf10 is an open reading frame on chromosome 6 containing a protein that is ubiquitously expressed at low levels in the adult genome and may play a role during fetal development. C6orf10 has been found to be linked to both neurodegenerative and autoimmune diseases in adults. Expression of this gene is highest in the testis but is also seen in other tissue types such as the brain, lens of the eye and the medulla. ^{[5] [6] [7]}

mRNA Transcript

Multiple sequence alignment of all C6orf10 human isoforms. This image was generated using Clustal W and M view.

C6orf10 contains seven human mRNA splice variants (a, b, c, X1, X2, X3, X4).

Conserved non-coding region

TSBP1 contains a highly conserved stem loop structure in the 3' UTR from bases 100–124.

Highly conserved stem loop structure from C6orf10 isoform a

Protein

Composition

C6orf10 isoform a is rich in lysine (K), Glutamine (Q) and Glutamic acid (E) and poor in Histidine (H) and Phenylalanine(F) ^{[11] ]}. Isoform a is a basic protein with an isoelectric point of 9 and a molecular weight of 62,000 kDa. ^[12]

This isoform contains two transmembrane regions near the beginning of the amino acid sequence. ^[13] The first transmembrane region spans from residue 6 to residue 25 (19 total residues) and has an isoelectric point of 5. The second transmembrane region spans from residue 100 to residue 119 (19 total residues) and has an isoelectric point of 8. Isoform a contains a PTZ00121 domain ^[14] starting with residue 221 and going until the end of the protein. There are several repetitive sequences within this domain.

Secondary Structure

TSBP1 consists mostly of alpha helices and random coils. ^[15] There are only a few regions that contain beta sheets. ^{[16] [17]}

Subcellular Localization

TSBP1 is predicted to be localized to the Nucleus and the Endoplasmic Reticulum. ^[13] There is a signal peptide cleavage site between amino acid 30 and 31 ^[18] which includes the first transmembrane domain. This N-terminal region of C6orf10 is likely localized to the endoplasmic reticulum. The C-terminal region of the protein contain two nuclear localization signals from amino acid 489-505 and 513-529 indicating that the section of the protein after the signal peptide cleavage site is localized to the nucleus.

Expression

TSBP1 is ubiquitously expressed at low levels in the adult human genome. In adults, expression of this gene is highest in the testis. ^[19] C6orf10 is expressed at higher levels in fetal and embryonic tissues. This indicates C6orf10 may play a role in development.

Tissue Expression profile of C6orf10 gene in preimplanted embryos

C6orf10 Tissue Expression Profile for adults. Orange dots indicate a very low level of expression while the blue bar indicates high expression.

Regulation of expression

Transcriptional

TSBP1 has a promoter that is 1206 bases long. ^[20] This promoter overlaps with the 3' UTR but ends before the first codon. This promoter is fairly well conserved across primates except for a 136 nucleotide region midway through and the end of the promoter region. ^[21] Primates have insertions at these two regions that humans are missing. This may suggest that these regions of the promoter are not essential to humans.

Transcription factors

TSBP1 transcription is regulated by the binding of many transcription factors to the promoter region. The CCAAT binding protein and TATA box are highly conserved regions that are important in the initiation of transcription. Several of the transcription factors including EH1, NACA, NKX5-2, SIX4, VCR, etc. are involved in developmental pathways. ^[20]

Abbreviation	Transcription Factor Full Name	Matrix score	Strand
CSRNP-1	Cytosine-Serine rich nuclear protein 1(AXUD1, AXIN1 up-regulated-1)	1.0	+
CCAAT Box	CCAAT/enhancer binding protein (C/EBP), gamma	0.923	+
EH1	Engrailed Homeobox 1	0.862	+
Cart-1	Cartilage homeoprotein 1	0.997	-
ZFP 263	Zinc finger protein 263, ZKSCAN12 (Zinc finger protein with KRAB and SCAN domains 12)	0.921	+
SWI/SNF	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily, a member 3	0.999	-
TATA Box	Vertebrate TATA binding protein factor	0.899	+
HSF2	Heat shock Factor 2	0.974	-
Hmx2/Nkx5-2	Hmx2/Nkx5-2 homeodomain transcription factor	0.933	+
Pdx1	Insulin promoter factor 1; pancreatic and duodenal homeobox 1 (Pdx1)	0.924	+
LMX1A	LIM homeobox transcription factor 1 alpha	1.0	+
NACA	Nascent polypeptide-associated complex subunit alpha 1	1.0	-
Oct1	Octamer binding factor 1	0.921	+
POU6F1	POU class 6 homeobox 1	0.973	+
STAT 5B	Signal transducer and activator of transcription 5B	0.973	+
SIX 4	Sine oculis homeobox homolog 4	0.96	-
NMP4	Nuclear matrix protein 4	0.971	+
MSX	Homeodomain proteins MSX-1 and MSX-2	0.989	-
AREB6	Atp1a1 regulatory element binding factor 6	1.0	-
VCR	Vertebrate caudal related homeodomain protein	0.963	+

Protein Interactions

Most of the predicted protein interactions with C6orf10 are based solely on text mining and information gathered from genome-wide association studies. The two proteins with the highest interaction scores were Butyrophilin-like protein 2 (BTNL2) and Tetratricopeptide repeat domain containing TTC32. ^[22] BTNL2 is a negative regulator of T-cell activity and member of the immunoglobulin superfamily. BTNL2 is located in the C6orf10 gene neighborhood. TTC32 is from a protein family of structural repeat motifs that mediate protein-protein interactions in the formation of protein-protein complexes. ^[23] This may indicate the potential for C6orf10 to interact with another protein for form a complex.

Clinical significance

C6orf10 has been found to be associated with both neurodegenerative diseases and autoimmune diseases. These associations are mostly obtained from genome wide association studies. Common neurodegenerative diseases associated with C6orf10 include frontotemporal dementia, Parkinson's disease, ^[24] and Alzheimer's disease. ^[24] Autoimmune diseases associated with C6orf10 include Rheumatoid arthritis, ^{[25] [26] [24] [27]} psoriasis, ^{[28] [27]} multiple sclerosis, ^{[29] [24]} Grave's disease ^[24] and lupus.^{[ citation needed ]}

Homologs

Orthologs

By searching the NCBI BLAST database ^[30] for protein-protein interactions, it was found that C6orf10 is a protein only found in mammals. The BLAST database found the highest number of homologs in the Primates , Artiodactyla , and Carnivora . There were only a couple of homologs in the taxonomic orders of Rodentia, Chiroptera , and Perissodactyla . In the orders of Scandentia, Eulipotyphyla, Tubulidentata and sirenia there was only one complete homolog, but a few partial sequences do exist. There were partial protein sequences in Lagomorpha, Dermoptera , and Macroscelidea and there were no orthologs in Diprotodontia, Didelphimorphia, Cetacea, Dasyuromorphia, Pilosa, Monotremata, and Proboscidea . No homologs were found outside of mammals.

C6orf10 Isoform X4 Orthologs

	Latin Name	Common Name	Identity	Median Date of divergence (MYA)
Primates	Homo sapiens	Human	100%	0
Primates	Gorilla gorilla gorilla	Gorilla	83.36%	8.61
Primates	Pongo abelii	Sumatran Orangutan	82.81%	15.2
Carnivora	Canis lupus familiaris	Dog	49.79%	94
Carnivora	Canis lupus dingo	Australian Dog	49.33%	94
Artiodactyla	Bubalus bubalis	Water Buffalo	49.16%	94
Artiodactyla	Equus caballus	Horse	49.00%	94
Artiodactyla	Odocoileus virginianus texanus	White Tailed Deer	44.59%	94
Rodentia	Chinchilla lanigera	Chinchilla	41.99%	88
Rodentia	Mus caroli	Ryuku mouse	41.06%	88
Carnivora	Felis catus	Cat	40.77%	94
Rodentia	Rattus norvegicus	Brown Rat	37.45%	88

Paralogs

C6orf10 has one paralog that diverged about 135.6 million years ago. This paralog is called Thioredoxin domain containing protein 2 (TXNDC2). ^[30]

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[1] BLAST: Basic Local Alignment Search Tool. National Center for Biotechnology InformationAvailable at: [https://blast.ncbi.nlm.nih.gov/Blast.cgi nih.gov]. Accessdate 4 March 2019.

References

1. ENSG00000236672, ENSG00000204296, ENSG00000206310, ENSG00000234280, ENSG00000237881, ENSG00000232106, ENSG00000226892 GRCh38: Ensembl release 89: ENSG00000206245, ENSG00000236672, ENSG00000204296, ENSG00000206310, ENSG00000234280, ENSG00000237881, ENSG00000232106, ENSG00000226892 – Ensembl, May 2017
2. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
3. Stammers M, Rowen L, Rhodes D, Trowsdale J, Beck S (April 2000). "BTL-II: a polymorphic locus with homology to the butyrophilin gene family, located at the border of the major histocompatibility complex class II and class III regions in human and mouse". Immunogenetics. 51 (4–5): 373–82. doi:10.1007/s002510050633. PMID   10803852. S2CID   31938388.
4. "Entrez Gene: C6orf10 chromosome 6 open reading frame 10".
5. "AceView: Gene:C6orf10, a comprehensive annotation of human, mouse and worm genes with mRNAs or ESTsAceView". www.ncbi.nlm.nih.gov. Retrieved 25 February 2019.
6. "TSBP1 testis expressed basic protein 1 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 25 February 2019.
7. "Tissue expression of C6orf10 - Summary - The Human Protein Atlas". www.proteinatlas.org. Retrieved 25 February 2019.
8. "Clustal Omega < Multiple Sequence Alignment < EMBL-EBI". www.ebi.ac.uk. Retrieved 6 May 2019.
9. "MView < Multiple Sequence Alignment < EMBL-EBI". www.ebi.ac.uk. Retrieved 6 May 2019.
10. "The Mfold Web Server | mfold.rit.albany.edu". unafold.rna.albany.edu. Retrieved 5 May 2019.
11. "SAPS < Sequence Statistics < EMBL-EBI". www.ebi.ac.uk. Retrieved 6 May 2019.
12. "ExPASy - Compute pI/Mw tool". web.expasy.org. Retrieved 6 May 2019.
13. "PSORT II Prediction". psort.hgc.jp. Retrieved 6 May 2019.
14. "NCBI CDD CDD Conserved Protein Domain Neuromodulin_N". www.ncbi.nlm.nih.gov. Retrieved 6 May 2019.
15. "Coils output". embnet.vital-it.ch. Archived from the original on 6 May 2019. Retrieved 6 May 2019.
16. "CFSSP: Chou & Fasman Secondary Structure Prediction Server". www.biogem.org. Retrieved 6 May 2019.
17. "Archived". Archived from the original on 6 May 2019. Retrieved 30 May 2023.
18. "ProP 1.0 Server - prediction results". www.cbs.dtu.dk. Retrieved 6 May 2019.
19. "TSBP1 testis expressed basic protein 1 [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 6 May 2019.
20. "Genomatix - NGS Data Analysis & Personalized Medicine". www.genomatix.de. Retrieved 6 May 2019.
21. "Human BLAT Search". genome.ucsc.edu. Retrieved 6 May 2019.
22. "STRING: functional protein association networks". string-db.org. Retrieved 6 May 2019.
23. Zeytuni N, Zarivach R (March 2012). "Structural and functional discussion of the tetra-trico-peptide repeat, a protein interaction module". Structure. 20 (3): 397–405. doi: 10.1016/j.str.2012.01.006 . PMID   22404999.
24. Zhang M, Ferrari R, Tartaglia MC, Keith J, Surace EI, Wolf U, et al. (October 2018). "A C6orf10/LOC101929163 locus is associated with age of onset in C9orf72 carriers". Brain. 141 (10): 2895–2907. doi:10.1093/brain/awy238. PMC   6158742 . PMID   30252044.
25. Verma A, Basile AO, Bradford Y, Kuivaniemi H, Tromp G, Carey D, Gerhard GS, Crowe JE, Ritchie MD, Pendergrass SA (2016). "Phenome-Wide Association Study to Explore Relationships between Immune System Related Genetic Loci and Complex Traits and Diseases". PLOS ONE. 11 (8): e0160573. Bibcode:2016PLoSO..1160573V. doi: 10.1371/journal.pone.0160573 . PMC   4980020 . PMID   27508393.
26. Zheng W, Rao S (August 2015). "Knowledge-based analysis of genetic associations of rheumatoid arthritis to inform studies searching for pleiotropic genes: a literature review and network analysis". Arthritis Research & Therapy. 17 (1): 202. doi: 10.1186/s13075-015-0715-1 . PMC   4529690 . PMID   26253105.
27. Malavia TA, Chaparala S, Wood J, Chowdari K, Prasad KM, McClain L, Jegga AG, Ganapathiraju MK, Nimgaonkar VL (2017). "Generating testable hypotheses for schizophrenia and rheumatoid arthritis pathogenesis by integrating epidemiological, genomic, and protein interaction data". npj Schizophrenia. 3: 11. doi:10.1038/s41537-017-0010-z. PMC   5441529 . PMID   28560257.
28. Feng BJ, Sun LD, Soltani-Arabshahi R, Bowcock AM, Nair RP, Stuart P, Elder JT, Schrodi SJ, Begovich AB, Abecasis GR, Zhang XJ, Callis-Duffin KP, Krueger GG, Goldgar DE (August 2009). "Multiple Loci within the major histocompatibility complex confer risk of psoriasis". PLOS Genetics. 5 (8): e1000606. doi: 10.1371/journal.pgen.1000606 . PMC   2718700 . PMID   19680446.
29. Lin X, Deng FY, Mo XB, Wu LF, Lei SF (January 2015). "Functional relevance for multiple sclerosis-associated genetic variants". Immunogenetics. 67 (1): 7–14. doi:10.1007/s00251-014-0803-4. PMID   25308886. S2CID   16113524.
30. "BLAST: Basic Local Alignment Search Tool". blast.ncbi.nlm.nih.gov. Retrieved 6 May 2019.

External links

• Human C6orf10 genome location and C6orf10 gene details page in the UCSC Genome Browser .

Further reading

• Ficarro S, Chertihin O, Westbrook VA, White F, Jayes F, Kalab P, Marto JA, Shabanowitz J, Herr JC, Hunt DF, Visconti PE (March 2003). "Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation". The Journal of Biological Chemistry. 278 (13): 11579–89. doi: 10.1074/jbc.M202325200 . PMID   12509440.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.