24-Dehydrocholesterol reductase

DHCR24
Identifiers
Aliases	DHCR24 , DCE, Nbla03646, SELADIN1, seladin-1, 24-dehydrocholesterol reductase
External IDs	OMIM: 606418 MGI: 1922004 HomoloGene: 8850 GeneCards: DHCR24
EC number	1.3.1.72
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p32.3 Start 54,849,627 bp [1] End 54,887,195 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4|4 C7 Start 106,418,235 bp [2] End 106,446,310 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in left adrenal gland right lobe of liver lower lobe of lung vulva nipple pons skin of abdomen gums bronchial epithelial cell corpus callosum Top expressed in lip yolk sac intestinal villus left lobe of liver skin of abdomen epidermis hair follicle crypt of lieberkuhn of small intestine sciatic nerve skin of back More reference expression data BioGPS More reference expression data
Gene ontology Molecular function peptide antigen binding flavin adenine dinucleotide binding oxidoreductase activity, acting on CH-OH group of donors delta24(24-1) sterol reductase activity oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor enzyme binding oxidoreductase activity delta24-sterol reductase activity FAD binding Cellular component cytoplasm integral component of membrane cytosol Golgi apparatus endoplasmic reticulum membrane membrane Golgi membrane endoplasmic reticulum cytoskeleton nucleus Biological process steroid biosynthetic process protein localization steroid metabolic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process male genitalia development sterol biosynthetic process lipid metabolism negative regulation of apoptotic process cholesterol metabolic process response to oxidative stress regulation of neuron death tissue development cholesterol biosynthetic process via lathosterol response to hormone sterol metabolic process membrane organization Ras protein signal transduction plasminogen activation cholesterol biosynthetic process via desmosterol amyloid precursor protein catabolic process negative regulation of cell population proliferation skin development apoptotic process cholesterol biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1718 74754 Ensembl ENSG00000116133 ENSMUSG00000034926 UniProt Q15392 Q8VCH6 RefSeq (mRNA) NM_014762 NM_053272 RefSeq (protein) NP_055577 NP_444502 Location (UCSC) Chr 1: 54.85 – 54.89 Mb Chr 4: 106.42 – 106.45 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

24-Dehydrocholesterol reductase is a protein that in humans is encoded by the DHCR24 gene. ^{[5] [6]}

This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase, which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. ^[6]

Model organisms

Dhcr24 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Abnormal
Recessive lethal study	Abnormal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Plasma immunoglobulins	Normal
Haematology	Normal
Peripheral blood lymphocytes	Normal
Micronucleus test	Normal
Heart weight	Normal
Skin Histopathology	Normal
Brain histopathology	Normal
Salmonella infection	Normal [7]
Citrobacter infection	Normal [8]
All tests and analysis from [9] [10]

Model organisms have been used in the study of DHCR24 function. A conditional knockout mouse line, called Dhcr24^{tm1a(EUCOMM)Wtsi [11] [12]} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists. ^{[13] [14] [15]}

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. ^{[9] [16]} Twenty-six tests were carried out on mutant mice and two significant abnormalities were observed. ^[9] Few homozygous mutant embryos were identified during gestation, and none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice and no further abnormalities were observed. ^[9]

See also

• Steroidogenic enzyme

References

1. GRCh38: Ensembl release 89: ENSG00000116133 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000034926 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Waterham HR, Koster J, Romeijn GJ, Hennekam RC, Vreken P, Andersson HC, FitzPatrick DR, Kelley RI, Wanders RJ (Sep 2001). "Mutations in the 3beta-hydroxysterol Delta24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesis". Am J Hum Genet. 69 (4): 685–94. doi:10.1086/323473. PMC   1226055 . PMID   11519011.
6. "Entrez Gene: DHCR24 24-dehydrocholesterol reductase".
7. "Salmonella infection data for Dhcr24". Wellcome Trust Sanger Institute.
8. "Citrobacter infection data for Dhcr24". Wellcome Trust Sanger Institute.
9. White JK, Gerdin AK, Karp NA, et al. (July 2013). "Genome-wide Generation and Systematic Phenotyping of Knockout Mice Reveals New Roles for Many Genes". Cell. 154 (2): 452–64. doi:10.1016/j.cell.2013.06.022. PMC   3717207 . PMID   23870131.
10. Mouse Resources Portal, Wellcome Trust Sanger Institute.
11. "International Knockout Mouse Consortium".
12. "Mouse Genome Informatics".
13. Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature. 474 (7351): 337–342. doi:10.1038/nature10163. PMC   3572410 . PMID   21677750.
14. Dolgin E (2011). "Mouse library set to be knockout". Nature. 474 (7351): 262–3. doi: 10.1038/474262a . PMID   21677718.
15. Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell. 128 (1): 9–13. doi: 10.1016/j.cell.2006.12.018 . PMID   17218247. S2CID   18872015.
16. van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism". Genome Biol. 12 (6): 224. doi: 10.1186/gb-2011-12-6-224 . PMC   3218837 . PMID   21722353.

External links

• Human DHCR24 genome location and DHCR24 gene details page in the UCSC Genome Browser .

Further reading

• Peri A, Danza G, Serio M (2005). "Seladin-1 as a target of estrogen receptor activation in the brain: a new gene for a rather old story?". J. Endocrinol. Invest. 28 (3): 285–93. doi:10.1007/bf03345387. hdl: 2158/308964 . PMID   15954227. S2CID   36435879.
• Nomura N, Miyajima N, Sazuka T, et al. (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1". DNA Res. 1 (1): 27–35. doi: 10.1093/dnares/1.1.27 . PMID   7584026.
• Nomura N, Miyajima N, Sazuka T, et al. (1995). "Prediction of the coding sequences of unidentified human genes. I. The coding sequences of 40 new genes (KIAA0001-KIAA0040) deduced by analysis of randomly sampled cDNA clones from human immature myeloid cell line KG-1 (supplement)". DNA Res. 1 (1): 47–56. doi: 10.1093/dnares/1.1.47 . PMID   7584028.
• Greeve I, Hermans-Borgmeyer I, Brellinger C, et al. (2001). "The human DIMINUTO/DWARF1 homolog seladin-1 confers resistance to Alzheimer's disease-associated neurodegeneration and oxidative stress". J. Neurosci. 20 (19): 7345–52. doi:10.1523/JNEUROSCI.20-19-07345.2000. PMC   6772756 . PMID   11007892.
• Andersson HC, Kratz L, Kelley R (2003). "Desmosterolosis presenting with multiple congenital anomalies and profound developmental delay". Am. J. Med. Genet. 113 (4): 315–9. doi:10.1002/ajmg.b.10873. PMID   12457401.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Luciani P, Ferruzzi P, Arnaldi G, et al. (2004). "Expression of the novel adrenocorticotropin-responsive gene selective Alzheimer's disease indicator-1 in the normal adrenal cortex and in adrenocortical adenomas and carcinomas". J. Clin. Endocrinol. Metab. 89 (3): 1332–9. doi: 10.1210/jc.2003-031065 . PMID   15001630.
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC   515316 . PMID   15342556.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Wu C, Miloslavskaya I, Demontis S, et al. (2005). "Regulation of cellular response to oncogenic and oxidative stress by Seladin-1". Nature. 432 (7017): 640–5. doi: 10.1038/nature03173 . PMID   15577914.
• Di Stasi D, Vallacchi V, Campi V, et al. (2005). "DHCR24 gene expression is upregulated in melanoma metastases and associated to resistance to oxidative stress-induced apoptosis". Int. J. Cancer. 115 (2): 224–30. doi: 10.1002/ijc.20885 . PMID   15688385.
• Crameri A, Biondi E, Kuehnle K, et al. (2006). "The role of seladin-1/DHCR24 in cholesterol biosynthesis, APP processing and Abeta generation in vivo". EMBO J. 25 (2): 432–43. doi:10.1038/sj.emboj.7600938. PMC   1383521 . PMID   16407971.
• Benvenuti S, Saccardi R, Luciani P, et al. (2006). "Neuronal differentiation of human mesenchymal stem cells: changes in the expression of the Alzheimer's disease-related gene seladin-1". Exp. Cell Res. 312 (13): 2592–604. doi:10.1016/j.yexcr.2006.04.016. hdl: 2158/310641 . PMID   16762343. S2CID   22809689.
• Lämsä R, Helisalmi S, Hiltunen M, et al. (2007). "The association study between DHCR24 polymorphisms and Alzheimer's disease". Am. J. Med. Genet. B Neuropsychiatr. Genet. 144 (7): 906–10. doi:10.1002/ajmg.b.30532. PMID   17510943. S2CID   25107832.