Aldehyde-stabilized cryopreservation

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Aldehyde-stabilized cryopreservation is a new technique for cryopreservation first demonstrated in 2016 by Robert L. McIntyre and Gregory Fahy at the cryobiology research company 21st Century Medicine, Inc. This technique uses a particular implementation of fixation and vitrification that can successfully preserve a rabbit brain in "near perfect" condition at −135  °C, with the cell membranes, synapses, and intracellular structures intact in electron micrographs. [1] In 2016, McIntyre and Fahy were awarded the first portion of the Brain Preservation Technology Prize, the Small Animal Brain Preservation Prize, by the Brain Preservation Foundation for the successful cryopreservation of a whole mouse brain. [2] [3] The cryopreserved brain was rewarmed and no serious degradation was found to have occurred; the brain structure under electron microscopic evaluation after rewarming remained well-preserved. [4] [5] Although this technique has not yet lead to a successful revival of a cryopreserved brain, some researchers see this technique as providing promising directions for future research. [6]

See also

References

  1. ↑ McIntyre RL, Fahy GM (December 2015). "Aldehyde-stabilized cryopreservation". Cryobiology. 71 (3): 448–458. doi: 10.1016/j.cryobiol.2015.09.003 . PMID   26408851.
  2. ↑ Claire Maldarelli (9 February 2016). "Researchers Have Preserved an Entire Rabbit Brain". Popular Science . Archived from the original on 14 March 2016. Retrieved 20 March 2016.
  3. ↑ Michael Shermer (1 February 2016). "Can Our Minds Live Forever?". Scientific American . Archived from the original on 22 March 2016. Retrieved 20 March 2016.
  4. ↑ "21st Century Medicine's Aldehyde-Stabilized Cryopreservation". Brain Preservation Foundation. 16 September 2015.
  5. ↑ "Aldehyde-Stabilized Cryopreservation Wins Final Phase of Brain Preservation Prize". PRWeb.
  6. ↑ Ekpo, Marlene Davis; Boafo, George Frimpong; Gambo, Suleiman Shafiu; Hu, Yuying; Liu, Xiangjian; Xie, Jingxian; Tan, Songwen (2022-06-09). "Cryopreservation of Animals and Cryonics: Current Technical Progress, Difficulties and Possible Research Directions". Frontiers in Veterinary Science. 9 877163. Frontiers. doi: 10.3389/fvets.2022.877163 . PMC   9219731 . PMID   35754544.


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