Aliphatic (R)-hydroxynitrile lyase

Aliphatic (R)-hydroxynitrile lyase
Identifiers
EC no.	4.1.2.46
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Aliphatic (R)-hydroxynitrile lyase (EC 4.1.2.46, (R)-HNL, (R)-oxynitrilase, (R)-hydroxynitrile lyase, LuHNL) is an enzyme with systematic name (2R)-2-hydroxy-2-methylbutanenitrile butan-2-one-lyase (cyanide forming). ^{[1] [2] [3] [4] [5] [6]} This enzyme catalyses the following chemical reaction:

(2R)-2-hydroxy-2-methylbutanenitrile ${\displaystyle \rightleftharpoons }$ cyanide + butan-2-one

The enzyme contains Zn²⁺.

References

1. Trummler, K.; Roos, J.; Schwaneberg, U.; Effenberger, F.; Förster, S.; Pfizenmaier, K.; Wajant, H. (1998). "Expression of the Zn²⁺-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris— utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis". Plant Sci. 139: 19–27. doi:10.1016/s0168-9452(98)00173-3.
2. Trummler, K.; Wajant, H. (1997). "Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases". J. Biol. Chem. 272 (8): 4770–4774. doi: 10.1074/jbc.272.8.4770 . PMID   9030531.
3. Albrecht J, Jansen I, Kula MR (1993). "Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range". Biotechnol. Appl. Biochem. 17 (2): 191–203. doi:10.1111/j.1470-8744.1993.tb00239.x. PMID   8387315.
4. Xu, L.-L.; Singh, B.K.; Conn, E.E. (1988). "Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum". Arch. Biochem. Biophys. 263 (2): 256–263. doi:10.1016/0003-9861(88)90634-0. PMID   3377504.
5. Cabirol, F.L.; Tan, P.L.; Tay, B.; Cheng, S.; Hanefeld, U.; Sheldon, R.A. (2008). "Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst". Adv. Synth. Catal. 350 (14–15): 2329–2338. doi:10.1002/adsc.200800309.
6. Breithaupt, H.; Pohl, M.; Bönigk, W.; Heim, P.; Schimz, K.-L.; Kula, M.-R. (1999). "Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)". J. Mol. Catal. B. 6 (3): 315–332. doi:10.1016/S1381-1177(98)00109-X.

External links

• Aliphatic+(R)-hydroxynitrile+lyase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)