CDC27

CDC27
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3T1N, 4RG6, 4RG7, 4RG9, 4UI9, 5A31, 5G04, 5G05
Identifiers
Aliases	CDC27 , ANAPC3, APC3, CDC27Hs, D0S1430E, D17S978E, H-NUC, HNUC, NUC2, cell division cycle 27
External IDs	OMIM: 116946; MGI: 102685; HomoloGene: 960; GeneCards: CDC27; OMA:CDC27 - orthologs
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q21.32 Start 47,117,703 bp [1] End 47,189,422 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11 E1|11 67.79 cM Start 104,502,745 bp [2] End 104,550,620 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte buccal mucosa cell tibia Achilles tendon epithelium of colon Brodmann area 23 tendon of biceps brachii internal globus pallidus stromal cell of endometrium germinal epithelium Top expressed in genital tubercle tail of embryo zygote cumulus cell secondary oocyte mandibular prominence maxillary prominence renal corpuscle pineal gland human fetus More reference expression data BioGPS n/a
Gene ontology Molecular function protein phosphatase binding protein binding Cellular component cytoplasm cytosol centrosome spindle spindle microtubule anaphase-promoting complex nucleus nucleoplasm Biological process metaphase anaphase-promoting complex-dependent catabolic process cell population proliferation protein ubiquitination protein K11-linked ubiquitination regulation of mitotic cell cycle phase transition ubiquitin-dependent protein catabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 996 217232 Ensembl ENSG00000004897 ENSMUSG00000020687 UniProt P30260 A2A6Q5 RefSeq (mRNA) NM_001114091 NM_001256 NM_001293089 NM_001293091 NM_001353035 NM_001353047 NM_001353049 NM_001353050 NM_001353051 NM_001285988 NM_001285989 NM_001285990 NM_145436 RefSeq (protein) NP_001107563 NP_001247 NP_001280018 NP_001280020 NP_001339964 NP_001339976 NP_001339978 NP_001339979 NP_001339980 NP_001272917 NP_001272918 NP_001272919 NP_663411 Location (UCSC) Chr 17: 47.12 – 47.19 Mb Chr 11: 104.5 – 104.55 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. ^{[5] [6]}

Function

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. ^[6]

Interactions

CDC27 has been shown to interact with:

• ANAPC10, ^{[7] [8]}
• ANAPC11, ^{[7] [8]}
• ANAPC1, ^{[7] [9]}
• ANAPC4, ^{[7] [8]}
• ANAPC5, ^{[7] [8] [10]}
• ANAPC7, ^{[7] [8]}
• CDC16, ^{[7] [8] [11] [12]}
• CDC20, ^{[7] [11] [13] [14] [15] [16] [17]}
• CDC23, ^{[7] [8]}
• CDH1, ^[7]
• FZR1, ^{[13] [18]}
• MAD2L1 ^{[11] [16]} and
• PIN1. ^{[19] [20]}

References

1. GRCh38: Ensembl release 89: ENSG00000004897 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020687 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Tugendreich S, Boguski MS, Seldin MS, Hieter P (Nov 1993). "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base". Proceedings of the National Academy of Sciences of the United States of America. 90 (21): 10031–5. Bibcode:1993PNAS...9010031T. doi: 10.1073/pnas.90.21.10031 . PMC   47707 . PMID   8234252.
6. "Entrez Gene: CDC27 cell division cycle 27 homolog (S. cerevisiae)".
7. Vodermaier HC, Gieffers C, Maurer-Stroh S, Eisenhaber F, Peters JM (Sep 2003). "TPR subunits of the anaphase-promoting complex mediate binding to the activator protein CDH1". Current Biology. 13 (17): 1459–68. Bibcode:2003CBio...13.1459V. doi: 10.1016/s0960-9822(03)00581-5 . PMID   12956947. S2CID   5942532.
8. Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM (Aug 2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (16): 8973–8. Bibcode:2000PNAS...97.8973G. doi: 10.1073/pnas.97.16.8973 . PMC   16806 . PMID   10922056.
9. Sumara I, Vorlaufer E, Gieffers C, Peters BH, Peters JM (Nov 2000). "Characterization of vertebrate cohesin complexes and their regulation in prophase". The Journal of Cell Biology. 151 (4): 749–62. doi:10.1083/jcb.151.4.749. PMC   2169443 . PMID   11076961.
10. Koloteva-Levine N, Pinchasi D, Pereman I, Zur A, Brandeis M, Elroy-Stein O (May 2004). "The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation". Molecular and Cellular Biology. 24 (9): 3577–87. doi:10.1128/mcb.24.9.3577-3587.2004. PMC   387753 . PMID   15082755.
11. Kallio M, Weinstein J, Daum JR, Burke DJ, Gorbsky GJ (Jun 1998). "Mammalian p55CDC mediates association of the spindle checkpoint protein Mad2 with the cyclosome/anaphase-promoting complex, and is involved in regulating anaphase onset and late mitotic events". The Journal of Cell Biology. 141 (6): 1393–406. doi:10.1083/jcb.141.6.1393. PMC   2132789 . PMID   9628895.
12. Ollendorff V, Donoghue DJ (Dec 1997). "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex". The Journal of Biological Chemistry. 272 (51): 32011–8. doi: 10.1074/jbc.272.51.32011 . PMID   9405394.
13. Kramer ER, Gieffers C, Hölzl G, Hengstschläger M, Peters JM (Nov 1998). "Activation of the human anaphase-promoting complex by proteins of the CDC20/Fizzy family". Current Biology. 8 (22): 1207–10. Bibcode:1998CBio....8.1207K. doi: 10.1016/s0960-9822(07)00510-6 . PMID   9811605. S2CID   17181162.
14. D'Angiolella V, Mari C, Nocera D, Rametti L, Grieco D (Oct 2003). "The spindle checkpoint requires cyclin-dependent kinase activity". Genes & Development. 17 (20): 2520–5. doi:10.1101/gad.267603. PMC   218146 . PMID   14561775.
15. Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (Sep 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell Biology. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC   2173153 . PMID   12196507.
16. Wassmann K, Benezra R (Sep 1998). "Mad2 transiently associates with an APC/p55Cdc complex during mitosis". Proceedings of the National Academy of Sciences of the United States of America. 95 (19): 11193–8. Bibcode:1998PNAS...9511193W. doi: 10.1073/pnas.95.19.11193 . PMC   21618 . PMID   9736712.
17. Nilsson J, Yekezare M, Minshull J, Pines J (Dec 2008). "The APC/C maintains the spindle assembly checkpoint by targeting Cdc20 for destruction". Nature Cell Biology. 10 (12): 1411–20. doi:10.1038/ncb1799. PMC   2635557 . PMID   18997788.
18. Zhou Y, Ching YP, Ng RW, Jin DY (Sep 2003). "Differential expression, localization and activity of two alternatively spliced isoforms of human APC regulator CDH1". The Biochemical Journal. 374 (Pt 2): 349–58. doi:10.1042/BJ20030600. PMC   1223613 . PMID   12797865.
19. Shen M, Stukenberg PT, Kirschner MW, Lu KP (Mar 1998). "The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins". Genes & Development. 12 (5): 706–20. doi:10.1101/gad.12.5.706. PMC   316589 . PMID   9499405.
20. Lu PJ, Zhou XZ, Shen M, Lu KP (Feb 1999). "Function of WW domains as phosphoserine- or phosphothreonine-binding modules". Science. 283 (5406): 1325–8. Bibcode:1999Sci...283.1325L. doi:10.1126/science.283.5406.1325. PMID   10037602.

Further reading

• Tugendreich S, Tomkiel J, Earnshaw W, Hieter P (Apr 1995). "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition". Cell. 81 (2): 261–8. doi: 10.1016/0092-8674(95)90336-4 . PMID   7736578. S2CID   17907584.
• Chen PL, Ueng YC, Durfee T, Chen KC, Yang-Feng T, Lee WH (Feb 1995). "Identification of a human homologue of yeast nuc2 which interacts with the retinoblastoma protein in a specific manner". Cell Growth & Differentiation. 6 (2): 199–210. PMID   7756179.
• Ho PP, Couch FJ, Brody LC, Abel KJ, Boehnke M, Shearon TH, Chandrasekharappa SC, Collins FS, Weber BL (Sep 1995). "Localization of the human homolog of the yeast cell division control 27 gene (CDC27) proximal to ITGB3 on human chromosome 17q21.3". Somatic Cell and Molecular Genetics. 21 (5): 351–5. doi:10.1007/BF02257470. hdl: 2027.42/45546 . PMID   8619132. S2CID   9614052.
• Ollendorff V, Donoghue DJ (Dec 1997). "The serine/threonine phosphatase PP5 interacts with CDC16 and CDC27, two tetratricopeptide repeat-containing subunits of the anaphase-promoting complex". The Journal of Biological Chemistry. 272 (51): 32011–8. doi: 10.1074/jbc.272.51.32011 . PMID   9405394.
• Yu H, Peters JM, King RW, Page AM, Hieter P, Kirschner MW (Feb 1998). "Identification of a cullin homology region in a subunit of the anaphase-promoting complex". Science. 279 (5354): 1219–22. Bibcode:1998Sci...279.1219Y. doi:10.1126/science.279.5354.1219. PMID   9469815.
• Shen M, Stukenberg PT, Kirschner MW, Lu KP (Mar 1998). "The essential mitotic peptidyl-prolyl isomerase Pin1 binds and regulates mitosis-specific phosphoproteins". Genes & Development. 12 (5): 706–20. doi:10.1101/gad.12.5.706. PMC   316589 . PMID   9499405.
• Zhou BB, Li H, Yuan J, Kirschner MW (Jun 1998). "Caspase-dependent activation of cyclin-dependent kinases during Fas-induced apoptosis in Jurkat cells". Proceedings of the National Academy of Sciences of the United States of America. 95 (12): 6785–90. Bibcode:1998PNAS...95.6785Z. doi: 10.1073/pnas.95.12.6785 . PMC   22635 . PMID   9618490.
• Wassmann K, Benezra R (Sep 1998). "Mad2 transiently associates with an APC/p55Cdc complex during mitosis". Proceedings of the National Academy of Sciences of the United States of America. 95 (19): 11193–8. Bibcode:1998PNAS...9511193W. doi: 10.1073/pnas.95.19.11193 . PMC   21618 . PMID   9736712.
• Lu PJ, Zhou XZ, Shen M, Lu KP (Feb 1999). "Function of WW domains as phosphoserine- or phosphothreonine-binding modules". Science. 283 (5406): 1325–8. Bibcode:1999Sci...283.1325L. doi:10.1126/science.283.5406.1325. PMID   10037602.
• Grossberger R, Gieffers C, Zachariae W, Podtelejnikov AV, Schleiffer A, Nasmyth K, Mann M, Peters JM (May 1999). "Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex". The Journal of Biological Chemistry. 274 (20): 14500–7. doi: 10.1074/jbc.274.20.14500 . PMID   10318877.
• Chan GK, Jablonski SA, Sudakin V, Hittle JC, Yen TJ (Sep 1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". The Journal of Cell Biology. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC   2169490 . PMID   10477750.
• Gieffers C, Peters BH, Kramer ER, Dotti CG, Peters JM (Sep 1999). "Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons". Proceedings of the National Academy of Sciences of the United States of America. 96 (20): 11317–22. Bibcode:1999PNAS...9611317G. doi: 10.1073/pnas.96.20.11317 . PMC   18031 . PMID   10500174.
• Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM (Aug 2000). "The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex". Proceedings of the National Academy of Sciences of the United States of America. 97 (16): 8973–8. Bibcode:2000PNAS...97.8973G. doi: 10.1073/pnas.97.16.8973 . PMC   16806 . PMID   10922056.
• Rippmann JF, Hobbie S, Daiber C, Guilliard B, Bauer M, Birk J, Nar H, Garin-Chesa P, Rettig WJ, Schnapp A (Jul 2000). "Phosphorylation-dependent proline isomerization catalyzed by Pin1 is essential for tumor cell survival and entry into mitosis". Cell Growth & Differentiation. 11 (7): 409–16. PMID   10939594.
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (Sep 2000). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Reports. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC   1083732 . PMID   11256614.
• Wendt KS, Vodermaier HC, Jacob U, Gieffers C, Gmachl M, Peters JM, Huber R, Sondermann P (Sep 2001). "Crystal structure of the APC10/DOC1 subunit of the human anaphase-promoting complex". Nature Structural Biology. 8 (9): 784–8. doi:10.1038/nsb0901-784. PMID   11524682. S2CID   27021761.
• Stroschein SL, Bonni S, Wrana JL, Luo K (Nov 2001). "Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN". Genes & Development. 15 (21): 2822–36. doi:10.1101/gad.912901. PMC   312804 . PMID   11691834.
• Kallio MJ, Beardmore VA, Weinstein J, Gorbsky GJ (Sep 2002). "Rapid microtubule-independent dynamics of Cdc20 at kinetochores and centrosomes in mammalian cells". The Journal of Cell Biology. 158 (5): 841–7. doi:10.1083/jcb.200201135. PMC   2173153 . PMID   12196507.
• Topper LM, Campbell MS, Tugendreich S, Daum JR, Burke DJ, Hieter P, Gorbsky GJ (2002). "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis". Cell Cycle. 1 (4): 282–92. doi:10.4161/cc.1.4.139. PMID   12429948. S2CID   10799434.

External links

• Human CDC27 genome location and CDC27 gene details page in the UCSC Genome Browser .
• Overview of all the structural information available in the PDB for UniProt : P30260 (Cell division cycle protein 27 homolog) at the PDBe-KB .