Geranylfarnesyl diphosphate synthase

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Geranylfarnesyl diphosphate synthase
Identifiers
EC no. 2.5.1.81
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BRENDA BRENDA entry
ExPASy NiceZyme view
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MetaCyc metabolic pathway
PRIAM profile
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NCBI proteins

Geranylfarnesyl diphosphate synthase (EC 2.5.1.81, FGPP synthase, (all-E) geranylfarnesyl diphosphate synthase, GFPS, Fgs) is an enzyme with systematic name geranylgeranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 1 isopentenyl unit). [1] [2] [3] [4] This enzyme catalyses the following chemical reaction

geranylgeranyl diphosphate + isopentenyl diphosphate (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate

The enzyme from Methanosarcina mazei is involved in biosynthesis of the polyprenyl side-chain of methanophenazine.

References

  1. Ogawa T, Yoshimura T, Hemmi H (February 2010). "Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution". Biochemical and Biophysical Research Communications. 393 (1): 16–20. Bibcode:2010BBRC..393...16O. doi:10.1016/j.bbrc.2010.01.063. PMID   20097171.
  2. Tachibana A, Yano Y, Otani S, Nomura N, Sako Y, Taniguchi M (January 2000). "Novel prenyltransferase gene encoding farnesylgeranyl diphosphate synthase from a hyperthermophilic archaeon, Aeropyrum pernix. Molecularevolution with alteration in product specificity". European Journal of Biochemistry. 267 (2): 321–8. doi: 10.1046/j.1432-1327.2000.00967.x . PMID   10632701.
  3. Tachibana A (March 1994). "A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis". FEBS Letters. 341 (2–3): 291–4. Bibcode:1994FEBSL.341..291T. doi:10.1016/0014-5793(94)80475-3. PMID   8137956.
  4. Lee PC, Mijts BN, Petri R, Watts KT, Schmidt-Dannert C (November 2004). "Alteration of product specificity of Aeropyrum pernix farnesylgeranyl diphosphate synthase (Fgs) by directed evolution". Protein Engineering, Design & Selection. 17 (11): 771–7. doi: 10.1093/protein/gzh089 . PMID   15548566.