PPP2CA

PPP2CA
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2IAE, 2IE3, 2IE4, 2NPP, 2NYL, 2NYM, 3C5W, 3DW8, 3FGA, 3K7V, 3K7W, 3P71, 4I5L, 4I5N, 4IYP, 4LAC
Identifiers
Aliases	PPP2CA , PP2Ac, PP2CA, PP2Calpha, RP-C, protein phosphatase 2 catalytic subunit alpha, NEDLBA
External IDs	OMIM: 176915 MGI: 1321159 HomoloGene: 37660 GeneCards: PPP2CA
Gene location (Human) Chr. Chromosome 5 (human) [1] Band 5q31.1 Start 134,194,332 bp [1] End 134,226,073 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11|11 B1.3 Start 51,989,508 bp [2] End 52,018,605 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ganglionic eminence stromal cell of endometrium postcentral gyrus prefrontal cortex pons right ventricle monocyte biceps brachii human penis superior frontal gyrus Top expressed in medial ganglionic eminence molar nucleus accumbens substantia nigra endocardial cushion barrel cortex atrioventricular valve abdominal wall lateral geniculate nucleus conjunctival fornix More reference expression data BioGPS More reference expression data
Gene ontology Molecular function phosphoprotein phosphatase activity GABA receptor binding metal ion binding protein C-terminus binding protein binding hydrolase activity protein heterodimerization activity protein serine/threonine phosphatase activity tau protein binding Cellular component cytoplasm cytosol spindle pole membrane microtubule cytoskeleton plasma membrane protein phosphatase type 2A complex chromosome mitochondrion chromosome, centromeric region cytoskeleton extracellular exosome nucleus membrane raft synapse Biological process apoptotic process regulation of transcription, DNA-templated ceramide metabolic process regulation of DNA replication protein dephosphorylation response to organic substance positive regulation of protein serine/threonine kinase activity regulation of Wnt signaling pathway regulation of cell adhesion meiosis negative regulation of cell growth RNA splicing regulation of cell differentiation regulation of growth mitotic nuclear membrane reassembly nuclear-transcribed mRNA catabolic process, nonsense-mediated decay mesoderm development second-messenger-mediated signaling negative regulation of epithelial to mesenchymal transition negative regulation of tyrosine phosphorylation of STAT protein regulation of protein phosphorylation response to lead ion peptidyl-threonine dephosphorylation peptidyl-serine dephosphorylation regulation of microtubule binding positive regulation of microtubule binding Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5515 19052 Ensembl ENSG00000113575 ENSMUSG00000020349 UniProt P67775 P63330 RefSeq (mRNA) NM_002715 NM_001355019 NM_019411 RefSeq (protein) NP_002706 NP_001341948 NP_062284 Location (UCSC) Chr 5: 134.19 – 134.23 Mb Chr 11: 51.99 – 52.02 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform is an enzyme that (in humans) is encoded by the PPP2CA gene. ^[5]

Function

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. ^[6]

Interactions

PPP2CA has been shown to interact with:

• Bcl-2, ^[7]
• Bestrophin 1, ^[8]
• CCNG2, ^[9]
• CTTNBP2NL, ^[10]
• CTTNBP2, ^[10]
• Cyclin-dependent kinase 2, ^[11]
• Cyclin-dependent kinase 6, ^[11]
• FAM40A, ^[10]
• IGBP1, ^{[10] [12] [13] [14]}
• MOBKL3, ^[10]
• PPP2R1A, ^{[10] [15]}
• PPP2R1B, ^{[10] [15]}
• PPP2R2A, ^{[10] [16]}
• PPP2R3B, ^{[10] [17]}
• PPP2R5A, ^{[10] [18]}
• PPP2R5B, ^{[10] [18]}
• PPP2R5C, ^{[10] [18] [19]}
• PPP2R5D, ^{[10] [18]}
• PPP2R5E, ^{[10] [18]}
• STRN3, ^[10]
• STRN, ^[10] and
• TLX1. ^[20]

See also

• PPP2CB

References

1. GRCh38: Ensembl release 89: ENSG00000113575 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020349 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT (April 1993). "Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23-->q31 and 8p12-->p11.2, respectively". Cytogenetics and Cell Genetics. 63 (1): 35–41. doi:10.1159/000133497. PMID   8383590.
6. "Entrez Gene: PPP2CA protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform".
7. Deng X, Ito T, Carr B, Mumby M, May WS (Dec 1998). "Reversible phosphorylation of Bcl2 following interleukin 3 or bryostatin 1 is mediated by direct interaction with protein phosphatase 2A". The Journal of Biological Chemistry. 273 (51): 34157–63. doi: 10.1074/jbc.273.51.34157 . PMID   9852076.
8. Marmorstein LY, McLaughlin PJ, Stanton JB, Yan L, Crabb JW, Marmorstein AD (Aug 2002). "Bestrophin interacts physically and functionally with protein phosphatase 2A". The Journal of Biological Chemistry. 277 (34): 30591–7. doi: 10.1074/jbc.M204269200 . PMID   12058047.
9. Bennin DA, Don AS, Brake T, McKenzie JL, Rosenbaum H, Ortiz L, DePaoli-Roach AA, Horne MC (Jul 2002). "Cyclin G2 associates with protein phosphatase 2A catalytic and regulatory B' subunits in active complexes and induces nuclear aberrations and a G1/S phase cell cycle arrest". The Journal of Biological Chemistry. 277 (30): 27449–67. doi: 10.1074/jbc.M111693200 . PMID   11956189.
10. Goudreault M, D'Ambrosio LM, Kean MJ, Mullin MJ, Larsen BG, Sanchez A, Chaudhry S, Chen GI, Sicheri F, Nesvizhskii AI, Aebersold R, Raught B, Gingras AC (Jan 2009). "A PP2A phosphatase high density interaction network identifies a novel striatin-interacting phosphatase and kinase complex linked to the cerebral cavernous malformation 3 (CCM3) protein". Molecular & Cellular Proteomics. 8 (1): 157–71. doi: 10.1074/mcp.M800266-MCP200 . PMC   2621004 . PMID   18782753.
11. Cheng A, Kaldis P, Solomon MJ (Nov 2000). "Dephosphorylation of human cyclin-dependent kinases by protein phosphatase type 2C alpha and beta 2 isoforms". The Journal of Biological Chemistry. 275 (44): 34744–9. doi: 10.1074/jbc.M006210200 . PMID   10934208.
12. Gingras AC, Caballero M, Zarske M, Sanchez A, Hazbun TR, Fields S, Sonenberg N, Hafen E, Raught B, Aebersold R (Nov 2005). "A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity". Molecular & Cellular Proteomics. 4 (11): 1725–40. doi: 10.1074/mcp.M500231-MCP200 . PMID   16085932.
13. Chen J, Peterson RT, Schreiber SL (Jun 1998). "Alpha 4 associates with protein phosphatases 2A, 4, and 6". Biochemical and Biophysical Research Communications. 247 (3): 827–32. doi:10.1006/bbrc.1998.8792. PMID   9647778.
14. Chung H, Nairn AC, Murata K, Brautigan DL (Aug 1999). "Mutation of Tyr307 and Leu309 in the protein phosphatase 2A catalytic subunit favors association with the alpha 4 subunit which promotes dephosphorylation of elongation factor-2". Biochemistry. 38 (32): 10371–6. doi:10.1021/bi990902g. PMID   10441131.
15. Zhou J, Pham HT, Ruediger R, Walter G (Jan 2003). "Characterization of the Aalpha and Abeta subunit isoforms of protein phosphatase 2A: differences in expression, subunit interaction, and evolution". The Biochemical Journal. 369 (Pt 2): 387–98. doi:10.1042/BJ20021244. PMC   1223084 . PMID   12370081.
16. Kamibayashi C, Lickteig RL, Estes R, Walter G, Mumby MC (Oct 1992). "Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits". The Journal of Biological Chemistry. 267 (30): 21864–72. doi: 10.1016/S0021-9258(19)36692-X . PMID   1328247.
17. Yan Z, Fedorov SA, Mumby MC, Williams RS (Feb 2000). "PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells". Molecular and Cellular Biology. 20 (3): 1021–9. doi:10.1128/mcb.20.3.1021-1029.2000. PMC   85219 . PMID   10629059.
18. McCright B, Rivers AM, Audlin S, Virshup DM (Sep 1996). "The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm". The Journal of Biological Chemistry. 271 (36): 22081–9. doi: 10.1074/jbc.271.36.22081 . PMID   8703017.
19. Ito A, Kataoka TR, Watanabe M, Nishiyama K, Mazaki Y, Sabe H, Kitamura Y, Nojima H (Feb 2000). "A truncated isoform of the PP2A B56 subunit promotes cell motility through paxillin phosphorylation". The EMBO Journal. 19 (4): 562–71. doi:10.1093/emboj/19.4.562. PMC   305594 . PMID   10675325.
20. Kawabe T, Muslin AJ, Korsmeyer SJ (Jan 1997). "HOX11 interacts with protein phosphatases PP2A and PP1 and disrupts a G2/M cell-cycle checkpoint". Nature. 385 (6615): 454–8. Bibcode:1997Natur.385..454K. doi:10.1038/385454a0. PMID   9009195. S2CID   608633.

Further reading

• Cohen P, Cohen PT (Dec 1989). "Protein phosphatases come of age". The Journal of Biological Chemistry. 264 (36): 21435–8. doi: 10.1016/S0021-9258(20)88197-6 . PMID   2557326.
• Zolnierowicz S (Oct 2000). "Type 2A protein phosphatase, the complex regulator of numerous signaling pathways". Biochemical Pharmacology. 60 (8): 1225–35. doi:10.1016/S0006-2952(00)00424-X. PMID   11007961.
• Kamibayashi C, Lickteig RL, Estes R, Walter G, Mumby MC (Oct 1992). "Expression of the A subunit of protein phosphatase 2A and characterization of its interactions with the catalytic and regulatory subunits". The Journal of Biological Chemistry. 267 (30): 21864–72. doi: 10.1016/S0021-9258(19)36692-X . PMID   1328247.
• Goedert M, Cohen ES, Jakes R, Cohen P (Nov 1992). "p42 MAP kinase phosphorylation sites in microtubule-associated protein tau are dephosphorylated by protein phosphatase 2A1. Implications for Alzheimer's disease [corrected]". FEBS Letters. 312 (1): 95–9. doi: 10.1016/0014-5793(92)81418-L . PMID   1330687. S2CID   34940651.
• Khew-Goodall Y, Mayer RE, Maurer F, Stone SR, Hemmings BA (Jan 1991). "Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes". Biochemistry. 30 (1): 89–97. doi:10.1021/bi00215a014. PMID   1846293.
• Scheidtmann KH, Virshup DM, Kelly TJ (Apr 1991). "Protein phosphatase 2A dephosphorylates simian virus 40 large T antigen specifically at residues involved in regulation of DNA-binding activity". Journal of Virology. 65 (4): 2098–101. doi:10.1128/JVI.65.4.2098-2101.1991. PMC   240073 . PMID   1848320.
• Scheidtmann KH, Mumby MC, Rundell K, Walter G (Apr 1991). "Dephosphorylation of simian virus 40 large-T antigen and p53 protein by protein phosphatase 2A: inhibition by small-t antigen". Molecular and Cellular Biology. 11 (4): 1996–2003. doi:10.1128/mcb.11.4.1996. PMC   359885 . PMID   1848668.
• Virshup DM, Kauffman MG, Kelly TJ (Dec 1989). "Activation of SV40 DNA replication in vitro by cellular protein phosphatase 2A". The EMBO Journal. 8 (12): 3891–8. doi:10.1002/j.1460-2075.1989.tb08568.x. PMC   402079 . PMID   2555176.
• Arino J, Woon CW, Brautigan DL, Miller TB, Johnson GL (Jun 1988). "Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes". Proceedings of the National Academy of Sciences of the United States of America. 85 (12): 4252–6. Bibcode:1988PNAS...85.4252A. doi: 10.1073/pnas.85.12.4252 . PMC   280405 . PMID   2837763.
• Stone SR, Mayer R, Wernet W, Maurer F, Hofsteenge J, Hemmings BA (Dec 1988). "The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A alpha catalytic subunit". Nucleic Acids Research. 16 (23): 11365. doi:10.1093/nar/16.23.11365. PMC   339016 . PMID   2849764.
• Carrey EA, Campbell DG, Hardie DG (Dec 1985). "Phosphorylation and activation of hamster carbamyl phosphate synthetase II by cAMP-dependent protein kinase. A novel mechanism for regulation of pyrimidine nucleotide biosynthesis". The EMBO Journal. 4 (13B): 3735–42. doi:10.1002/j.1460-2075.1985.tb04142.x. PMC   554725 . PMID   4092695.
• McCright B, Virshup DM (Nov 1995). "Identification of a new family of protein phosphatase 2A regulatory subunits". The Journal of Biological Chemistry. 270 (44): 26123–8. doi: 10.1074/jbc.270.44.26123 . PMID   7592815.
• Favre B, Zolnierowicz S, Turowski P, Hemmings BA (Jun 1994). "The catalytic subunit of protein phosphatase 2A is carboxyl-methylated in vivo". The Journal of Biological Chemistry. 269 (23): 16311–7. doi: 10.1016/S0021-9258(17)34009-7 . PMID   8206937.
• Redpath NT, Price NT, Severinov KV, Proud CG (Apr 1993). "Regulation of elongation factor-2 by multisite phosphorylation". European Journal of Biochemistry. 213 (2): 689–99. doi:10.1111/j.1432-1033.1993.tb17809.x. PMID   8386634.
• Muramatsu T, Kincaid RL (Jul 1993). "Molecular cloning of a full-length cDNA encoding the catalytic subunit of human calmodulin-dependent protein phosphatase (calcineurin A alpha)". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1178 (1): 117–20. doi:10.1016/0167-4889(93)90117-8. PMID   8392375.
• Kissinger CR, Parge HE, Knighton DR, Lewis CT, Pelletier LA, Tempczyk A, Kalish VJ, Tucker KD, Showalter RE, Moomaw EW (Dec 1995). "Crystal structures of human calcineurin and the human FKBP12-FK506-calcineurin complex". Nature. 378 (6557): 641–4. Bibcode:1995Natur.378..641K. doi:10.1038/378641a0. PMID   8524402. S2CID   4337105.
• Ohguro H, Rudnicka-Nawrot M, Buczyłko J, Zhao X, Taylor JA, Walsh KA, Palczewski K (Mar 1996). "Structural and enzymatic aspects of rhodopsin phosphorylation". The Journal of Biological Chemistry. 271 (9): 5215–24. doi: 10.1074/jbc.271.9.5215 . PMID   8617805.
• McCright B, Rivers AM, Audlin S, Virshup DM (Sep 1996). "The B56 family of protein phosphatase 2A (PP2A) regulatory subunits encodes differentiation-induced phosphoproteins that target PP2A to both nucleus and cytoplasm". The Journal of Biological Chemistry. 271 (36): 22081–9. doi: 10.1074/jbc.271.36.22081 . PMID   8703017.