Rhodococcus fascians

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Rhodococcus fascians
Rhodococcus fascians.jpg
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Actinomycetota
Class: Actinomycetia
Order: Mycobacteriales
Family: Nocardiaceae
Genus: Rhodococcus
Species:
R. fascians
Binomial name
Rhodococcus fascians
(Tilford 1936) Goodfellow 1984
Rhodococcus fascians on agar plate Rhodococcus fascians NRRL B-16937 (Type Strain).jpg
Rhodococcus fascians on agar plate

Rhodococcus fascians (known as Corynebacterium fascians until 1984) is a Gram positive bacterial phytopathogen that causes leafy gall disease. [1] R. fascians is the only phytopathogenic member of the genus Rhodococcus ; its host range includes both dicotyledonous and monocotyledonous hosts. Because it commonly afflicts tobacco ( Nicotiana ) plants, it is an agriculturally significant pathogen.

Contents

Physiology and morphology

R. fascians is an aerobic, pleiomorphic bacterium of the actinomycetes that is nonmotile and does not form spores. [2] When grown on the surface of an agar plate, colonies are orange in color and appear both smooth or rough.[ citation needed ]

Virulence

R. fascians can be a pathogen of plants, both angiosperm or gymnosperm. Infected plants show typical symptoms, such as leaf deformation, witches broom and leaf gall, which development depends on the plant's cultivar, plant's age, and the bacterial strain. [1]

Leaf deformation consists of widening of parenchyma and growth of vascular system, resulting in wrinkling of laminae and widening of veins. Leafy gall is a gall originated from a bud which would not develop under normal conditions. All effects coming from the infection of R. fascians do not depend on plant cells' transformation (as they do in Agrobacterium tumefaciens or Agrobacterium rhizogenes ), but on expression of virulence-related genes of bacterium and on the production of compounds that can interfere with normal plant growth and development. During the infection, R. fascians usually stays outside vegetal tissues, near a junction or cavity of a plant's cell walls, maybe to avoid environmental stresses. Presence of R. fascians was also observed in intercellular spaces inside tissues (in leaf or galls) and even inside cell walls. Presence of R. fascians on the infected plant is necessary, not only for the initiation of infection, but also for its maintenance.[ citation needed ]

Virulence genes

Virulence in R. fascians is controlled by genes on a plasmid (strains lacking that plasmid are not virulent) and on the chromosome. Using deletion mutations, it was possible to identify three loci on the plasmid: fas, att, and hyp, and one locus on the chromosome, vic. [2] [3] [4]

The fas is an operon made of six genes (orf 1-6) and a regulatory gene, fasR. Because deletions of some fas genes give a non-virulent phenotype, for fas a main role in virulence was proposed . [3] Gene fasR is an araC-like transcriptional regulator. Its transcription can be induced in vitro in cultures containing certain carbon sources (such as glucose, sucrose, arabinose, glycerol, pyruvate, mannitol, mannose) or nitrogen sources (such as histidine), and is influenced by culture pH and optical density. [3] Also, fasR can be induced by gall extract created by virulent strain. The operon codifies for genes involved in cytokinin synthesis and degradation (orf 4,5,6), in particular for an isopentenyl transferase, a cytokinin oxidase and a glutation-s transferase. The orf1,2,3 transcribe for a cytochrome 450, a ferridoxine containing also a pyruvate dehydrogenase alfa-like domain and a pyruvate dehydrogenase beta subunit. It was supposed that the first three genes supply energy for the synthesis and degradation of cytokinin, performed by the last three genes of the operon: R. fascians can actually produce and degrade zeatin and isopentenil adenine. [3] The compound cytokin oxidase (orf4) can also create adenine with a reactive nitrogen in position 6, which can react with other lateral chains, to form cytokininn-like compounds, more efficient in inducing plant tissue growth. [3]

The att is an operon composed of nine genes: attR, a transcriptional regulator, attX, a gene including domains for transmembrane localization (perhaps needed for exportation of compounds made by other att genes), and several genes attA-attH. [4] Many point and Δatt mutants show an attenuated virulence. [4]

Gene attR is a transcriptional factor including a helix-turn-helix motif. Its transcription is regulated by the same factors that regulate fasR transcription, but with a higher intensity, suggesting, with the attenuation of virulence in att mutants, that att may regulate fas transcription. Transcription of att operon is regulated with a quorum-sensing mechanism: indeed, density of cultures can influence transcription of attR, and leafy gall extracts coming from galls made by att mutant strains are less effective on transcription of attR. [4]

Genes attA-attH may be involved in synthesis of compounds needed for transcription of attR and attX. In fact, attA, attD & attH are involved in betalactamase synthesis, but no traces of those compounds were found in culture supernatants. [4]

The hyp codifies for an RNA-helicase; mutants for this gene are hypervirulent. Also, hyp is involved in post transcriptional control of virulence-related genes, maybe on fas products.

Operon vic is an operon made of five genes, located on the bacterial chromosome. [2] The only known gene is vicA, the fourth gene in the operon, whose product is a Mas homologue, a protein needed for the switch from citric acid cycle to glyoxylate cycle, both for metabolic reasons and to avoid glyoxylate accumulation, which is toxic for the bacteria. Mutations in vicA reduce virulence due to incapacity of R. fascians to resist glyoxylate accumulation. [2]

Induction of transcription in infected plant

In tobacco, infection of R. fascians leads to hyperexpression of a cytochrome P450, homologue to a gene involved in inactivation of abscisic acid in Arabidopsis thaliana , of a gibberellic acid oxidase, which inactivates this hormone and its precursors, a proline dehydrogenase, which has its transcription induced by cytokinin and turns proline into glutamic acid, and a factor involved in molybdenum cofactor, needed for sulfur, carbon and nitrogen metabolism control and for abscisic acid synthesis. [5]

Role of phytohormones during infection

All the effects of R. fascians infection can be attributed to hormone hyperdosage. In particular, most of the effects are connected to auxin and cytokinin, such as: formation of green islands on leaves, wrinkling of laminae, bud proliferation, delay of senescence, and inhibition of lateral roots. In fact, R. fascians can produce itself cytokinin, or cytokinin-like compounds: using orf4 and orf5 in the fas operon, it can stimulate infected plants to produce cytokinin, and it can produce indole-3-acetic acid itself, using a pathway starting from tryptophan and passing through production of 3-indol-piruvic acid and 3-indol-acetaldeid. [6] R.fascians can also degrade cytokinin to influence the cytokinin/auxin ratio.[ citation needed ]

Beside cytokinin and auxin, R. fascians acts on other hormones: in particular, it can block abscisic acid and gibberellic acid synthesis in infected plants. Abscisic acid represses growth, so a block of production is needed to allow proliferation of cells in leafy galls. Gibberellic acid controls cellular differentiation, so its block is needed for maintenance of meristematic cells and for their proliferation. [5]

Plant diseases

R. fascians causes diseases in several host plants including tobacco, small fruits (caneberries, strawberries) and ornamental plants (butterfly flowers, Primula, kalanchoes, Impatiens, geraniums, carnations)[ citation needed ]

Related Research Articles

<span class="mw-page-title-main">Plant hormone</span> Chemical compounds that regulate plant growth and development

Plant hormones are signal molecules, produced within plants, that occur in extremely low concentrations. Plant hormones control all aspects of plant growth and development, from embryogenesis, the regulation of organ size, pathogen defense, stress tolerance and through to reproductive development. Unlike in animals each plant cell is capable of producing hormones. Went and Thimann coined the term "phytohormone" and used it in the title of their 1937 book.

<span class="mw-page-title-main">Auxin</span> Plant hormone

Auxins are a class of plant hormones with some morphogen-like characteristics. Auxins play a cardinal role in coordination of many growth and behavioral processes in plant life cycles and are essential for plant body development. The Dutch biologist Frits Warmolt Went first described auxins and their role in plant growth in the 1920s. Kenneth V. Thimann became the first to isolate one of these phytohormones and to determine its chemical structure as indole-3-acetic acid (IAA). Went and Thimann co-authored a book on plant hormones, Phytohormones, in 1937.

<span class="mw-page-title-main">Cytokinin</span> Class of plant hormones promoting cell division

Cytokinins (CK) are a class of plant hormones that promote cell division, or cytokinesis, in plant roots and shoots. They are involved primarily in cell growth and differentiation, but also affect apical dominance, axillary bud growth, and leaf senescence.

Gibberellins (GAs) are plant hormones that regulate various developmental processes, including stem elongation, germination, dormancy, flowering, flower development, and leaf and fruit senescence. GAs are one of the longest-known classes of plant hormone. It is thought that the selective breeding of crop strains that were deficient in GA synthesis was one of the key drivers of the "green revolution" in the 1960s, a revolution that is credited to have saved over a billion lives worldwide.

<i>Agrobacterium tumefaciens</i> Bacterium, genetic engineering tool

Agrobacterium radiobacter is the causal agent of crown gall disease in over 140 species of eudicots. It is a rod-shaped, Gram-negative soil bacterium. Symptoms are caused by the insertion of a small segment of DNA, from a plasmid into the plant cell, which is incorporated at a semi-random location into the plant genome. Plant genomes can be engineered by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors.

<span class="mw-page-title-main">Jasmonate</span> Lipid-based plant hormones

Jasmonate (JA) and its derivatives are lipid-based plant hormones that regulate a wide range of processes in plants, ranging from growth and photosynthesis to reproductive development. In particular, JAs are critical for plant defense against herbivory and plant responses to poor environmental conditions and other kinds of abiotic and biotic challenges. Some JAs can also be released as volatile organic compounds (VOCs) to permit communication between plants in anticipation of mutual dangers.

<span class="mw-page-title-main">Abscisic acid</span> Plant hormone

Abscisic acid is a plant hormone. ABA functions in many plant developmental processes, including seed and bud dormancy, the control of organ size and stomatal closure. It is especially important for plants in the response to environmental stresses, including drought, soil salinity, cold tolerance, freezing tolerance, heat stress and heavy metal ion tolerance.

<span class="mw-page-title-main">Transfer DNA</span>

The transfer DNA is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as Agrobacterium tumefaciens and Agrobacterium rhizogenes . The T-DNA is transferred from bacterium into the host plant's nuclear DNA genome. The capability of this specialized tumor-inducing (Ti) plasmid is attributed to two essential regions required for DNA transfer to the host cell. The T-DNA is bordered by 25-base-pair repeats on each end. Transfer is initiated at the right border and terminated at the left border and requires the vir genes of the Ti plasmid.

<span class="mw-page-title-main">Callus (cell biology)</span> Growing mass of unorganized plant parenchyma cells

Plant callus is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells are those cells that cover a plant wound. In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro. The culture medium is supplemented with plant growth regulators, such as auxin, cytokinin, and gibberellin, to initiate callus formation or somatic embryogenesis. Callus initiation has been described for all major groups of land plants.

<span class="mw-page-title-main">Hydrotropism</span>

Hydrotropism is a plant's growth response in which the direction of growth is determined by a stimulus or gradient in water concentration. A common example is a plant root growing in humid air bending toward a higher relative humidity level.

<span class="mw-page-title-main">Ti plasmid</span>

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<span class="mw-page-title-main">Indole-3-acetic acid</span> Chemical compound

Indole-3-acetic acid is the most common naturally occurring plant hormone of the auxin class. It is the best known of the auxins, and has been the subject of extensive studies by plant physiologists. IAA is a derivative of indole, containing a carboxymethyl substituent. It is a colorless solid that is soluble in polar organic solvents.

<span class="mw-page-title-main">Fasciation</span> Condition of abnormal growth in vascular plants

Fasciation, also known as cresting, is a relatively rare condition of abnormal growth in vascular plants in which the apical meristem, which normally is concentrated around a single point and produces approximately cylindrical tissue, instead becomes elongated perpendicularly to the direction of growth, thus producing flattened, ribbon-like, crested, or elaborately contorted tissue. Fasciation may also cause plant parts to increase in weight and volume in some instances. The phenomenon may occur in the stem, root, fruit, or flower head.

<span class="mw-page-title-main">Abscission</span> Shedding of various parts of an organism

Abscission is the shedding of various parts of an organism, such as a plant dropping a leaf, fruit, flower, or seed. In zoology, abscission is the intentional shedding of a body part, such as the shedding of a claw, husk, or the autotomy of a tail to evade a predator. In mycology, it is the liberation of a fungal spore. In cell biology, abscission refers to the separation of two daughter cells at the completion of cytokinesis.

<span class="mw-page-title-main">Gibberellic acid</span> Chemical compound

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<i>Rhodococcus</i> Genus of bacteria

Rhodococcus is a genus of aerobic, nonsporulating, nonmotile Gram-positive bacteria closely related to Mycobacterium and Corynebacterium. While a few species are pathogenic, most are benign, and have been found to thrive in a broad range of environments, including soil, water, and eukaryotic cells. Some species have large genomes, including the 9.7 megabasepair genome of Rhodococcus sp. RHA1.

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<span class="mw-page-title-main">Somatic embryogenesis</span> Method to derive a plant or embryo from a single somatic cell

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References

  1. 1 2 Goethals K, Vereecke D, Jaziri M, Van Montagu M, Holsters M (2001). "Leafy gall formation by Rhodococcus fascians". Annu Rev Phytopathol. 39: 27–52. doi:10.1146/annurev.phyto.39.1.27. PMID   11701858.
  2. 1 2 3 4 Vereecke D, Cornelis K, Temmerman W, et al. (February 2002). "Chromosomal Locus That Affects Pathogenicity of Rhodococcus fascians". J. Bacteriol. 184 (4): 1112–20. doi:10.1128/jb.184.4.1112-1120.2002. hdl:1854/LU-322105. PMC   134788 . PMID   11807072.
  3. 1 2 3 4 5 Temmerman W, Vereecke D, Dreesen R, Van Montagu M, Holsters M, Goethals K (October 2000). "Leafy Gall Formation Is Controlled by fasR, an AraC-Type Regulatory Gene in Rhodococcus fascians". J. Bacteriol. 182 (20): 5832–40. doi:10.1128/JB.182.20.5832-5840.2000. PMC   94707 . PMID   11004184.
  4. 1 2 3 4 5 Maes T, Vereecke D, Ritsema T, et al. (October 2001). "The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound". Mol. Microbiol. 42 (1): 13–28. doi: 10.1046/j.1365-2958.2001.02615.x . PMID   11679063. S2CID   11681436.
  5. 1 2 Simon-Mateo C, Depuydt S, De Oliveira Manes CL, Cnudde F, Holsters M, Goethals K, Vereeke D (2006). "The phytopathogen Rhodococcus fascians breaks apical dominance and activate auxiliary meristems by inducing plant genes involved in hormones metabolism". Molecular Plant Pathology. 7 (2): 103–12. doi: 10.1111/j.1364-3703.2006.00322.x . PMID   20507431.
  6. Vandeputte O, Oden S, Mol A, et al. (March 2005). "Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues". Appl. Environ. Microbiol. 71 (3): 1169–77. Bibcode:2005ApEnM..71.1169V. doi:10.1128/AEM.71.3.1169-1177.2005. PMC   1065166 . PMID   15746315.