Carbonyl reductase (NADPH)

carbonyl reductase (NADPH)
carbonyl reductase tetramer, Mouse
Identifiers
EC no.	1.1.1.184
CAS no.	89700-36-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

Ribbon diagram of human carbonyl reductase 1, in complex with NADPH and a small molecule inhibitor (not shown). From PDB: 1WMA ​.

In enzymology, a carbonyl reductase (NADPH) (EC 1.1.1.184) is an enzyme that catalyzes the chemical reaction

R-CO-R' + NADPH + H⁺${\displaystyle \rightleftharpoons }$ :R-CHOH-R' + NADP⁺

Thus, the two products of this enzyme are R-CHOH-R' and NADP⁺, whereas its 3 substrates are R-CO-R', NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is secondary-alcohol:NADP⁺ oxidoreductase. Other names in common use include aldehyde reductase 1, prostaglandin 9-ketoreductase, xenobiotic ketone reductase, NADPH-dependent carbonyl reductase, ALR3, carbonyl reductase, nonspecific NADPH-dependent carbonyl reductase, aldehyde reductase 1, and carbonyl reductase (NADPH). This enzyme participates in arachidonic acid metabolism, and has recently been shown to catabolize S-Nitrosoglutathione, as a means to degrade NO in an NADPH-dependent manner.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1CYD, 1WMA, 2HRB, and 2PFG.

References

• Ahmed NK, Felsted RL, Bachur NR (1978). "Heterogeneity of anthracycline antibiotic carbonyl reductases in mammalian livers". Biochem. Pharmacol. 27 (23): 2713–9. doi:10.1016/0006-2952(78)90047-3. PMID   31888.
• Lin YM, Jarabak J (1978). "Isolation of two proteins with 9-ketoprostaglandin reductase and NADP-linked 15-hydroxyprostaglandin dehydrogenase activities and studies on their inhibition". Biochem. Biophys. Res. Commun. 81 (4): 1227–34. Bibcode:1978BBRC...81.1227L. doi:10.1016/0006-291X(78)91267-6. PMID   666816.
• Wermuth B (1981). "Purification and properties of an NADPH-dependent carbonyl reductase from human brain. Relationship to prostaglandin 9-ketoreductase and xenobiotic ketone reductase". J. Biol. Chem. 256 (3): 1206–13. doi: 10.1016/S0021-9258(19)69950-3 . PMID   7005231.