Cellular agriculture

Cellular agriculture focuses on the production of agricultural products from cell cultures using a combination of biotechnology, tissue engineering, molecular biology, and synthetic biology to create and design new methods of producing proteins, fats, and tissues that would otherwise come from traditional agriculture. ^[1] Most of the industry is focused on animal products such as meat, milk, and eggs, produced in cell culture rather than raising and slaughtering farmed livestock which is associated with substantial global problems of detrimental environmental impacts (e.g. of meat production), animal welfare, food security and human health. ^{[2] [3] [4] [5]} Cellular agriculture is a field of the biobased economy. The most well known cellular agriculture concept is cultured meat.

History

For a chronological guide, see Timeline of cellular agriculture.

Although cellular agriculture is a nascent scientific discipline, cellular agriculture products were first commercialized in the late 20th century with insulin and rennet. ^[6]

On March 24, 1990, the FDA approved a bacterium that had been genetically engineered to produce rennet, making it the first genetically engineered product for food. ^[7] Rennet is a mixture of enzymes that turns milk into curds and whey in cheese making. Traditionally, rennet is extracted from the inner lining of the fourth stomach of calves. Today, cheese making processes use rennet enzymes from genetically engineered bacteria, fungi, or yeasts because they are unadulterated, more consistent, and less expensive than animal-derived rennet. ^[8]

In 2004, Jason Matheny founded New Harvest, whose mission is to "accelerate breakthroughs in cellular agriculture". ^[9] New Harvest is the only organization focused exclusively on advancing the field of cellular agriculture and provided the first PhD funding specifically for cellular agriculture, at Tufts University. ^[10]

By 2014, IndieBio, a synthetic biology accelerator in San Francisco, has incubated several cellular agriculture startups, hosting Muufri (making milk from cell culture, now Perfect Day Foods), The EVERY Company (making egg whites from cell culture), Gelzen (making gelatin from bacteria and yeast, now Geltor), Afineur (making cultured coffee beans) and Pembient (making rhino horn). Muufri and The EVERY Company were both initially sponsored by New Harvest.

In 2015, Mercy for Animals created The Good Food Institute , which promotes plant-based and cellular agriculture. ^[11]

Also in 2015, Isha Datar coined the term "cellular agriculture" (often shortened to "cell ag") in a New Harvest Facebook group. ^{[12] [13]}

On July 13, 2016, New Harvest hosted the world's first international conference on cellular agriculture in San Francisco, California. ^[9] The day after the conference, New Harvest hosted the first closed-door workshop for industry, academic, and government stakeholders in cellular agriculture. ^[14]

Research tools

Several key research tools are at the foundation of research in cellular agriculture. These include: ^[15]

Cell lines

A fundamental missing piece in the advancement of cultured meat is the availability of the appropriate cellular materials. While some methods and protocols from human and mouse cell culture may apply to agricultural cellular materials, it has become clear that most do not. This is evidenced by the fact that established protocols for creating human and mouse embryonic stem cells have not succeeded in establishing ungulate embryonic stem cell lines. ^{[16] [17] [18]}

The ideal criteria for cell lines for the purpose of cultured meat production include immortality, high proliferative ability, surface independence, serum independence, and tissue-forming ability. The specific cell types most suitable for cellular agriculture are likely to differ from species to species. ^{[19] [20]}

Growth media

Conventional methods for growing animal tissue in culture involve the use of fetal bovine serum (FBS). FBS is a blood product extracted from fetal calves. This product supplies cells with nutrients and stimulating growth factors, but is unsustainable and resource-heavy to produce, with large batch-to-batch variation. ^[21] Cultured meat companies have been putting significant resources into alternative growth media.

After the creation of the cell lines, efforts to remove serum from the growth media are key to the advancement of cellular agriculture as fetal bovine serum has been the target of most criticisms of cellular agriculture and cultured meat production. It is likely that two different media formulations will be required for each cell type: a proliferation media, for growth, and a differentiation media, for maturation. ^[22]

Scaling technologies

As biotechnological processes are scaled, experiments start to become increasingly expensive, as bioreactors of increasing volume will have to be created. Each increase in size will require a re-optimization of various parameters such as unit operations, fluid dynamics, mass transfer, and reaction kinetics.

Scaffold materials

For cells to form tissue, it is helpful for a material scaffold to be added to provide structure. Scaffolds are crucial for cells to form tissues larger than 100 μm across. An ideal scaffold must be non-toxic for the cells, edible, and allow for the flow of nutrients and oxygen. It must also be cheap and easy to produce on a large scale without the need for animals.

3D tissue systems

The final phase for creating cultured meat involves bringing together all the previous pieces of research to create large (>100 μm in diameter) pieces of tissue that can be made of mass-produced cells without the need for serum, where the scaffold is suitable for cells and humans.

Applications

While the majority of the discussion has been around food applications, particular cultured meat, cellular agriculture can be used to create any kind of agricultural product, including those that never involved animals to begin with, like Ginkgo Biowork's fragrances.

Meat

Main article: Cultured meat

Cultured meat (also known by other names) is a meat produced by in vitro cell cultures of animal cells. ^[23] It is a form of cellular agriculture, with such agricultural methods being explored in the context of increased consumer demand for protein. ^[24]

Cultured meat is produced using tissue engineering techniques traditionally used in regenerative medicines. ^[25] The concept of cultured meat was introduced to wider audiences by Jason Matheny in the early 2000s after he co-authored a paper ^[26] on cultured meat production and created New Harvest, the world's first nonprofit organization dedicated to in-vitro meat research. ^[27]

Cultured meat may have the potential to address substantial global problems of the environmental impact of meat production, animal welfare, food security and human health. ^{[2] [3] [4] [28] [29] [30]} Specifically, it can be thought of in the context of the mitigation of climate change. ^[24]

The Meat Revolution, a lecture at the World Economic Forum by Mark Post of the University of Maastricht about in vitro meat

A video by New Harvest and Xprize explaining the development of cultured meat and a "post-animal bio-economy" driven by lab-grown protein (meat, eggs, milk)

In 2013, professor Mark Post at Maastricht University pioneered a proof-of-concept for cultured meat by creating the first hamburger patty grown directly from cells. Since then, other cultured meat prototypes have gained media attention: SuperMeat opened a farm-to-fork restaurant called "The Chicken" ^[31] in Tel Aviv to test consumer reaction to its "Chicken" burger, ^[32] while the "world's first commercial sale of cell-cultured meat" occurred in December 2020 at the Singapore restaurant "1880", where cultured meat manufactured by the US firm Eat Just was sold. ^[33]

While most efforts in the space focus on common meats such as pork, beef, and chicken which comprise the bulk of consumption in developed countries, ^[34] some new companies such as Orbillion Bio have focused on high end or unusual meats including Elk, Lamb, Bison, and the prized Wagyu strain of beef. ^[35] Avant Meats has brought cultured grouper fish to market ^[36] as other companies have started to pursue cultivating additional fish species and other seafood. ^[37]

The production process is constantly evolving, driven by multiple companies and research institutions. ^[38] The applications of cultured meat have led to ethical, health, environmental, cultural, and economic discussions. ^[39] In terms of market strength, data published by the non-governmental organization Good Food Institute found that in 2021 cultivated meat companies attracted $140 million in Europe alone. ^[24] Currently cultured meat is served at special events and few high end restaurants, mass production of cultured meat has not started yet.

In 2021, researchers presented a bioprinting method to produce steak-like cultured meat.

In 2020, the world's first regulatory approval for a cultivated meat product was awarded by the Government of Singapore. The chicken meat was grown in a bioreactor in a fluid of amino acids, sugar, and salt. ^[42] The chicken nuggets food products are ~70% lab-grown meat, while the remainder is made from mung bean proteins and other ingredients. The company pledged to strive for price parity with premium "restaurant" chicken servings. ^{[43] [44]}

For an overview of start-up companies and non-profit organisations working on cellular meat, see Cultured meat § Companies.

Dairy

• Perfect Day is a San Francisco-based startup that started as the New Harvest Dairy Project and was incubated by IndieBio in 2014. Perfect Day is making dairy from yeast instead of cows. ^{[45] [46]} The company changed its name from Muufri to Perfect Day in August 2016. ^[47]

• New Culture is a San Francisco-based startup that was incubated by IndieBio in 2019. ^[48] New Culture makes mozzarella cheese using casein protein (dairy protein) made by microbes instead of cows. ^{[49] [50] [51]}

• Real Vegan Cheese based in the San Francisco Bay-area is a grass-roots, non-profit Open Science collective working out of two open community labs and was spun out of the International Genetically Engineered Machine (iGEM) competition in 2014. ^[52] Real Vegan Cheese are making cheese using casein protein (dairy protein) made by microbes instead of cows. ^{[53] [54] [55] [56]}

• Formo, based in Germany, is a startup making dairy products using microbial precision fermentation. ^[57]
• Imagindairy, based in Israel, is a startup attempting to create milk proteins from bioengineered yeast. ^{[58] [59]} In 2024 it had received FDA and Israeli Ministry of Health approval for its products. ^[60]
• Remilk, based in Israel, is a startup attempting to create milk proteins from bioengineered yeast. In 2022 it had received FDA approval for its products. ^[61]
• Wilk, based in Israel, is a startup attempting to produce human mother milk ingredients using cells from breast reduction surgeries, to supplement infant formulas. ^[62]
• NewMoo, based in Israel, is a startup attempting to create casein protein within the seeds of genetically modified plants. ^[63]
• Real Deal Milk, based in Spain, is a startup attempting to create milk proteins from bioengineered microbes. ^[64]
• Opalia, based in Canada, is a startup attempting to produce milk from cows' mammary cells. ^[65]
• De Novo Dairy, based in South-Africa, is a startup attempting to produce human mother milk ingredients using cells from breast reduction surgeries, to supplement infant formulas. ^[66]
• Cultivated Biosciences, based in Switzerland, is a startup attempting to produce fats from non-GMO yeast to make plant based milk more creamy. ^[67]
• Naturopy, based in France, is a startup attempting to create milk proteins from bioengineered yeast. ^[68]

Eggs

• The EVERY Company is a San Francisco-based startup that started as the New Harvest Egg Project and was incubated by IndieBio in 2015. The EVERY Company is making egg whites from yeast instead of eggs. ^[69]

Gelatin

• Geltor is a San Francisco-based startup that was incubated by IndieBio in 2015. Geltor is developing a proprietary protein production platform that uses bacteria and yeast to produce gelatin. ^{[70] [71]}

Coffee

In 2021, media outlets reported that the world's first synthetic coffee products have been created by two biotechnology companies, still awaiting regulatory approvals for near-term commercialization. ^{[72] [73] [74] [75]} Such products – which can be produced via cellular agriculture in bioreactors ^[74] and for which multiple companies' R&D have acquired substantial funding – may have equal or highly similar effects, composition and taste as natural products but use less water, generate less carbon emissions, require less labor ^{[73] [ additional citation(s) needed ]} and cause no deforestation. ^[72] Cell-cultured coffee is a much more radical approach to the multiple challenges that traditional coffee is facing. While 100% coffee, cell-cultured coffee is cultivated in the lab from coffee cells to deliver, after drying, a powder that can be roasted and extracted. ^[75]

Horseshoe crab blood

• Sothic Bioscience is a Cork-based startup incubated by IndieBio in 2015. Sothic Bioscience is building a platform for biosynthetic horseshoe crab blood production. Horseshoe crab blood contains limulus amebocyte lysate (LAL), which is the gold standard in validating medical equipment and medication. ^{[76] [77]}

Fish

Cellular agriculture could be used for commercial fish feed.

• Finless Foods is working to develop and mass manufacture marine animal food products. ^[78]
• Wild Type is a San Francisco-based startup focused on creating cultured meat to address items such as climate change, food security, and health. ^{[79] [80]}

Fragrances

• Ginkgo Bioworks is a Boston-based organism design company culturing fragrances and designing custom microbes. ^[81]

Silk

See also: § Similar fields of research and production

• Spiber is a Japan-based company decoding the gene responsible for the production of fibroin in spiders and then bioengineering bacteria with recombinant DNA to produce the protein, which they then spin into their artificial silk. ^{[82] [83]}
• Bolt Threads is a California-based company creating engineered silk fibers based on proteins found in spider silk that can be produced at commercial scale. Bolt examines the DNA of spiders and then replicates those genetic sequences in other ingredients to create a similar silk fiber. Bolt's silk is made primarily of sugar, water, salts, and yeast. Through a process called wet spinning, this liquid is spun into fiber, similar to the way fibers like acrylic and rayon are made. ^{[84] [85] [86]}

Leather

• Modern Meadow is a Brooklyn-based startup growing collagen, a protein found in animal skin, to make biofabricated leather. ^[87]

Pet food

• Clean Meat cluster lists Because Animals, ^[88] Wild Earth and Bond Pet Foods ^[89] as participants in developing pet foods that use cultured meat. ^[90]

Wood

In 2022, scientists reported the first 3D-printed lab-grown wood. It is unclear if it could ever be used on a commercial scale (e.g. with sufficient production efficiency and quality). ^{[91] [92]}

Issues

See also: Synthetic biology § Ethics

Degrowth, green growth and circular economy

Further information: Degrowth

The bioeconomy has largely been associated with visions of "green growth". ^[93] A study found that a "circular bioeconomy" may be "necessary to build a carbon neutral future in line with the climate objectives of the Paris Agreement". ^[94] However, some are concerned that with a focus or reliance on technological progress a fundamentally unsustainable socioeconomic model might be maintained rather than be changed. ^[95] Some are concerned it that may not lead to a ecologization of the economy but to an economization of the biological, "the living" and caution that potentials of non-bio-based techniques to achieve greater sustainability need to be considered. ^[95] A study found that the, as of 2019, current EU interpretation of the bioeconomy is "diametrically opposite to the original narrative of Baranoff and Georgescu-Roegen that told us that expanding the share of activities based on renewable resources in the economy would slow down economic growth and set strict limits on the overall expansion of the economy". ^[96] Furthermore, some caution that "Silicon Valley and food corporations" could use bioeconomy technologies for greenwashing and monopoly-concentrations. ^[97] The bioeconomy, its potentials, disruptive new modes of production and innovations may distract from the need for systemic structural socioeconomic changes ^{[98] [99]} and provide a false illusion of technocapitalist utopianism/optimism that suggests technological fixes ^[100] may make it possible to sustain contemporary patterns and structures, pre-empting structural changes.

Unemployment and work reallocation

Further information: Technological unemployment

Many farmers depend on conventional methods of producing crops and many of them live in developing economies. ^[101] Cellular agriculture for products such as synthetic coffee could, if the contemporary socioeconomic context (the socioeconomic system's mechanisms such as incentives and resource distribution mechanisms like markets) remains unaltered (e.g. in nature, purposes, scopes, limits and degrees), threaten their employment and livelihoods as well as the respective nation's economy and social stability. A study concluded that "given the expertise required and the high investment costs of the innovation, it seems unlikely that cultured meat immediately benefits the poor in developing countries" and emphasized that animal agriculture is often essential for the subsistence for farmers in poor countries. ^[102] However, not only developing countries may be affected. ^[103]

Patents, intellectual property and monopolies

Observers worry that the bioeconomy will become as opaque and free of accountability as the industry it attempts to replace, that is the current food system. The fear is that its core products will be mass-produced, nutritionally dubious meat sold at the homogeneous fast-food joints of the future. ^[97]

The medical community has warned that gene patents can inhibit the practice of medicine and progress of science. ^[104] This can also apply to other areas where patents and private intellectual property licenses are being used, often entirely preventing the use and continued development of knowledge and techniques for many years or decades. On the other hand, some worry that without intellectual property protection as the type of R&D-incentive, particularly to current degrees and extents, companies would no longer have the resources or motives/incentives to perform competitive, viable biotech research – as otherwise they may not be able to generate sufficient returns from initial R&D investment or less returns than from other expenditures that are possible. ^[105] "Biopiracy" refers to "the use of intellectual property systems to legitimize the exclusive ownership and control over biological resources and biological products that have been used over centuries in non-industrialized cultures". ^[106]

Rather than leading to sustainable, healthy, inexpensive, safe, accessible food being produced with little labor locally – after knowledge- and technology transfer and timely, efficient innovation – the bioeconomy may lead to aggressive monopoly-formation and exacerbated inequality. ^{[107] [108] [97] [ additional citation(s) needed ]} For instance, while production costs may be minimal, costs – including of medicine ^[109] – may be high.

Innovation management, public spending and governance

See also: Strategic planning

It has been argued that public investment would be a tool governments should use to regulate and license cellular agriculture. Private firms and venture capital would likely seek to maximise investor value rather than social welfare. ^[97] Moreover, radical innovation is considered to be more risky, "and likely involves more information asymmetry, so that private financial markets may imperfectly manage these frictions". Governments may also help to coordinate "since several innovators may be needed to push the knowledge frontier and make the market profitable, but no single company wants to make the early necessary investments". And investments in the relevant sectors seem to be a bottleneck hindering the transition toward a bioeconomy. ^[110] Governments could also help innovators that lack the network "to naturally obtain the visibility and political influence necessary to obtain public funds" and could help determine relevant laws. ^[111] By establishing supporting infrastructure for entrepreneurial ecosystems they can help creating a beneficial environment for innovative bioeconomy startups. ^[112] Enabling such bioeconomy startups to act on the opportunities provided through the bioeconomy transformation further contributes to its success. ^[113]

Academic programs

New Harvest Cultured Tissue Fellowship at Tufts University

A joint program between New Harvest and the Tissue Engineering Research Center (TERC), an NIH-supported initiative established in 2004 to advance tissue engineering. The fellowship program offers funding for Masters and PhD students at Tufts university who are interested in bioengineering tunable structures, mechanics, and biology into 3D tissue systems related to their utility as foods. ^[114]

Conferences

New Harvest Conference

New Harvest brings together pioneers in the cellular agriculture and new, interested parties from industry and academia to share relevant learnings for cellular agriculture's path moving forward. The Conference has been held in San Francisco, California, Brooklyn, New York, and is currently held in Cambridge, Massachusetts. ^[115]

Industrializing Cell-Based Meats & Seafood Summit

The 3rd Annual Industrializing Cell-Based Meats & Seafood Summit is the only industry-led forum uniting key decision-makers from biotech and food tech, leading food and meat companies, and investors to discuss key operational and technical challenges for the development of cell-based meats and seafood. ^[116]

International Scientific Conference on Cultured Meat

The International Scientific Conference on Cultured Meat began in collaboration with Maastricht University in 2015, and brings together an international group of scientists and industry experts to present the latest research and developments in cultured meat. It takes place annually in Maastricht, The Netherlands. ^[117]

Good Food Conference

The GFI conference is an event focused on accelerating the commercialization of plant-based and clean meat. ^[118]

Cultured Meat Symposium

The Cultured Meat Symposium is a conference held in Silicon Valley highlighting top industry insights of the clean meat revolution. ^{[119] [120]}

Alternative Protein Show

The Alternative Protein Show is a "networking event" to facilitate collaboration in the "New Protein Landscape", which includes plant-based and cellular agriculture. ^[121]

New Food Conference

The New Food Conference is an industry-oriented event that aims to accelerate and empower innovative alternatives to animal products by bringing together key stakeholders. It is Europe's first and biggest conference on new-protein solutions. ^[122]

In the media

Books

• Clean Meat: How Growing Meat Without Animals Will Revolutionize Dinner and the World is a book about cellular agriculture written by animal activist Paul Shapiro (author). The book reviews startup companies that are currently working towards mass-producing cellular agriculture products. ^{[123] [124] [125]}
• Meat Planet: Artificial Flesh and the Future of Food by Benjamin Aldes Wurgaft is the result of five years researching cellular agriculture, and explores the quest to generate meat in the lab, asking what it means to imagine that this is the future of food. It is published by the University of California Press. ^[126]
• Where do hot dogs come from? A Children's Book about Cellular Agriculture by Anita Broellochs, Alex Shirazi and Illustrated by Gabriel Gonzalez turns a family BBQ into a scientific story explaining how hot dogs are made with cellular agriculture technologies. The book was launched on Kickstarter on July 20, 2021. ^{[127] [128]}

Podcasts

• Cultured Meat and Future Food is a podcast about clean meat and future food technologies hosted by Alex Shirazi, ^[129] a mobile User Experience Designer based in Menlo Park, California, whose current projects focus on retail technology. The podcast features interviews with industry professionals from startups, investors, and non-profits working on cellular agriculture. ^{[130] [131]}

Similar fields of research and production

See also: Timeline of biotechnology

• Microbial food cultures and genetically engineered microbial production (e.g. of spider silk ^{[132] [133]} or solar-energy-based protein powder) ^{[134] [135]}
• Controlled self-assembly of plant proteins (e.g. of spider silk similar plant-proteins-based plastics alternatives) ^{[136] [137]}
• Cell-free artificial synthesis (see Biobased economy#Agriculture)
• Imitation foods (e.g. meat analogues and milk substitutes)

References

1. "A Closer Look at Cellular Agriculture and the Processes Defining It - AgFunderNews". 2016-07-05. Retrieved 2016-08-05.
2. Bryant, Christopher J (3 August 2020). "Culture, meat, and cultured meat". Journal of Animal Science. 98 (8): skaa172. doi:10.1093/jas/skaa172. ISSN   0021-8812. PMC   7398566 . PMID   32745186.
3. Hong, Tae Kyung; Shin, Dong-Min; Choi, Joonhyuk; Do, Jeong Tae; Han, Sung Gu (May 2021). "Current Issues and Technical Advances in Cultured Meat Production: AReview". Food Science of Animal Resources. 41 (3): 355–372. doi:10.5851/kosfa.2021.e14. ISSN   2636-0772. PMC   8112310 . PMID   34017947.
4. Treich, Nicolas (1 May 2021). "Cultured Meat: Promises and Challenges". Environmental and Resource Economics. 79 (1): 33–61. doi:10.1007/s10640-021-00551-3. ISSN   1573-1502. PMC   7977488 . PMID   33758465.
5. Mattick, CS (January 2018). "Cellular agriculture: The coming revolution in food production". Bulletin of the Atomic Scientists. 74 (1): 32–35. Bibcode:2018BuAtS..74a..32M. doi:10.1080/00963402.2017.1413059. S2CID   149404346.
6. "About" . Retrieved 2016-08-08.
7. "FDA approves 1st genetically engineered product for food". 1990-03-24.
8. "Case Studies: Chymosin". Archived from the original on 2016-05-22.
9. Street, 1401 21st; Sacramento, Suite 4556; Usa, Ca 95811-5226. "Who We Are".
10. "Cellular Agriculture at Tufts University". Archived from the original on 2016-08-07.
11. Bowie, Richard. "MFA Launches New Sister Organization". VegNews.com.
12. Crosser, Nate (13 April 2021). "Cellular agriculture landscape". Fifth Industrial.
13. "Useful Resources". Cellular Agriculture Australia.
14. Harvest, New (2016-08-04). "Notes from the 2016 Cellular Agriculture Innovators' Workshop". Medium. Retrieved 2016-08-05.
15. Talbot, Neil C.; Blomberg, Le Ann (2008-01-01). "The Pursuit of ES Cell Lines of Domesticated Ungulates". Stem Cell Reviews. 4 (3): 235–254. doi:10.1007/s12015-008-9026-0. PMID   18612851. S2CID   1490897.
16. Keefer, CL; Pant, D; Blomberg, L; Talbot, NC (2007). "Challenges and prospects for the establishment of embryonic stem cells of domesticated ungulates". Animal Reproduction Science. 98 (1–2): 147–68. doi:10.1016/j.anireprosci.2006.10.009. PMID   17097839.
17. Talbot, NC; Le Ann, Blomberg (2008). "The pursuit of ES cell lines of domesticated ungulates". Stem Cell Rev. 4 (3): 235–154. doi:10.1007/s12015-008-9026-0. PMID   18612851. S2CID   1490897.
18. Nowak-Imialek, Monika; Niemann, Heiner (2016). "Embryonic Stem Cells and Fetal Development Models". Fetal Stem Cells in Regenerative Medicine. Stem Cell Biology and Regenerative Medicine. pp. 81–99. doi:10.1007/978-1-4939-3483-6_5. ISBN   978-1-4939-3481-2.
19. Cao, S; Wang, F; Liu, L (2013). "Isolation and Culture of Bovine Embryonic Stem Cells". Epiblast Stem Cells. Methods in Molecular Biology. Vol. 1074. pp. 111–23. doi:10.1007/978-1-62703-628-3_9. ISBN   978-1-62703-627-6. PMID   23975809.
20. Gandolfi, F; Pennarossa, G; Maffei, S; Brevini, T (2012). "Why is it so difficult to derive pluripotent stem cells in domestic ungulates?". Reprod Domest Anim. 47 (Suppl 5): 11–7. doi: 10.1111/j.1439-0531.2012.02106.x . PMID   22913556.
21. Van der Valk, J (2010). "Optimization of chemically defined cell culture media--replacing fetal bovine serum in mammalian in vitro methods". Toxicol in Vitro. 24 (4): 1053–63. doi:10.1016/j.tiv.2010.03.016. hdl: 1874/191398 . PMID   20362047. S2CID   205410680.
22. Agapakis, Christina (2012). "Steak of the Art: The Fatal Flaws of In Vitro Meat". Discover Magazine.
23. Datar, I (January 2010). "Possibilities for an in vitro meat production system". Innovative Food Science & Emerging Technologies. 11 (1): 13–22. doi:10.1016/j.ifset.2009.10.007.
24. De Lorenzo, Daniela (17 March 2022). "Dutch Parliament Approves Cultured Meat Tasting In The Netherlands". Forbes.com . Retrieved 8 April 2022.
25. Post, Mark (4 December 2013). "Medical technology to Produce Food". Journal of the Science of Food and Agriculture. 94 (6): 1039–1041. doi:10.1002/jsfa.6474. PMID   24214798.
26. Edelman, PD (3 May 2005). "Commentary: In Vitro-Cultured Meat Productionsystem". Tissue Engineering. 11 (5–6): 659–662. CiteSeerX   10.1.1.179.588 . doi:10.1089/ten.2005.11.659. PMID   15998207 . Retrieved 8 April 2018.
27. Schonwald, Josh (May 2009). "Future Fillet". The University of Chicago Magazine.
28. Bryant, Christopher J (1 August 2020). "Culture, meat, and cultured meat". Journal of Animal Science. 98 (8): skaa172. doi:10.1093/jas/skaa172. PMC   7398566 . PMID   32745186.
29. Treich, Nicolas (May 2021). "Cultured Meat: Promises and Challenges". Environmental and Resource Economics. 79 (1): 33–61. doi:10.1007/s10640-021-00551-3. PMC   7977488 . PMID   33758465.
30. Wood, Paul; Thorrez, Lieven; Hocquette, Jean-François; Troy, Declan; Gagaoua, Mohammed (2023-04-01). ""Cellular agriculture": current gaps between facts and claims regarding "cell-based meat"". Animal Frontiers. 13 (2): 68–74. doi: 10.1093/af/vfac092 .
31. Kolyohin, Nick (2 July 2021). "Feature: Israeli cultured meat company aims to redefine industry". Xinhua News Agency. Retrieved 2 July 2021.
32. Peters, Adele (5 November 2020). "At the first lab-grown meat restaurant, you can eat a 'cultured chicken' sandwich". Fast Company. Retrieved 18 January 2021.
33. Scully, Matthew (17 January 2021). "Hello Cultured Meat, Goodbye to the Cruelty of Industrial Animal Farming". National Review. Retrieved 18 January 2021.
34. "What is the most consumed meat in the world?" . Retrieved 14 October 2021.
35. "Investors eat up Orbillion Bio's plans for lab-grown Wagyu beef, elk and bison". 26 April 2021. Retrieved 14 October 2021.
36. "Lab-grown fish makes a debut in Hong Kong". 29 January 2021. Retrieved 14 October 2021.
37. "Seafood Without The Sea: Will Lab-Grown Fish Hook Consumers?". 5 May 2019. Retrieved 14 October 2021.
38. "Future Food - In Vitro Meat". futurefood.org. November 2018. Retrieved 26 November 2018.
39. Rohrheim, A (June 2016). "Cultured Meat". Sentience Politics. Archived from the original on 1 December 2018. Retrieved 26 November 2018.
40. "Japanese scientists produce first 3D-bioprinted, marbled Wagyu beef". New Atlas. 25 August 2021. Retrieved 21 September 2021.
41. Kang, Dong-Hee; Louis, Fiona; Liu, Hao; Shimoda, Hiroshi; Nishiyama, Yasutaka; Nozawa, Hajime; Kakitani, Makoto; Takagi, Daisuke; Kasa, Daijiro; Nagamori, Eiji; Irie, Shinji; Kitano, Shiro; Matsusaki, Michiya (24 August 2021). "Engineered whole cut meat-like tissue by the assembly of cell fibers using tendon-gel integrated bioprinting". Nature Communications. 12 (1): 5059. Bibcode:2021NatCo..12.5059K. doi:10.1038/s41467-021-25236-9. ISSN   2041-1723. PMC   8385070 . PMID   34429413.
42. Shanker, Deena (October 22, 2019). "These $50 Chicken Nuggets Were Grown in a Lab". Bloomberg.com. Archived from the original on February 25, 2020. Retrieved February 27, 2020.
43. Corbyn, Zoë (January 19, 2020). "Out of the lab and into your frying pan: the advance of cultured meat". the Guardian. Archived from the original on February 11, 2020. Retrieved February 27, 2020.
44. Ives, Mike (2 December 2020). "Singapore Approves a Lab-Grown Meat Product, a Global First". The New York Times. Archived from the original on 22 January 2021. Retrieved 16 January 2021.
45. "Muufri Milk". Archived from the original on 2016-06-09.
46. "Perfect Day: All the dairy you love, with none of the dairy cows". Perfect Day.
47. "Non-Animal Protein FAQs". Perfect Day.
48. "BRIEF: Kraft Heinz's VC invests in New Culture $3.5m seed round for cell-grown cheese". AgFunderNews. 2019-09-10. Retrieved 2019-09-16.
49. "Interview: Matt Gibson, CEO of New Culture Foods". www.cell.ag. Archived from the original on 2019-12-22. Retrieved 2019-09-16.
50. Sheikh, Knvul (2019-08-02). "Got Impossible Milk? The Quest for Lab-Made Dairy". The New York Times. ISSN   0362-4331 . Retrieved 2019-09-16.
51. "New Culture". www.newculturefood.com. Retrieved 2021-08-15.
52. "Real Vegan Cheese |" . Retrieved 2019-09-16.
53. Wohlsen, Marcus (2015-04-15). "Cow Milk Without the Cow Is Coming to Change Food Forever". Wired. ISSN   1059-1028 . Retrieved 2019-09-16.
54. "Real Vegan Cheese!". Indiegogo. Retrieved 2019-09-16.
55. Murray-Ragg, Nadia (2017-10-01). "Scientists Develop 'Real Vegan Cheese' Made From Lab 'Milk' | News". LIVEKINDLY. Retrieved 2019-09-16.
56. "Real Vegan Cheese". Real Vegan Cheese. Retrieved 2021-08-15.
57. "Formo - The Future Dairy from Berlin". formo.bio. Retrieved 2021-08-15.
58. "Imagindairy plans to cut out the cow and make milk from yeast". New Atlas. 2021-01-08. Retrieved 2021-08-15.
59. "Blood, brains and burgers: The future is lab-grown everything". New Atlas. 2021-08-11. Retrieved 2021-08-15.
60. Spiro, James (2024-11-26). "Imagindairy's animal-free dairy gets green light in Israel". CTech. Retrieved 2024-11-26.
61. Ashkenazi, Shani (2022-08-06). "Israeli cultivated dairy co Remilk wins FDA approval". Globes. Retrieved 2024-11-26.
62. Wrobel, Sharon (2023-04-04). "French dairy giant Danone leads $3.5m investment into Israeli cultured milk startup". Times of Israel.
63. Southey, Flora (2024-05-28). "Multiple milk proteins grown in a single plant? NewMoo makes liquid casein for animal-free cheese". FoodNavigator . Retrieved 2024-11-26.
64. Southey, Flora (2022-05-04). "'Will there still be dairy cows by 2050? We don't think so': Real Deal Milk taps microbes to make vegan cheese in Barcelona". FoodNavigator . Retrieved 2024-11-27.
65. "Cell-based dairy company Opalia brings animal-free milk closer to shelves - Food In CanadaFood In Canada". www.foodincanada.com. 2022-03-09. Retrieved 2024-11-27.
66. Southey, Flora (2022-03-14). "De Novo Dairy: Meet Africa's first precision fermentation player making human milk proteins for infant nutrition". FoodNavigator. Retrieved 2024-11-27.
67. Southey, Flora (2021-09-27). "Fermenting yeast into 'cream': Start-up adds richness and texture to plant-based dairy". FoodNavigator.com. Retrieved 2024-11-27.
68. Morrison, Oliver (2022-09-12). "Start-up harnesses caseins and dairy fatty acids to develop 'next generation' of cheese". FoodNavigator. Retrieved 2024-11-27.
69. Street, 1401 21st; Sacramento, Suite 4556; Usa, Ca 95811-5226. "Under Development".
70. "Gelzen Inc. – Making sustainable, animal-free gelatin". December 2, 2015. Archived from the original on August 19, 2016.
71. "Geltor". gelzen.com.
72. Lavars, Nick (20 September 2021). "Lab-grown coffee cuts out the beans and deforestation". New Atlas. Retrieved 18 October 2021.
73. Nittle, Nadra (16 October 2021). "Eco-friendly, lab-grown coffee is on the way, but it comes with a catch". The Guardian. Retrieved 26 October 2021.
74. "Sustainable coffee grown in Finland". VTT News. 15 September 2021. Retrieved 18 October 2021.
75. Jaloliddin Khushvakov, Sebastian E. W. Opitz, Nadja Plüss, Jasmin Sun, Linda Josefine Manthey, Heiko Rischer, and Chahan Yeretzian (2024). "Analytical Platform to Determine Similarities and Dissimilarities between Cell-Cultured Coffee and Farm-Grown Coffee". Journal of Food Science & Technology. doi: 10.1021/acsfoodscitech.4c00238 .
76. "Sothic Bioscience: Protecting human lives while preserving an ancient species". Archived from the original on 2016-07-29. Retrieved 2016-08-08.
77. "Lampstack". Archived from the original on 2018-03-01.
78. "Finless Foods – Finless Foods". Archived from the original on 2018-09-24. Retrieved 2018-11-22.
79. "Wild Type raises $3.5M to reinvent meat for the 21st century". 29 March 2018.
80. "Home". Wild Type. Archived from the original on 2019-08-27. Retrieved 2018-05-09.
81. "The Organism Company - Ginkgo Bioworks". Ginkgo Bioworks.
82. "Artificial "Spiber" silk is tougher than Kevlar". 12 July 2013.
83. "Spiber株式会社". Spiber株式会社.
84. "Bolt Threads". boltthreads.com.
85. Rao, Leena (May 11, 2016). "Bolt Threads Will Bring Its Spider Silk Fabric to Patagonia". Fortune.
86. "Bolt Threads". boltthreads.com. Retrieved 2021-08-15.
87. "Modern Meadow – Leather re-imagined". modernmeadow.com.
88. "Because Animals". Because Animals. Retrieved 2021-08-15.
89. "Bond Pet Foods - Animal-free & Protein-Rich Pet Food". Bond Pet Foods. Retrieved 2021-08-15.
90. "Wild Earth Announces the World's First Cell-Based Meat Developed for Dogs". www.businesswire.com. 26 October 2022.
91. Brahambhatt, Rupendra. "Science Scientists can now grow wood in a lab without cutting a single tree". Interesting Engineering. Retrieved 23 June 2022.
92. Beckwith, Ashley L.; Borenstein, Jeffrey T.; Velásquez-García, Luis F. (1 April 2022). "Physical, mechanical, and microstructural characterization of novel, 3D-printed, tunable, lab-grown plant materials generated from Zinnia elegans cell cultures". Materials Today. 54: 27–41. doi: 10.1016/j.mattod.2022.02.012 . ISSN   1369-7021. S2CID   247300299.
93. Hausknost, Daniel; Schriefl, Ernst; Lauk, Christian; Kalt, Gerald (April 2017). "A Transition to Which Bioeconomy? An Exploration of Diverging Techno-Political Choices". Sustainability. 9 (4): 669. doi: 10.3390/su9040669 .
94. Hoehn, Daniel; Laso, Jara; Margallo, María; Ruiz-Salmón, Israel; Amo-Setién, Francisco José; Abajas-Bustillo, Rebeca; Sarabia, Carmen; Quiñones, Ainoa; Vázquez-Rowe, Ian; Bala, Alba; Batlle-Bayer, Laura; Fullana-i-Palmer, Pere; Aldaco, Rubén (January 2021). "Introducing a Degrowth Approach to the Circular Economy Policies of Food Production, and Food Loss and Waste Management: Towards a Circular Bioeconomy". Sustainability. 13 (6): 3379. doi: 10.3390/su13063379 . hdl: 10902/21665 .
95. Pietzsch, Joachim (6 March 2020). Bioeconomy for Beginners. Springer Nature. ISBN   978-3-662-60390-1.
96. Giampietro, Mario (1 August 2019). "On the Circular Bioeconomy and Decoupling: Implications for Sustainable Growth". Ecological Economics. 162: 143–156. Bibcode:2019EcoEc.162..143G. doi: 10.1016/j.ecolecon.2019.05.001 . ISSN   0921-8009. S2CID   201329805.
97. "Man v food: is lab-grown meat really going to solve our nasty agriculture problem?". The Guardian. 29 July 2021. Retrieved 26 October 2021.
98. Forster, Piers M.; Forster, Harriet I.; Evans, Mat J.; Gidden, Matthew J.; Jones, Chris D.; Keller, Christoph A.; Lamboll, Robin D.; Quéré, Corinne Le; Rogelj, Joeri; Rosen, Deborah; Schleussner, Carl-Friedrich; Richardson, Thomas B.; Smith, Christopher J.; Turnock, Steven T. (7 August 2020). "Current and future global climate impacts resulting from COVID-19". Nature Climate Change. 10 (10): 913–919. Bibcode:2020NatCC..10..913F. doi: 10.1038/s41558-020-0883-0 . ISSN   1758-6798. S2CID   221019148.
99. Ripple, William J.; et al. (July 28, 2021), "World Scientists' Warning of a Climate Emergency 2021", BioScience, 71 (9): 894–898, doi:10.1093/biosci/biab079, hdl: 1808/30278 , retrieved July 29, 2021
100. McCormick, Kes; Kautto, Niina (2013). "The Bioeconomy in Europe: An Overview". Sustainability. 5 (6): 2589–2608. doi: 10.3390/su5062589 .
101. "Eco-friendly, lab-grown coffee is on the way, but it comes with a catch". The Guardian. 16 October 2021. Retrieved 26 October 2021.
102. Treich, Nicolas (2021). "Cultured Meat: Promises and Challenges". Environmental & Resource Economics. 79 (1): 33–61. Bibcode:2021EnREc..79...33T. doi:10.1007/s10640-021-00551-3. PMC   7977488 . PMID   33758465.
103. Newton, Peter; Blaustein-Rejto, Daniel (2021). "Social and Economic Opportunities and Challenges of Plant-Based and Cultured Meat for Rural Producers in the US". Frontiers in Sustainable Food Systems. 5: 10. doi: 10.3389/fsufs.2021.624270 . ISSN   2571-581X.
104. Andrews, LB (2000). "Genes and Patent Policy: Rethinking IP Rights". Nature Reviews Genetics. 3 (10): 803–8. doi:10.1038/nrg909. PMID   12360238. S2CID   13822192.
105. Marchant GE. 2007. Genomics, Ethics, and Intellectual Property. Intellectual Property Management in Health and Agricultural Innovation: A Handbook of Best Practices. Ch 1.5:29-38
106. Hamilton, Chris (15 December 2008). "Intellectual property rights, the bioeconomy and the challenge of biopiracy". Genomics, Society and Policy. 4 (3): 26. doi: 10.1186/1746-5354-4-3-26 . ISSN   1746-5354. PMC   5424966 . S2CID   35186396.
107. Braun, Veit (2021). "Tools of Extraction or Means of Speculation? Making Sense of Patents in the Bioeconomy". Bioeconomy and Global Inequalities. Springer International Publishing. pp. 65–84. doi: 10.1007/978-3-030-68944-5_4 . ISBN   978-3-030-68943-8. S2CID   236731518.{{cite book}}: |journal= ignored (help)
108. Birch, Kean (1 May 2017). "Rethinking Value in the Bio-economy: Finance, Assetization, and the Management of Value". Science, Technology, & Human Values. 42 (3): 460–490. doi:10.1177/0162243916661633. ISSN   0162-2439. PMC   5390941 . PMID   28458406. S2CID   1702910.
109. Löfgren, Hans (2009). "The Competition State and the Private Control of Healthcare". Global Health Governance. Palgrave Macmillan UK: 245–264. doi:10.1057/9780230249486_12. ISBN   978-1-349-30228-4.
110. Hinderer, Sebastian; Brändle, Leif; Kuckertz, Andreas (2021). "Transition to a Sustainable Bioeconomy". Sustainability. 13 (15): 8232. doi: 10.3390/SU13158232 .
111. Treich, Nicolas (1 May 2021). "Cultured Meat: Promises and Challenges". Environmental and Resource Economics. 79 (1): 33–61. Bibcode:2021EnREc..79...33T. doi:10.1007/s10640-021-00551-3. ISSN   1573-1502. PMC   7977488 . PMID   33758465.
112. Kuckertz, Andreas; Berger, Elisabeth S.C.; Brändle, Leif (2020). "Entrepreneurship and the sustainable bioeconomy transformation". Environmental Innovation and Societal Transitions. 37: 332–344. Bibcode:2020EIST...37..332K. doi: 10.1016/j.eist.2020.10.003 .
113. Hinderer, Sebastian; Kuckertz, Andreas (2022). "The bioeconomy transformation as an external enabler of sustainable entrepreneurship". Business Strategy and the Environment. 31 (7): 2947–2963. doi:10.1002/BSE.3056. hdl: 10419/266672 .
114. "Grant Opportunities, New Harvest". new-harvest.org. New Harvest. Archived from the original on October 18, 2016. Retrieved July 25, 2018.
115. "Home". New Harvest.
116. "Industrializing Cultivated Meats & Seafood Summit". 4th Industrializing Cultivated Meats & Seafood Summit.
117. "International Conference on Cultured Meat". International Conference on Cultured Meat. Retrieved 2020-01-02.
118. "Good Food Conference 2018". goodfoodconference.com.
119. "Cultured Meat Symposium Announces Cell-Based Meat Conference Planned for November 2018". KULR8.
120. "CMS21 – Cultured Meat Symposium". CMS21.
121. "The KET Maps — FoodTech Industry Landscapes". The KindEarth.Tech Maps.
122. "New Food Conference". www.new-food-conference.com.
123. "Clean Meat - The Bestselling Book by Paul Shapiro". cleanmeat.com.
124. Shapiro, Paul (2 January 2018). Clean Meat. ISBN   9781501189081.
125. Cultured Meat Future Food (8 April 2018). "Cultured Meat and Future Food Podcast Episode 03: Paul Shapiro" – via YouTube.
126. Wurgaft, Benjamin Aldes (September 2019). Meat Planet. ISBN   9780520295537.
127. Green Queen Media (30 July 2021). "This Children's Book Wants To Inspire Future Cell-Based Meat Makers".
128. The Spoon (31 July 2021). "Food Tech News: Food Waste Sneakers, Cell-Ag Children's Book, and Bon Appétit's New App".
129. "Alex Shirazi | User Experience Designer". alexshirazi.com. Retrieved 2021-08-15.
130. "Cultured Meat and Future Food". cleanmeatpodcast.com.
131. "Cultured Meat Future Food". YouTube.
132. "Spider silk made by photosynthetic bacteria". phys.org. Archived from the original on 7 August 2020. Retrieved 16 August 2020.
133. Foong, Choon Pin; Higuchi-Takeuchi, Mieko; Malay, Ali D.; Oktaviani, Nur Alia; Thagun, Chonprakun; Numata, Keiji (2020-07-08). "A marine photosynthetic microbial cell factory as a platform for spider silk production". Communications Biology. 3 (1). Springer Science and Business Media LLC: 357. doi:10.1038/s42003-020-1099-6. ISSN   2399-3642. PMC   7343832 . PMID   32641733.
134. Yirka, Bob (June 22, 2021). "Growing food with air and solar power: More efficient than planting crops". Phys.org. Retrieved 11 July 2021.
135. Leger, Dorian; Matassa, Silvio; Noor, Elad; Shepon, Alon; Milo, Ron; Bar-Even, Arren (29 June 2021). "Photovoltaic-driven microbial protein production can use land and sunlight more efficiently than conventional crops". Proceedings of the National Academy of Sciences. 118 (26): e2015025118. Bibcode:2021PNAS..11815025L. doi: 10.1073/pnas.2015025118 . ISSN   0027-8424. PMC   8255800 . PMID   34155098. S2CID   235595143.
136. "'Vegan spider silk' provides sustainable alternative to single-use plastics". phys.org. June 10, 2021. Retrieved 11 July 2021.
137. Kamada, Ayaka; Rodriguez-Garcia, Marc; Ruggeri, Francesco Simone; Shen, Yi; Levin, Aviad; Knowles, Tuomas P. J. (10 June 2021). "Controlled self-assembly of plant proteins into high-performance multifunctional nanostructured films". Nature Communications. 12 (1): 3529. Bibcode:2021NatCo..12.3529K. doi:10.1038/s41467-021-23813-6. ISSN   2041-1723. PMC   8192951 . PMID   34112802.

External links

• Overview of relevant bibliography
• New Harvest
• Cellular Agriculture Society

Further reading

• Clean meat, consumer attitudes and the transition to a cellular agriculture food economy
• A Closer Look at Cellular Agriculture and the Processes Defining It
• As lab-grown meat advances, U.S. lawmakers call for regulation
• CELLULAR AGRICULTURE: A WAY TO FEED TOMORROW'S SMART CITY? Archived 2019-05-28 at the Wayback Machine
• Cellular Agriculture, Intentional Imperfection And 'Post Truth': The Transformative Food Trends Of 2017
• The 4 Key Biotechnologies Needed to Get Cellular Agriculture to Commercialization
• Cellular agriculture: Growing meat in a lab setting
• How Might Cellular Agriculture Impact the Livestock, Dairy, and Poultry Industries?