Chondroitin-sulfate-ABC endolyase

Chondroitin-sulfate-ABC endolyase
Identifiers
EC no.	4.2.2.20
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The enzyme chondroitin-sulfate-ABC endolyase (EC 4.2.2.20) catalyzes the following process:

Endolytic cleavage of (1→4)-β-galactosaminic bonds between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid to produce a mixture of Δ⁴-unsaturated oligosaccharides of different sizes that are ultimately degraded to Δ⁴-unsaturated tetra- and disaccharides

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin-sulfate-ABC endolyase. Other names in common use include chondroitinase (ambiguous), chondroitin ABC eliminase (ambiguous), chondroitinase ABC (ambiguous), chondroitin ABC lyase (ambiguous), chondroitin sulfate ABC lyase (ambiguous), ChS ABC lyase (ambiguous), chondroitin sulfate ABC endoeliminase, chondroitin sulfate ABC endolyase, and ChS ABC lyase I.

References

• Yamagata T, Saito H, Habuchi O, Suzuki S (1968). "Purification and properties of bacterial chondroitinases and chondrosulfatases". J. Biol. Chem. 243 (7): 1523–35. PMID   5647268.
• Saito H, Yamagata T, Suzuki S (1968). "Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates". J. Biol. Chem. 243 (7): 1536–42. PMID   4231029.
• Suzuki S, Saito H, Yamagata T, Anno K, Seno N, Kawai Y, Furuhashi T (1968). "Formation of three types of disulfated disaccharides from chondroitin sulfates by chondroitinase digestion". J. Biol. Chem. 243 (7): 1543–50. PMID   5647269.
• Suzuki S; Hashimoto, N; Mochizuki, H; Kato, F; Makiguchi, Y; Horie, K; Suzuki, S (1997). "Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides". J. Biol. Chem. 272 (14): 9123–30. doi: 10.1074/jbc.272.14.9123 . PMID   9083041.
• Lauder RM; Nieduszynski, IA; Giannopoulos, M; Weeks, SD; Sadler, IH; Lauder, RM (2005). "Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides". FEBS J. 272 (24): 6276–86. doi: 10.1111/j.1742-4658.2005.05009.x . PMID   16336265.