Chondroitin B lyase

Chondroitin B lyase
Identifiers
EC no.	4.2.2.19
CAS no.	52227-83-5
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

The enzyme chondroitin B lyase (EC 4.2.2.19) catalyzes the following process:

Eliminative cleavage of dermatan sulfate containing (1→4)-β-D-hexosaminyl and (1→3)-β-D-glucurosonyl or (1→3)-α-L-iduronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groups to yield a 4,5-unsaturated dermatan-sulfate disaccharide (ΔUA-GalNAc-4S).

Glossary:

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is chondroitin B lyase. Other names in common use include chondroitinase B, ChonB, and ChnB.

References

• Gu K, Linhardt RJ, Laliberte M, Gu K, Zimmermann J (1995). "Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum". Biochem. J. 312 (Pt 2): 569–77. doi:10.1042/bj3120569. PMC   1136300 . PMID   8526872.
• Pojasek K, Raman R, Kiley P, Venkataraman G, Sasisekharan R (2002). "Biochemical characterization of the chondroitinase B active site". J. Biol. Chem. 277 (34): 31179–86. doi: 10.1074/jbc.M201552200 . PMID   12063249.
• Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R (2001). "Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum". Biochem. Biophys. Res. Commun. 286 (2): 343–51. doi:10.1006/bbrc.2001.5380. PMID   11500043.
• Suzuki K, Terasaki Y, Uyeda M (2002). "Inhibition of hyaluronidases and chondroitinases by fatty acids". J. Enzyme Inhib. Med. Chem. 17 (3): 183–6. doi:10.1080/14756360290032930. PMID   12443044.
• Ototani N, Yosizawa Z (1979). "Purification of chondroitinase B and chondroitinase C using glycosaminoglycan-bound AH-Sepharose 4B". Carbohydr. Res. 70 (2): 295–306. doi:10.1016/S0008-6215(00)87109-8. PMID   427837.
• K, Zimmermann JJ; Su H (2000). "Isolation and Expression in Escherichia coli of cslA and cslB, Genes Coding for the Chondroitin Sulfate-Degrading Enzymes Chondroitinase AC and Chondroitinase B, Respectively, from Flavobacterium heparinum". Appl. Environ. Microbiol. 66 (1): 29–35. Bibcode:2000ApEnM..66...29T. doi:10.1128/AEM.66.1.29-35.2000. PMC   91781 . PMID   10618199.
• V, Sasisekharan R, Cygler M (2004). "The structure of chondroitin B lyase complexed with glycosaminoglycan oligosaccharides unravels a calcium-dependent catalytic machinery". J. Biol. Chem. 279 (31): 32882–96. doi: 10.1074/jbc.M403421200 . PMC   4135467 . PMID   15155751.
• Li Y, Matte A, Su H, Cygler M (1999). "Crystallization and preliminary X-ray analysis of chondroitinase B from Flavobacterium heparinum". Acta Crystallogr. D. 55 (Pt 5): 1055–7. Bibcode:1999AcCrD..55.1055L. doi:10.1107/S0907444999002097. PMID   10216304.
• Huang W, Matte A, Li Y, Kim YS, Linhardt RJ, Su H, Cygler M (1999). "Crystal structure of chondroitinase B from Flavobacterium heparinum and its complex with a disaccharide product at 1.7 A resolution". J. Mol. Biol. 294 (5): 1257–69. doi:10.1006/jmbi.1999.3292. PMID   10600383.
• Lauder RM; Nieduszynski, IA; Giannopoulos, M; Weeks, SD; Sadler, IH; Lauder, RM (2005). "Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides". FEBS J. 272 (24): 6276–86. doi: 10.1111/j.1742-4658.2005.05009.x . PMID   16336265.