Germacrene A alcohol dehydrogenase

Germacrene A alcohol dehydrogenase
Identifiers
EC no.	1.1.1.314
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Germacrene A alcohol dehydrogenase (EC 1.1.1.314) is an enzyme with systematic name germacra-1(10),4,11(13)-trien-12-ol:NADP⁺ oxidoreductase. ^{[1] [2]} This enzyme catalyses the following chemical reaction

germacra-1(10),4,11(13)-trien-12-ol + 2 NADP⁺ + H₂O ${\displaystyle \rightleftharpoons }$ germacra-1(10),4,11(13)-trien-12-oate + 2 NADPH + 3 H⁺ (overall reaction)

(1a) germacra-1(10),4,11(13)-trien-12-ol + NADP⁺${\displaystyle \rightleftharpoons }$ germacra-1(10),4,11(13)-trien-12-al + NADPH + H⁺

(1b) germacra-1(10),4,11(13)-trien-12-al + NADP⁺ + H₂O ${\displaystyle \rightleftharpoons }$ germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H⁺

In Lactuca sativa this enzyme is a multifunctional enzyme with EC 1.14.13.123.

References

1. de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ (April 2001). "Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid dehydrogenase(s) involved in sesquiterpene lactone biosynthesis". Plant Physiology. 125 (4): 1930–40. doi:10.1104/pp.125.4.1930. PMC   88848 . PMID   11299372.
2. Nguyen DT, Göpfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK (May 2010). "Biochemical conservation and evolution of germacrene A oxidase in asteraceae". The Journal of Biological Chemistry. 285 (22): 16588–98. doi: 10.1074/jbc.M110.111757 . PMC   2878029 . PMID   20351109.

External links

• Germacrene+A+alcohol+dehydrogenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)