PRPF6

PRPF6
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3JCR
Identifiers
Aliases	PRPF6 , ANT-1, ANT1, C20orf14, Prp6, RP60, SNRNP102, TOM, U5-102K, hPrp6, pre-mRNA processing factor 6
External IDs	OMIM: 613979; MGI: 1922946; HomoloGene: 5368; GeneCards: PRPF6; OMA:PRPF6 - orthologs
Gene location (Human) Chr. Chromosome 20 (human) [1] Band 20q13.33 Start 63,981,132 bp [1] End 64,033,100 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 H4 Start 181,233,661 bp [2] End 181,297,453 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in tendon of biceps brachii paraflocculus of cerebellum beta cell right hemisphere of cerebellum ganglionic eminence right uterine tube ventricular zone skin of leg Achilles tendon popliteal artery Top expressed in supraoptic nucleus aortic valve ascending aorta Rostral migratory stream fossa condyle internal carotid artery external carotid artery zygote primary oocyte More reference expression data BioGPS n/a
Gene ontology Molecular function transcription coactivator activity protein binding androgen receptor binding RNA binding ribonucleoprotein complex binding Cellular component U4/U6 x U5 tri-snRNP complex catalytic step 2 spliceosome membrane U5 snRNP spliceosomal complex nucleus nucleoplasm nuclear speck U2-type precatalytic spliceosome Biological process mRNA splicing, via spliceosome RNA processing spliceosomal tri-snRNP complex assembly RNA splicing, via transesterification reactions mRNA processing spliceosomal complex assembly RNA localization positive regulation of transcription by RNA polymerase II RNA splicing Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 24148 68879 Ensembl ENSG00000101161 ENSMUSG00000002455 UniProt O94906 Q91YR7 RefSeq (mRNA) NM_012469 NM_133701 RefSeq (protein) NP_036601 NP_598462 Location (UCSC) Chr 20: 63.98 – 64.03 Mb Chr 2: 181.23 – 181.3 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Pre-mRNA-processing factor 6 is a protein that in humans is encoded by the PRPF6 gene. ^{[5] [6] [7]}

The protein encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing. ^[7]

Interactions

PRPF6 has been shown to interact with TXNL4B, ^[8] ARAF ^{[9] [10]} and Androgen receptor. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000101161 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000002455 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nishikimi A, Mukai J, Kioka N, Yamada M (January 2000). "A novel mammalian nuclear protein similar to Schizosaccharomyces pombe Prp1p/Zer1p and Saccharomyces cerevisiae Prp6p pre-mRNA splicing factors". Biochim Biophys Acta. 1435 (1–2): 147–52. doi:10.1016/s0167-4838(99)00203-4. PMID   10561546.
6. Makarov EM, Makarova OV, Achsel T, Luhrmann R (June 2000). "The human homologue of the yeast splicing factor prp6p contains multiple TPR elements and is stably associated with the U5 snRNP via protein-protein interactions". J Mol Biol. 298 (4): 567–75. doi:10.1006/jmbi.2000.3685. PMID   10788320.
7. "Entrez Gene: PRPF6 PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)".
8. Sun, Xiaojing; Zhang Hua; Wang Dan; Ma Dalong; Shen Yan; Shang Yongfeng (July 2004). "DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression". J. Biol. Chem. 279 (31). United States: 32839–47. doi: 10.1074/jbc.M402522200 . ISSN   0021-9258. PMID   15161931.
9. Yuryev, Anton; Wennogle Lawrence P (February 2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics. 81 (2). United States: 112–25. doi:10.1016/S0888-7543(02)00008-3. ISSN   0888-7543. PMID   12620389.
10. Yuryev, A; Ono M; Goff S A; Macaluso F; Wennogle L P (July 2000). "Isoform-specific localization of A-RAF in mitochondria". Mol. Cell. Biol. 20 (13). UNITED STATES: 4870–8. doi:10.1128/MCB.20.13.4870-4878.2000. ISSN   0270-7306. PMC   85938 . PMID   10848612.
11. Zhao, Yue; Goto Kiminobu; Saitoh Masayuki; Yanase Toshihiko; Nomura Masatoshi; Okabe Taijiro; Takayanagi Ryoichi; Nawata Hajime (August 2002). "Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactivator for the receptor". J. Biol. Chem. 277 (33). United States: 30031–9. doi: 10.1074/jbc.M203811200 . ISSN   0021-9258. PMID   12039962.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Yuryev A, Ono M, Goff SA, et al. (2000). "Isoform-specific localization of A-RAF in mitochondria". Mol. Cell. Biol. 20 (13): 4870–8. doi:10.1128/MCB.20.13.4870-4878.2000. PMC   85938 . PMID   10848612.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi: 10.1038/414865a . PMID   11780052.
• Jurica MS, Licklider LJ, Gygi SR, et al. (2002). "Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis". RNA. 8 (4) S1355838202021088: 426–39. doi:10.1017/S1355838202021088. PMC   1370266 . PMID   11991638.
• Zhao Y, Goto K, Saitoh M, et al. (2002). "Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactivator for the receptor". J. Biol. Chem. 277 (33): 30031–9. doi: 10.1074/jbc.M203811200 . PMID   12039962.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Yuryev A, Wennogle LP (2003). "Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis". Genomics. 81 (2): 112–25. doi:10.1016/S0888-7543(02)00008-3. PMID   12620389.
• Goto K, Zhao Y, Saito M, et al. (2003). "Activation function-1 domain of androgen receptor contributes to the interaction between two distinct subnuclear compartments". J. Steroid Biochem. Mol. Biol. 85 (2–5): 201–8. doi:10.1016/S0960-0760(03)00196-1. PMID   12943705. S2CID   46683850.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Will CL, Schneider C, Hossbach M, et al. (2004). "The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome". RNA. 10 (6): 929–41. doi:10.1261/rna.7320604. PMC   1370585 . PMID   15146077.
• Sun X, Zhang H, Wang D, et al. (2004). "DLP, a novel Dim1 family protein implicated in pre-mRNA splicing and cell cycle progression". J. Biol. Chem. 279 (31): 32839–47. doi: 10.1074/jbc.M402522200 . PMID   15161931.
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC   515316 . PMID   15342556.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Laggerbauer B, Liu S, Makarov E, et al. (2005). "The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation". RNA. 11 (5): 598–608. doi:10.1261/rna.2300805. PMC   1370748 . PMID   15840814.
• Batsché E, Yaniv M, Muchardt C (2006). "The human SWI/SNF subunit Brm is a regulator of alternative splicing". Nat. Struct. Mol. Biol. 13 (1): 22–9. doi:10.1038/nsmb1030. PMID   16341228. S2CID   11587161.
• Fan S, Goto K, Chen G, et al. (2006). "Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor". Biochem. Biophys. Res. Commun. 341 (1): 192–201. Bibcode:2006BBRC..341..192F. doi:10.1016/j.bbrc.2005.12.167. PMID   16414017.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC   1847948 . PMID   17353931.

External links

• GeneReviews/NCBI/NIH/UW entry on Retinitis Pigmentosa Overview