(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase

(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase
Identifiers
EC no.	1.3.1.53
CAS no.	162032-77-1
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase (EC 1.3.1.53) is an enzyme that catalyzes the chemical reaction

3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylic acid

+ NAD⁺

 

 

 

CO₂ + H⁺

 

CO₂ + H⁺

 

protocatechuic acid

+ NADH

 

The two substrates of this enzyme are (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate and oxidised nicotinamide adenine dinucleotide (NAD⁺). Its products are protocatechuic acid, carbon dioxide, reduced NADH and a proton. ^{[1] [2]}

This enzyme is a part of the terephthalate degradation pathway in bacteria. ^[3]

Family

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate:NAD+ oxidoreductase. Another name in common use is (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase. This enzyme uses ferrous iron as a cofactor. ^[1]

References

1. Enzyme 1.3.1.53 at KEGG Pathway Database.
2. Saller E, Laue HR, Schlafli Oppenberg HR, Cook AM (1996). "Purification and some properties of (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2". FEMS Microbiol. Lett. 130: 97–102. doi: 10.1111/j.1574-6968.1995.tb07705.x .
3. National Center for Biotechnology Information. "PubChem Enzyme Summary for Enzyme 1.3.1.53". pubchem.ncbi.nlm.nih.gov. Archived from the original on 2020-09-15. Retrieved 2020-07-30.