15-oxoprostaglandin 13-oxidase

15-oxoprostaglandin 13-oxidase
Identifiers
EC no.	1.3.1.48
CAS no.	57406-74-3
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, 15-oxoprostaglandin 13-oxidase (EC 1.3.1.48) is an enzyme that catalyzes the chemical reaction

15-oxodihydroprostaglandin E1

+ NAD⁺

 

 

 

H⁺

 

H⁺

 

15-oxoprostaglandin E1

+ NADH

 

The two substrates of this enzyme are 15-oxodihydroprostaglandin E1 and oxidised nicotinamide adenine dinucleotide (NAD⁺). Its products are 15-oxoprostaglandin E1, reduced NADH, and a proton. The enzyme can also use nicotinamide adenine dinucleotide phosphate as an alternative cofactor. ^{[1] [2] [3]}

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (5Z)-(15S)-11alpha-hydroxy-9,15-dioxoprostanoate:NAD(P)+ Delta13-oxidoreductase. Other names in common use include 15-oxo-Delta13-prostaglandin reductase, Delta13-15-ketoprostaglandin reductase, 15-ketoprostaglandin Delta13-reductase, prostaglandin Delta13-reductase, prostaglandin 13-reductase, and 15-ketoprostaglandin Delta13-reductase.

Structural studies

As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1V3T, 1V3U, 1V3V, and 2DM6.

References

1. Enzyme 1.3.1.48 at KEGG Pathway Database.
2. Hansen HS (1982). "Purification and assay of 15-ketoprostaglandin Δ13-reductase from bovine lung". Prostaglandins and Arachidonate Metabolites. Methods in Enzymology. Vol. 86. pp. 156–63. doi:10.1016/0076-6879(82)86186-7. ISBN   978-0-12-181986-6. PMID   6290839.
3. Jarabak J (1982). "Isolation and properties of a 15-ketoprostaglandin Δ13-reductase from human placenta". Prostaglandins and Arachidonate Metabolites. Methods in Enzymology. Vol. 86. pp. 163–7. doi:10.1016/0076-6879(82)86187-9. ISBN   978-0-12-181986-6. PMID   7132753.