2,5-diketocamphane 1,2-monooxygenase

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2,5-diketocamphane 1,2-monooxygenase
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EC no. 1.14.14.108
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2,5-diketocamphane 1,2-monooxygenase (EC 1.14.14.108, 2,5-diketocamphane lactonizing enzyme, ketolactonase I, 2,5-diketocamphane 1,2-monooxygenase oxygenating component, 2,5-DKCMO, camphor 1,2-monooxygenase, camphor ketolactonase I) is an enzyme with systematic name (+)-bornane-2,5-dione,NADH:oxygen oxidoreductase (1,2-lactonizing). [1] [2] [3] [4] [5] [6] This enzyme catalyses the following chemical reaction

(+)-bornane-2,5-dione + O2 + NADH + H+ (+)-5-oxo-1,2-campholide + NAD+ + H2O

2,5-diketocamphane 1,2-monooxygenase is a flavoprotein (FMN) which requires Fe2+.

Related Research Articles

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<span class="mw-page-title-main">4-Methylcatechol</span> Chemical compound

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References

  1. ↑ Conrad HE, Dubus R, Namtvedt MJ, Gunsalus IC (January 1965). "Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton". The Journal of Biological Chemistry. 240: 495–503. PMID   14253460.
  2. ↑ Yu CA, Gunsalus IC (November 1969). "Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components". The Journal of Biological Chemistry. 244 (22): 6149–52. PMID   4310834.
  3. ↑ Taylor DG, Trudgill PW (February 1986). "Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453". Journal of Bacteriology. 165 (2): 489–97. PMC   214445 . PMID   3944058.
  4. ↑ Selifonov SA (August 1992). "Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid". Biochemical and Biophysical Research Communications. 186 (3): 1429–36. doi:10.1016/s0006-291x(05)81566-9. PMID   1510672.
  5. ↑ Jones KH, Smith RT, Trudgill PW (April 1993). "Diketocamphane enantiomer-specific 'Baeyer-Villiger' monooxygenases from camphor-grown Pseudomonas putida ATCC 17453". Journal of General Microbiology. 139 (4): 797–805. doi: 10.1099/00221287-139-4-797 . PMID   8515237.
  6. ↑ Kadow M, Saß S, Schmidt M, Bornscheuer UT (June 2011). "Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007". AMB Express. 1 (1): 13. doi:10.1186/2191-0855-1-13. PMC   3222318 . PMID   21906366.
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