Aristolochene synthase

aristolochene synthase
Identifiers
EC no.	4.2.3.9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The enzyme aristolochene synthase (EC 4.2.3.9) catalyzes the chemical reaction

(2E,6E)-farnesyl diphosphate ⇌ (+)-aristolochene + diphosphate

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is (2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, aristolochene-forming). Other names in common use include sesquiterpene cyclase, trans,trans-farnesyl diphosphate aristolochene-lyase, trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing and aristolochene-forming). This enzyme participates in terpenoid biosynthesis.

This protein may use the morpheein model of allosteric regulation. ^[1]

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2E4O and 2OA6. They are both notable for the very high helix content of the structure.

References

1. T. Selwood & E. K. Jaffe (2011). "Dynamic dissociating homo-oligomers and the control of protein function". Arch. Biochem. Biophys. 519 (2): 131–43. doi:10.1016/j.abb.2011.11.020. PMC   3298769 . PMID   22182754.

• Cane DE, Prabhakaran PC, Oliver JS, McIlwaine DB (1990). "Aristolochene biosynthesis. Stereochemistry of the deprotonation steps in the enzymatic cyclization of farnesyl pyrophosphate". J. Am. Chem. Soc. 112 (8): 3209–3210. doi:10.1021/ja00164a051.
• Noguchi H, Rawlings BJ (1989). "Aristolochene biosynthesis and enzymatic cyclization of farnesyl pyrophosphate". J. Am. Chem. Soc. 111 (24): 8914–8916. doi:10.1021/ja00206a022.
• Hohn TM, Plattner RD (1989). "Purification and characterization of the sesquiterpene cyclase aristolochene synthase from Penicillium roqueforti". Arch. Biochem. Biophys. 272 (1): 137–43. doi:10.1016/0003-9861(89)90204-X. PMID   2544140.
• Proctor RH, Hohn TM (1993). "Aristolochene synthase. Isolation, characterization, and bacterial expression of a sesquiterpenoid biosynthetic gene (Ari1) from Penicillium roqueforti". J. Biol. Chem. 268 (6): 4543–8. doi: 10.1016/S0021-9258(18)53644-9 . PMID   8440737.
• Calvert MJ, Ashton PR, Allemann RK (2002). "Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene". J. Am. Chem. Soc. 124 (39): 11636–41. doi:10.1021/ja020762p. PMID   12296728.