Botryococcene synthase

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Botryococcene synthase
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EC no. 1.3.1.97
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Botryococcene synthase (EC 1.3.1.97, SSL-3 (gene)) is an enzyme with systematic name C30 botryococcene:NADP+ oxidoreductase . [1] This enzyme catalyses the following chemical reaction

C30 botryococcene + NADP + + diphosphate presqualene diphosphate + NADPH + H+

This enzyme is isolated from the green alga Botryococcus braunii BOT22.

Related Research Articles

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Botryococcus braunii is a green, pyramid-shaped planktonic microalga that is of potentially great importance in the field of biotechnology. Colonies held together by a lipid biofilm matrix can be found in temperate or tropical oligotrophic lakes and estuaries, and will bloom when in the presence of elevated levels of dissolved inorganic phosphorus. The species is notable for its ability to produce high amounts of hydrocarbons, especially oils in the form of Triterpenes, that are typically around 30–40% of their dry weight. Compared to other green alge species it has a relatively thick cell wall that is accumulated from previous cellular divisions; making extraction of cytoplasmic components rather difficult. Much of the useful hydrocarbon oil is outside of the cell.

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References

  1. Niehaus TD, Okada S, Devarenne TP, Watt DS, Sviripa V, Chappell J (July 2011). "Identification of unique mechanisms for triterpene biosynthesis in Botryococcus braunii". Proceedings of the National Academy of Sciences of the United States of America. 108 (30): 12260–5. Bibcode:2011PNAS..10812260N. doi: 10.1073/pnas.1106222108 . PMC   3145686 . PMID   21746901.