Cob(I)yrinic acid a,c-diamide adenosyltransferase

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ATP:corrinoid adenosyltransferase
Protein MMAB PDB 2idx.png
Human mitochondrial Cob(I)yrinic acid a,c-diamide adenosyltransferase, MMAB. [1]
Identifiers
EC no. 2.5.1.17
CAS no. 37277-84-2
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BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
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Gene Ontology AmiGO / QuickGO
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ATP:corrinoid adenosyltransferase
PDB 1g5t EBI.jpg
the three-dimensional structure of atp:corrinoid adenosyltransferase from salmonella typhimurium. apo-atp form
Identifiers
SymbolCobA_CobO_BtuR
Pfam PF02572
Pfam clan CL0023
InterPro IPR003724
SCOP2 1g64 / SCOPe / SUPFAM
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary
Cobalamin adenosyltransferase (PduO/EutT)
PDB 1nog EBI.jpg
crystal structure of conserved protein 0546 from thermoplasma acidophilum
Identifiers
SymbolCob_adeno_trans
Pfam PF01923
InterPro IPR002779
SCOP2 1nog / SCOPe / SUPFAM
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

In molecular biology, cob(I)yrinic acid a,c-diamide adenosyltransferase (also known as ATP:cob(I)alamin adenosyltransferase or ATP:corrinoid adenosyltransferase) EC 2.5.1.17 is an enzyme which catalyses the conversion of cobalamin (vitamin B12) into one of its coenzyme forms, adenosylcobalamin (coenzyme B12, AdoCbl). [2] [3] Adenosylcobalamin is required as a cofactor for the activity of certain enzymes. AdoCbl contains an adenosyl moiety liganded to the cobalt ion of cobalamin via a covalent Co-C bond.

Contents

ATP:cob(I)alamin adenosyltransferases are classed into three groups: CobA-type, [4] EutT-type [5] and PduO-type. [6] Each of the three enzyme types appears to be specialised for particular AdoCbl-dependent enzymes or for the de novo synthesis of AdoCbl. PduO and EutT are distantly related, sharing short conserved motifs, while CobA is evolutionarily unrelated and is an example of convergent evolution.

The CobA group includes the ATP:cob(I)alamin adenosyltransferases CobA ( Salmonella typhimurium ), CobO ( Pseudomonas denitrificans), and ButR (Escherichia coli). There is a high degree of sequence identity between these proteins. [7] CobA is responsible for attaching the adenosyl moiety from ATP to the cobalt ion of the corrin ring, necessary for the conversion of cobalamin to adenosylcobalamin. [3] [4] PduO functions to convert cobalamin to AdoCbl for 1,2-propanediol degradation, [8] while EutT produces AdoCbl for ethanolamine utilisation. [9]

Synonyms

This enzyme is also known as:

See also

Related Research Articles

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<span class="mw-page-title-main">1-Aminopropan-2-ol</span> Chemical compound

1-Aminopropan-2-ol is the organic compound with the formula CH3CH(OH)CH2NH2. It is an amino alcohol. The term isopropanolamine may also refer more generally to the additional homologs diisopropanolamine (DIPA) and triisopropanolamine (TIPA).

<span class="mw-page-title-main">Precorrin-6A synthase (deacetylating)</span>

In enzymology, precorrin-6A synthase (deacetylating) (EC 2.1.1.152) is an enzyme that catalyzes the chemical reaction

In enzymology, a precorrin-6A reductase (EC 1.3.1.54) is an enzyme that catalyzes the chemical reaction

In enzymology, a precorrin-3B synthase (EC 1.14.13.83) is an enzyme that catalyzes the chemical reaction

<span class="mw-page-title-main">Cob(II)yrinic acid a,c-diamide reductase</span>

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<span class="mw-page-title-main">Cobalt chelatase</span> Enzyme

Cobalt chelatase (EC 6.6.1.2) is an enzyme that catalyzes the chemical reaction

In enzymology, a hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) (EC 6.3.5.9) is an enzyme that catalyzes the chemical reaction

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<span class="mw-page-title-main">Nicotinate-nucleotide—dimethylbenzimidazole phosphoribosyltransferase</span>

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<span class="mw-page-title-main">Adenosylcobinamide-phosphate guanylyltransferase</span> Class of enzymes

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<span class="mw-page-title-main">MMAB</span> Protein-coding gene in the species Homo sapiens

Cob(I)yrinic acid a,c-diamide adenosyltransferase, mitochondrial is an enzyme that in humans is encoded by the MMAB gene.

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<span class="mw-page-title-main">Cobalamin biosynthesis</span>

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Adenosylcobinamide-phosphate synthase is an enzyme with systematic name adenosylcobyric acid:(R)-1-aminopropan-2-yl phosphate ligase (ADP-forming). This enzyme catalyses the following chemical reaction

References

  1. Schubert HL, Hill CP (December 2006). "Structure of ATP-bound human ATP:cobalamin adenosyltransferase". Biochemistry. 45 (51): 15188–96. doi:10.1021/bi061396f. PMC   2532598 . PMID   17176040.
  2. Mera PE, Escalante-Semerena JC (September 2010). "Multiple roles of ATP:cob(I)alamin adenosyltransferases in the conversion of B12 to coenzyme B12". Appl. Microbiol. Biotechnol. 88 (1): 41–8. doi:10.1007/s00253-010-2773-2. PMC   3034633 . PMID   20677021.
  3. 1 2 Sheppard DE, Penrod JT, Bobik T, Kofoid E, Roth JR (November 2004). "Evidence that a B12-adenosyl transferase is encoded within the ethanolamine operon of Salmonella enterica". J. Bacteriol. 186 (22): 7635–44. doi:10.1128/JB.186.22.7635-7644.2004. PMC   524904 . PMID   15516577.
  4. 1 2 Buan NR, Rehfeld K, Escalante-Semerena JC (May 2006). "Studies of the CobA-type ATP:Co(I)rrinoid adenosyltransferase enzyme of Methanosarcina mazei strain Go1". J. Bacteriol. 188 (10): 3543–50. doi:10.1128/JB.188.10.3543-3550.2006. PMC   1482872 . PMID   16672609.
  5. Buan NR, Suh SJ, Escalante-Semerena JC (September 2004). "The eutT gene of Salmonella enterica Encodes an oxygen-labile, metal-containing ATP:corrinoid adenosyltransferase enzyme". J. Bacteriol. 186 (17): 5708–14. doi:10.1128/JB.186.17.5708-5714.2004. PMC   516830 . PMID   15317775.
  6. Johnson CL, Pechonick E, Park SD, Havemann GD, Leal NA, Bobik TA (March 2001). "Functional genomic, biochemical, and genetic characterization of the Salmonella pduO gene, an ATP:cob(I)alamin adenosyltransferase gene". J. Bacteriol. 183 (5): 1577–84. doi:10.1128/JB.183.5.1577-1584.2001. PMC   95042 . PMID   11160088.
  7. Suh SJ, Escalante-Semerena JC (July 1993). "Cloning, sequencing and overexpression of cobA which encodes ATP:corrinoid adenosyltransferase in Salmonella typhimurium". Gene. 129 (1): 93–7. doi:10.1016/0378-1119(93)90701-4. PMID   7916712.
  8. Luers F, Seyfried M, Daniel R, Gottschalk G (September 1997). "Glycerol conversion to 1,3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase". FEMS Microbiol. Lett. 154 (2): 337–45. doi: 10.1016/s0378-1097(97)00351-0 . PMID   9311132.
  9. Buan NR, Escalante-Semerena JC (June 2006). "Purification and initial biochemical characterization of ATP:Cob(I)alamin adenosyltransferase (EutT) enzyme of Salmonella enterica". J. Biol. Chem. 281 (25): 16971–7. doi: 10.1074/jbc.M603069200 . PMID   16636051.
This article incorporates text from the public domain Pfam and InterPro: IPR003724
This article incorporates text from the public domain Pfam and InterPro: IPR002779