Germacradienol synthase

Germacradienol synthase
Identifiers
EC no.	4.2.3.22
CAS no.	211049-88-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Germacradienol synthase (EC 4.2.3.22, germacradienol/germacrene-D synthase, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase [(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming]) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming). ^{[1] [2] [3]} This enzyme catalyses the following chemical reaction

(2E,6E)-farnesyl diphosphate + H₂O ${\displaystyle \rightleftharpoons }$ (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate

This enzyme requires Mg²⁺ for activity.

References

1. Cane DE, Watt RM (February 2003). "Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1547–51. Bibcode:2003PNAS..100.1547C. doi: 10.1073/pnas.0337625100 . PMC   149869 . PMID   12556563.
2. He X, Cane DE (March 2004). "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)". Journal of the American Chemical Society. 126 (9): 2678–9. Bibcode:2004JAChS.126.2678H. doi:10.1021/ja039929k. PMID   14995166.
3. Gust B, Challis GL, Fowler K, Kieser T, Chater KF (February 2003). "PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin". Proceedings of the National Academy of Sciences of the United States of America. 100 (4): 1541–6. Bibcode:2003PNAS..100.1541G. doi: 10.1073/pnas.0337542100 . PMC   149868 . PMID   12563033.

External links

• Germacradienol+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)