Glossina fuscipes

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Glossina fuscipes
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Diptera
Family: Glossinidae
Genus: Glossina
Species:
G. fuscipes
Binomial name
Glossina fuscipes
Newstead, 1910
Subspecies
Synonyms
  • Glossina angolensis Vanderplank, 1948
  • Glossina angolensis Vanderplank, 1949
  • Glossina martinii Zumpt, 1935
  • Glossina quanzensis Pires, 1948
  • Glossina ziemanni Grunberg, 1912

Glossina fuscipes is a riverine fly species in the genus Glossina , which are commonly known as tsetse flies. [1] Typically found in sub-Saharan Africa but with a small Arabian range, [2] G. fuscipes is a regional vector of African trypanosomiasis, commonly known as sleeping sickness, that causes significant rates of morbidity and mortality among humans and livestock. [3] Consequently, the species is among several being targeted by researchers for population control as a method for controlling the disease. [4]

Contents

Physical description

G. fuscipes are often brown or grey-brown in color. Their bodies tend to have varied dark and light patches, effectively camouflaging them on surfaces such as bark, rock, or soil. At rest, G. fuscipes appear slim as they fold their wings on their backs so that one lies on top of the other. This is in contrast to houseflies and blowflies whose wings project outward at an angle while resting on their backs. Following a blood meal, the insect's abdomen will appear large, rounded and red. [5]

Males

When the male G. fuscipes is examined from the ventral side, a rounded structure named the hypopygium can be seen at the posterior end of the abdomen. Immediately in front of the hypopygium is a plate bearing dark hairs called hectors. Both the hypopygium and the hectors help distinguish male from females and serve to grasp onto the end of the female abdomen during mating. As copulation commences, the hypopygium unfolds to uncover superior and inferior claspers as well as the aedeagus. [5]

Females

The end of the female abdomen lacks any significant structures that would be the counterpart of the male hypopygium and hectors; however, females display a vulva, which can exhibit several small plates that aid in species identification. [5]

Life cycle

Egg stage

In a few hours, the sperm move from the spermatophore into the spermatheca, where they remain active for the remainder of the female's life. Eggs are fertilized immediately as they enter the uterus by sperm from the spermatheca that come into contact and penetrate the anterior portion of the egg. The fertilized egg remains in the uterus for about four days as the instar larva begins to develop. [5]

Once she has mated, a female can produce larvae for the remainder of her life. At about 25 °C, a female fly will produce mature larva every 9–10 days with the exception of the first, which may take up to 18–20 days. Lower temperatures result in a lower rate of breeding whereas higher temperatures increase the rate of breeding. Temperatures either too high or too low may cease breeding altogether. [6]

Larva

The G. fuscipes larva in passes through three instars as it grows up to when the fully grown larva is dropped by a female fly. The larva has a mouth at the anterior end and two spiracles at the posterior end. Rather unusually, the larva spends most of its time and does all its feeding within the mother's body.[ citation needed ]

Apart from food stored in the egg, the food supply for the three larval instar stages comes from the mother's milk gland. The milky secretions of this gland are expelled out of the gland duct at the head end of the larva. The larva sucks up the milky secretion and passes it directly to the midgut where it is slowly digested and assimilated.[ citation needed ]

For air supply, the larva depends on air entering the vulva of the female. The air must pass into the female's posterior spiracles or polypneustic lobes to reach the larva. [5]

Abortion

If a larva fails to reach its full size, it will be prematurely expelled from the uterus. The aborted larva dies. Abortions could be due to the mother fly not obtaining enough food or also when carelessly handled or exposed to insecticide. Eggs are subjected to abortion for the same reasons. [5]

Pupa

The pupa is a dark brown, shorter than the larva that produced it, and rounded with polypneustic lobes at the posterior end. The lobes are distinctively shaped and can help to distinguish the G. fuscipes pupa from that of other flies. The pupa also has a hard case on its outside called the puparium.[ citation needed ]

The pupal stage lasts about four to five weeks according to temperature. Higher temperatures shorten the pupal period. In contrast, lower temperatures lengthen the pupal period to more than 50 days in certain climates. However, temperature extremes will cause death. [5]

Adult

When ready to emerge, the young adult fly expands its ptilinum to burst open puparium's end. Out of the fresh hole and surrounding soil, the adult emerges by using the ptilinum, struggling to the top of the soil and into open air. At this stage, the adult's body is very soft while its wings are small and crumpled. After a few urinations, the wings will expand towards their proper size. From the time between the emergence of the fly and its first meal, the adult is called a teneral fly. After the first blood meal has been taken, the fly is then termed a non-teneral fly. [5]

Reproduction

Mating

During mating, males settle on the back of the female. Claspers at the posterior end of the male abdomen unfold in order to grip the end of the female abdomen. This mating position may be maintained for an hour or two before the duo parts.[ citation needed ]

Females typically mate a young age, either before or around the same time of their first blood meals. Females usually mate only once in their lives though it is possible mate more than once, whereas males tend to mate several times. Older males are more likely to mate successfully than very young males.[ citation needed ]

During mating, the aedeagus is inserted into the vulva and reaches into the uterus as far as the spermatheca exit. A sizable ball of sperm is deposited there in the form of a spermatophore. At the conclusion of mating, the male releases his grip on the female before flying away. [5]

Distribution and habitat

G. fuscipes are found in sub-Saharan African and a subpopulation of G. f. fuscipes exists in the very southern part of the Arabian peninsula. G. f. f. and G. m. submorsitans are the only subspecies of Glossina which survive outside Africa, including in southwestern Saudi Arabia. [2] They prefer high-humidity areas, namely biomes such as mangrove swamps, rain forests, lake shores, and gallery forests along rivers. G. fuscipes occupies a large inland block centered on Democratic Republic of the Congo but also covering land surrounding countries in addition to Gabon, Cameroon and a southern portion of Chad. [5]

Evolution and taxonomy

The genus Glossina is regarded as an isolated genus and it is usually classified into its own family Glossinidae. The genus is further divided into three subgenera, Morsitans, Fusca, and Palpalis, the latter of which being the subgenus to which G. fuscipes belongs. The species is further broken down into subspecies G. f. fuscipes , G. f. martinii , and G. f. quanzensis . [5]

Food Resources

G. fuscipes feed on vertebrate blood and have been traditionally described as strictly hematophagous. Glucose sugars are not a metabolic requirement for this species because it uses a proline-alanine shuttle system for the distribution of energy. Instead, triglycerides are used for storage in fly body fat and milk secretions. However, researchers have conducted laboratory experiments and a field study that show G. fuscipes are able to feed on sugar water in the lab and wild flies contain sugar residues. Although continuous feeding with high sugar concentrations appeared to be toxic, sugar given either occasionally or at low concentrations did not affect mortality and fecundity. [7]

Predators

G. fuscipes adults and pupae are a food source for a variety of predators including vertebrates and arthropods. However, no insectivorous species is known to solely feed on G. fuscipes or tsetse flies in general. Thus, a reduction in insectivorous birds during general tsetse fly control campaigns could be attributed to the simultaneous insecticide-related removal of other insect species than decreases in tsetse flies themselves. [8]

Trypanosomiasis

Some trypanosome species, transmitted by G. fuscipes and other tsetse fly species, cause the infectious disease trypanosomiasis. In humans, G. fuscipes trypanosomiasis is also known as sleeping sickness. In animals, the disease may be known as nagana or surra according to the animal species infected as well as the trypanosome species involved. Nagana typically refers to the disease specifically in cattle and horses; however, it is commonly used to describe any type of animal trypanosomiasis. [9]

Disease vectors and hosts

G. fuscipes, alongside other tsetse flies, are prominent biological vectors of protozoan parasites belonging to the genus Trypanosoma known to cause the namesake diseases in various vertebrate species including humans, antelopes, bovine cattle, camels, horses, sheep, goats, and pigs. The parasites are transmitted to humans via bites from G. fuscipes, which have acquired their infection from other human beings or animals harboring human-pathogenic parasites. [9]

The table below summarizes this information for the G. fuscipes species; however, the diseases listed below may be transferred by other tsetse fly species in addition to G. fuscipes.

DiseaseSpecies affectedTrypanosoma agentsDistribution
Sleeping sickness — acute formhumansT. brucei rhodesienseEastern Africa
Nagana — acute formantelope
cattle
camels
horses		 T. brucei brucei Africa
Nagana — acute formdomestic pigs
cattle
camels
horses		 T. simiae Africa
Surra — chronic formdomestic pigs
warthog (Phacochoerus aethiopicus)
forest hogs ( Hylochoerus spp.)		 T. suis Africa

Population control

The containment of sleeping sickness and nagana would be of great benefit to rural communities in sub-Saharan Africa, alleviating poverty and improving food security, thus efforts are undertaken in rein in local populations of G. fuscipes via methods such as pesticide campaigns, trapping, or the sterile insect technique. [10]

Mutualism

Microbiome

G. fuscipes flies rely on the obligate symbiont bacterial genus Wigglesworthia to supplement their diets with nutrients essential for fecundity. [11] The adult immune system relies similarly on Wigglesworthia for activation and development. [12] A secondary, facultative symbiont is the genus Sodalis , which is present in tsetse populations considered to play a role in the ability to transmit trypanosomes. [13] Finally, the third symbiont is the genus Wolbachia , transovarially transmitted between generations. To enhance transmission and survival, Wolbachia has evolved mechanisms to alter host reproduction. [14]

Using both culture-dependent and independent methods, it was shown that Kenyan populations of the subspecies G. f. fuscipes harbor diverse range of bacteria. Of the flies tested, bacteria were isolated from 72% of the sample population with 23 bacterial species identified. Of these, the Bacillota phylum constituted 16 species, seven of which belong to the genus Bacillus . [15]

See also

Related Research Articles

<span class="mw-page-title-main">African trypanosomiasis</span> Parasitic disease

African trypanosomiasis, also known as African sleeping sickness or simply sleeping sickness, is an insect-borne parasitic infection of humans and other animals. It is caused by the species Trypanosoma brucei. Humans are infected by two types, Trypanosoma brucei gambiense (TbG) and Trypanosoma brucei rhodesiense (TbR). TbG causes over 92% of reported cases. Both are usually transmitted by the bite of an infected tsetse fly and are most common in rural areas.

<span class="mw-page-title-main">Trypanosomatida</span> Flagellate kinetoplastid excavate order

Trypanosomatida is a group of kinetoplastid unicellular organisms distinguished by having only a single flagellum. The name is derived from the Greek trypano (borer) and soma (body) because of the corkscrew-like motion of some trypanosomatid species. All members are exclusively parasitic, found primarily in insects. A few genera have life-cycles involving a secondary host, which may be a vertebrate, invertebrate or plant. These include several species that cause major diseases in humans. Some trypanosomatida are intracellular parasites, with the important exception of Trypanosoma brucei.

<span class="mw-page-title-main">Tsetse fly</span> Genus of disease-spreading insects

Tsetse are large, biting flies that inhabit much of tropical Africa. Tsetse flies include all the species in the genus Glossina, which are placed in their own family, Glossinidae. The tsetse is an obligate parasite, which lives by feeding on the blood of vertebrate animals. Tsetse has been extensively studied, because of their role in transmitting disease. They have a prominent economic impact in sub-Saharan Africa, as the biological vectors of trypanosomes, causing human and animal trypanosomiasis.

<span class="mw-page-title-main">Trypanosomiasis</span> Medical condition

Trypanosomiasis or trypanosomosis is the name of several diseases in vertebrates caused by parasitic protozoan trypanosomes of the genus Trypanosoma. In humans this includes African trypanosomiasis and Chagas disease. A number of other diseases occur in other animals.

<i>Trypanosoma</i> Genus of parasitic flagellate protist in the Kinetoplastea class

Trypanosoma is a genus of kinetoplastids, a monophyletic group of unicellular parasitic flagellate protozoa. Trypanosoma is part of the phylum Sarcomastigophora. The name is derived from the Greek trypano- (borer) and soma (body) because of their corkscrew-like motion. Most trypanosomes are heteroxenous and most are transmitted via a vector. The majority of species are transmitted by blood-feeding invertebrates, but there are different mechanisms among the varying species. Some, such as Trypanosoma equiperdum, are spread by direct contact. In an invertebrate host they are generally found in the intestine, but normally occupy the bloodstream or an intracellular environment in the vertebrate host.

<i>Trypanosoma brucei</i> Species of protozoan parasite

Trypanosoma brucei is a species of parasitic kinetoplastid belonging to the genus Trypanosoma that is present in sub-Saharan Africa. Unlike other protozoan parasites that normally infect blood and tissue cells, it is exclusively extracellular and inhabits the blood plasma and body fluids. It causes deadly vector-borne diseases: African trypanosomiasis or sleeping sickness in humans, and animal trypanosomiasis or nagana in cattle and horses. It is a species complex grouped into three subspecies: T. b. brucei, T. b. gambiense and T. b. rhodesiense. The first is a parasite of non-human mammals and causes nagana, while the latter two are zoonotic infecting both humans and animals and cause African trypanosomiasis.

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<span class="mw-page-title-main">Animal trypanosomiasis</span> Parasitic disease of vertebrates

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Wigglesworthia glossinidia is a species of gram-negative bacteria which was isolated from the gut of the tsetse fly. W. glossinidia is a bacterial endosymbiont of the tsetse fly. Because of this relationship, Wigglesworthia has lost a large part of its genome and has one of the smallest known genomes of any living organism, consisting of a single chromosome of 700,000 bp and a plasmid of 5,200. Together with Buchnera aphidicola, Wigglesworthia has been the subject of genetic research into the minimal genome necessary for any living organism. Wigglesworthia also synthesizes key B-complex vitamins which the tsetse fly does not get from its diet of blood. Without the vitamins Wigglesworthia produces, the tsetse fly has greatly reduced growth and reproduction. Since the tsetse fly is the primary vector of Trypanosoma brucei, the pathogen that causes African trypanosomiasis, it has been suggested that W. glossinidia may one day be used to help control the spread of this disease.

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Fatma Serap Aksoy is a Turkish–American medical entomologist.

References

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  6. ↑ Pollock, J. N. (1982). Training Manual for Tsetse Control Personnel: Tsetse biology, systematics and distribution; techniques. FAO.
  7. ↑ Solano, Philippe; Salou, Ernest; Rayaisse, Jean-Baptiste; Ravel, Sophie; Gimonneau, Geoffrey; Traore, Ibrahima; Bouyer, Jérémy (2015-12-01). "Do tsetse flies only feed on blood?". Infection, Genetics and Evolution. 36: 184–189. doi: 10.1016/j.meegid.2015.09.016 . ISSN   1567-1348.
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  11. ↑ Chen, Xiaoai; Li, Song; Aksoy, Serap (1999-01-01). "Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia". Journal of Molecular Evolution . 48 (1): 49–58. Bibcode:1999JMolE..48...49C. doi:10.1007/PL00006444. ISSN   1432-1432. PMID   9873076. S2CID   30845386.
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  15. ↑
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