L-gulonate 3-dehydrogenase

L-gulonate 3-dehydrogenase
Identifiers
EC no.	1.1.1.45
CAS no.	9028-51-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a L-gulonate 3-dehydrogenase (EC 1.1.1.45) is an enzyme that catalyzes the chemical reaction

L-gulonic acid

+ NAD⁺

 

 

 

H⁺

 

H⁺

 

3-dehydro-L-gulonic acid

+ NADH

 

The two substrates of this enzyme are L-gulonic acid and oxidised nicotinamide adenine dinucleotide (NAD⁺). Its products are 3-dehydro-L-gulonic acid, reduced NADH, and a proton. ^{[1] [2] [3]}

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is L-gulonate:NAD⁺ 3-oxidoreductase. Other names in common use include L-3-aldonate dehydrogenase, L-3-aldonic dehydrogenase, L-gulonic acid dehydrogenase, L-beta-hydroxyacid dehydrogenase, L-beta-hydroxy-acid-NAD⁺-oxidoreductase, and L-3-hydroxyacid dehydrogenase. This enzyme participates in pentose and glucuronate interconversions.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 2DPO.

References

1. Enzyme 1.1.1.45 at KEGG Pathway Database.
2. Dworsky P, Hoffmann-Ostenhof O (1964). "L-3-Aldonic acid dehydrogenase from Schwanniomyces occidentalis". Acta Biochim. Pol. 11: 269–277.
3. Smiley JD, Ashwell G (1961). "Purification and properties of beta-L-hydroxy acid dehydrogenase II. Isolation of beta-keto-L-gluconic acid, an intermediate in L-xylulose biosynthesis". J. Biol. Chem. 236: 357–364. doi: 10.1016/S0021-9258(18)64367-4 .