Pentachlorophenol monooxygenase

Pentachlorophenol monooxygenase
Identifiers
EC no.	1.14.13.50
CAS no.	136111-57-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Pentachlorophenol monooxygenase (EC 1.14.13.50, pentachlorophenol dechlorinase, pentachlorophenol dehalogenase, pentachlorophenol 4-monooxygenase, PCP hydroxylase, pentachlorophenol hydroxylase, PcpB, PCB 4-monooxygenase, PCB4MO) is an enzyme with systematic name pentachlorophenol,NADPH:oxygen oxidoreductase (hydroxylating, dechlorinating). ^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

(1) pentachlorophenol + 2 NADPH + H⁺ + O2 ${\displaystyle \rightleftharpoons }$ 2,3,5,6-tetrachlorohydroquinone + 2 NADP⁺ + chloride + H₂O

(2) 2,3,5,6-tetrachlorophenol + NADPH + H⁺ + O2 ${\displaystyle \rightleftharpoons }$ 2,3,5,6-tetrachlorohydroquinone + NADP⁺ + H₂O

Pentachlorophenol monooxygenase is a flavoprotein (FAD).

References

1. Schenk T, Müller R, Mörsberger F, Otto MK, Lingens F (October 1989). "Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790". Journal of Bacteriology. 171 (10): 5487–91. doi:10.1128/jb.171.10.5487-5491.1989. PMC   210388 . PMID   2793827.
2. Xun L, Topp E, Orser CS (May 1992). "Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries". Journal of Bacteriology. 174 (9): 2898–902. doi:10.1128/jb.174.9.2898-2902.1992. PMC   205942 . PMID   1569020.
3. Xun L, Topp E, Orser CS (September 1992). "Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase". Journal of Bacteriology. 174 (17): 5745–7. doi:10.1128/jb.174.17.5745-5747.1992. PMC   206524 . PMID   1512208.
4. Lange CC, Schneider BJ, Orser CS (February 1996). "Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723". Biochemical and Biophysical Research Communications. 219 (1): 146–9. doi:10.1006/bbrc.1996.0196. PMID   8619798.
5. Nakamura T, Motoyama T, Hirono S, Yamaguchi I (August 2004). "Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase". Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 1700 (2): 151–9. doi:10.1016/j.bbapap.2004.04.008. PMID   15262224.

External links

• Pentachlorophenol+monooxygenase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)