Pentalenene synthase

pentalenene synthase
Identifiers
EC no.	4.2.3.7
CAS no.	90597-46-9
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

The enzyme pentalenene synthase (EC 4.2.3.7) catalyzes the chemical reaction

(2E,6E)-farnesyl diphosphate ${\displaystyle \rightleftharpoons }$ pentalenene + diphosphate

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is (2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, pentalenene-forming). This enzyme is also called pentalenene synthetase. The crystal structure of pentalenene synthase was the first of a bacterial terpene cyclase and was reported by the research group of David W. Christianson at the University of Pennsylvania. ^[1]

References

1. Lesburg, Charles A.; Zhai, Guangzhi; Cane, David E.; Christianson, David W. (1997). "Crystal Structure of Pentalenene Synthase: Mechanistic Insights on Terpenoid Cyclization Reactions in Biology". Science. 277 (5333): 1820–1824. doi:10.1126/science.277.5333.1820. PMID   9295272.

• Cane DE (1983). "Cell-free studies of monoterpene and sesquiterpene biosynthesis". Biochem. Soc. Trans. 11 (5): 510–5. doi:10.1042/bst0110510. PMID   6642060.
• Cane DE, Tillman AM (1983). "Pentalenene biosynthesis and the enzymic cyclization of farnesyl pyrophosphate". J. Am. Chem. Soc. 105 (1): 122–124. Bibcode:1983JAChS.105..122C. doi:10.1021/ja00339a026.
• Cane, DE, Hubbard BR; Sohng, JK; Lamberson, CR; Rudnicki, SM; Wu, Z; Lloyd, MD; Oliver, JS; Hubbard, BR (1994). "Pentalenene synthase. Purification, molecular cloning, sequencing, and high-level expression in Escherichia coli of a terpenoid cyclase from Streptomyces UC5319". Biochemistry. 33 (19): 5846–57. doi:10.1021/bi00185a024. PMID   8180213.