RAD23A

RAD23A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1DV0, 1F4I, 1IFY, 1OQY, 1P98, 1P9D, 1QZE, 1TP4, 2WYQ
Identifiers
Aliases	RAD23A , HHR23A, HR23A, RAD23 homolog A, nucleotide excision repair protein
External IDs	OMIM: 600061; MGI: 105126; HomoloGene: 48322; GeneCards: RAD23A; OMA:RAD23A - orthologs
Gene location (Human) Chr. Chromosome 19 (human) [1] Band 19p13.13 Start 12,945,855 bp [1] End 12,953,642 bp [1]
Gene location (Mouse) Chr. Chromosome 8 (mouse) [2] Band 8|8 C3 Start 85,560,648 bp [2] End 85,567,294 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in muscle of thigh gastrocnemius muscle Skeletal muscle tissue of rectus abdominis glutes triceps brachii muscle Skeletal muscle tissue of biceps brachii thoracic diaphragm body of tongue trabecular bone olfactory bulb Top expressed in muscle of thigh yolk sac lacrimal gland spermatocyte lip ankle temporal muscle triceps brachii muscle human fetus blood More reference expression data BioGPS More reference expression data
Gene ontology Molecular function single-stranded DNA binding ubiquitin-specific protease binding damaged DNA binding polyubiquitin modification-dependent protein binding protein binding kinase binding ubiquitin binding proteasome binding Cellular component proteasome complex nucleoplasm microtubule organizing center cytosol nucleus cytoplasm intracellular membrane-bounded organelle protein-containing complex Biological process nucleotide-excision repair positive regulation of viral genome replication viral process DNA repair cellular response to DNA damage stimulus proteasome-mediated ubiquitin-dependent protein catabolic process regulation of proteasomal ubiquitin-dependent protein catabolic process protein deubiquitination protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process positive regulation of cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5886 19358 Ensembl ENSG00000179262 ENSMUSG00000003813 UniProt P54725 P54726 RefSeq (mRNA) NM_001270362 NM_001270363 NM_005053 NM_001297606 NM_001297607 NM_009010 NM_001378894 NM_001378895 NM_001378896 RefSeq (protein) NP_001257291 NP_001257292 NP_005044 NP_001284535 NP_001284536 NP_033036 NP_001365823 NP_001365824 NP_001365825 Location (UCSC) Chr 19: 12.95 – 12.95 Mb Chr 8: 85.56 – 85.57 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

UV excision repair protein RAD23 homolog A is a protein that in humans is encoded by the RAD23A gene. ^[5]

Function

The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. ^[6]

RAD23A interacts with Y-family DNA polymerase iota (ι), DNA polymerase eta (η), DNA polymerase kappa and Y family DNA polymerase REV1. ^[7] These polymerases have roles in the DNA damage tolerance pathway of translesion synthesis that allows the replication fork to continue without stalling even when damage is present. ^[7]

Interactions

RAD23A has been shown to interact with:

• Ataxin 3, ^[8]
• PSMD4, ^{[9] [10]} and
• Sequestosome 1. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000179262 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000003813 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. van der Spek PJ, Smit EM, Beverloo HB, Sugasawa K, Masutani C, Hanaoka F, Hoeijmakers JH, Hagemeijer A (March 1995). "Chromosomal localization of three repair genes: the xeroderma pigmentosum group C gene and two human homologs of yeast RAD23". Genomics. 23 (3): 651–658. doi:10.1006/geno.1994.1554. hdl: 1765/3069 . PMID   7851894.
6. "Entrez Gene: RAD23A RAD23 homolog A (S. cerevisiae)".
7. Ashton NW, Jaiswal N, Moreno NC, Semenova IV, D'Orlando DA, Latancia MT, McIntyre J, Woodgate R, Bezsonova I (December 2023). "A Novel Interaction Between RAD23A/B and Y-family DNA Polymerases". J Mol Biol. 435 (24): 168353. doi:10.1016/j.jmb.2023.168353. PMID   37935254.
8. Wang G, Sawai N, Kotliarova S, Kanazawa I, Nukina N (July 2000). "Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B". Hum. Mol. Genet. 9 (12): 1795–803. doi: 10.1093/hmg/9.12.1795 . PMID   10915768.
9. Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F (September 1999). "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome". J. Biol. Chem. 274 (39): 28019–25. doi: 10.1074/jbc.274.39.28019 . hdl: 1765/9166 . PMID   10488153.
10. Mueller TD, Feigon J (September 2003). "Structural determinants for the binding of ubiquitin-like domains to the proteasome". EMBO J. 22 (18): 4634–45. doi:10.1093/emboj/cdg467. PMC   212733 . PMID   12970176.
11. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.

Further reading

• Eichbaum QG, Beatty DW, Parker MI (1994). "Identification of cardiac autoantigens in human heart cDNA libraries using acute rheumatic fever sera". J. Autoimmun. 7 (2): 243–261. doi:10.1006/jaut.1994.1019. PMID   8037842.
• Masutani C, Sugasawa K, Yanagisawa J, Sonoyama T, Ui M, Enomoto T, Takio K, Tanaka K, van der Spek PJ, Bootsma D (1994). "Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homologue of yeast RAD23". EMBO J. 13 (8): 1831–43. doi:10.1002/j.1460-2075.1994.tb06452.x. PMC   395023 . PMID   8168482.
• van der Spek PJ, Eker A, Rademakers S, Visser C, Sugasawa K, Masutani C, Hanaoka F, Bootsma D, Hoeijmakers JH (1996). "XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes". Nucleic Acids Res. 24 (13): 2551–2559. doi:10.1093/nar/24.13.2551. PMC   145966 . PMID   8692695.
• van der Spek PJ, Visser CE, Hanaoka F, Smit B, Hagemeijer A, Bootsma D, Hoeijmakers JH (1996). "Cloning, comparative mapping, and RNA expression of the mouse homologues of the Saccharomyces cerevisiae nucleotide excision repair gene RAD23". Genomics. 31 (1): 20–27. doi:10.1006/geno.1996.0004. hdl: 1765/3097 . PMID   8808275.
• Li L, Lu X, Peterson C, Legerski R (1997). "XPC interacts with both HHR23B and HHR23A in vivo". Mutat. Res. 383 (3): 197–203. doi:10.1016/s0921-8777(97)00002-5. PMID   9164480.
• Withers-Ward ES, Jowett JB, Stewart SA, Xie YM, Garfinkel A, Shibagaki Y, Chow SA, Shah N, Hanaoka F, Sawitz DG, Armstrong RW, Souza LM, Chen IS (1997). "Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair". J. Virol. 71 (12): 9732–42. doi:10.1128/JVI.71.12.9732-9742.1997. PMC   230283 . PMID   9371639.
• Schauber C, Chen L, Tongaonkar P, Vega I, Lambertson D, Potts W, Madura K (1998). "Rad23 links DNA repair to the ubiquitin/proteasome pathway". Nature. 391 (6668): 715–718. Bibcode:1998Natur.391..715S. doi:10.1038/35661. PMID   9490418. S2CID   4417020.
• Gragerov A, Kino T, Ilyina-Gragerova G, Chrousos GP, Pavlakis GN (1998). "HHR23A, the human homologue of the yeast repair protein RAD23, interacts specifically with Vpr protein and prevents cell cycle arrest but not the transcriptional effects of Vpr". Virology. 245 (2): 323–330. doi: 10.1006/viro.1998.9138 . PMID   9636371.
• Dieckmann T, Withers-Ward ES, Jarosinski MA, Liu CF, Chen IS, Feigon J (1998). "Structure of a human DNA repair protein UBA domain that interacts with HIV-1 Vpr". Nat. Struct. Biol. 5 (12): 1042–1047. doi:10.1038/4220. PMID   9846873. S2CID   30478711.
• Kumar S, Talis AL, Howley PM (1999). "Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination". J. Biol. Chem. 274 (26): 18785–18792. doi: 10.1074/jbc.274.26.18785 . PMID   10373495.
• Hiyama H, Yokoi M, Masutani C, Sugasawa K, Maekawa T, Tanaka K, Hoeijmakers JH, Hanaoka F (1999). "Interaction of hHR23 with S5a. The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26 S proteasome". J. Biol. Chem. 274 (39): 28019–28025. doi: 10.1074/jbc.274.39.28019 . hdl: 1765/9166 . PMID   10488153.
• Miao F, Bouziane M, Dammann R, Masutani C, Hanaoka F, Pfeifer G, O'Connor TR (2000). "3-Methyladenine-DNA glycosylase (MPG protein) interacts with human RAD23 proteins". J. Biol. Chem. 275 (37): 28433–28438. doi: 10.1074/jbc.M001064200 . PMID   10854423.
• Wang G, Sawai N, Kotliarova S, Kanazawa I, Nukina N (2000). "Ataxin-3, the MJD1 gene product, interacts with the two human homologs of yeast DNA repair protein RAD23, HHR23A and HHR23B". Hum. Mol. Genet. 9 (12): 1795–1803. doi: 10.1093/hmg/9.12.1795 . PMID   10915768.
• Withers-Ward ES, Mueller TD, Chen IS, Feigon J (2001). "Biochemical and structural analysis of the interaction between the UBA(2) domain of the DNA repair protein HHR23A and HIV-1 Vpr". Biochemistry. 39 (46): 14103–14112. doi:10.1021/bi0017071. PMID   11087358.
• Zhu Q, Wani G, Wani MA, Wani AA (2001). "Human homologue of yeast Rad23 protein A interacts with p300/cyclic AMP-responsive element binding (CREB)-binding protein to down-regulate transcriptional activity of p53". Cancer Res. 61 (1): 64–70. PMID   11196199.
• Mansky LM, Preveral S, Le Rouzic E, Bernard LC, Selig L, Depienne C, Benarous R, Benichou S (2001). "Interaction of human immunodeficiency virus type 1 Vpr with the HHR23A DNA repair protein does not correlate with multiple biological functions of Vpr". Virology. 282 (1): 176–185. doi: 10.1006/viro.2000.0791 . PMID   11259200.
• Gaynor EM, Chen IS (2001). "Analysis of apoptosis induced by HIV-1 Vpr and examination of the possible role of the hHR23A protein". Exp. Cell Res. 267 (2): 243–257. doi:10.1006/excr.2001.5247. PMID   11426943.
• Chen L, Shinde U, Ortolan TG, Madura K (2002). "Ubiquitin-associated (UBA) domains in Rad23 bind ubiquitin and promote inhibition of multi-ubiquitin chain assembly". EMBO Rep. 2 (10): 933–938. doi:10.1093/embo-reports/kve203. PMC   1084081 . PMID   11571271.
• Elder RT, Song XQ, Chen M, Hopkins KM, Lieberman HB, Zhao Y (2002). "Involvement of rhp23, a Schizosaccharomyces pombe homolog of the human HHR23A and Saccharomyces cerevisiae RAD23 nucleotide excision repair genes, in cell cycle control and protein ubiquitination". Nucleic Acids Res. 30 (2): 581–591. doi:10.1093/nar/30.2.581. PMC   99819 . PMID   11788722.