Vanillyl-alcohol oxidase

vanillyl-alcohol oxidase
Vanillyl-alcohol oxidase homooctamer, Penicillium simplicissimum
Identifiers
EC no.	1.1.3.38
CAS no.	143929-24-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a vanillyl-alcohol oxidase (EC 1.1.3.38) is an enzyme that catalyzes the chemical reaction

+ O₂${\displaystyle \rightleftharpoons }$ + H₂O₂

Thus, the two substrates of this enzyme are vanillyl alcohol and O₂, whereas its two products are vanillin and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is vanillyl alcohol:oxygen oxidoreductase. This enzyme is also called 4-hydroxy-2-methoxybenzyl alcohol oxidase. This enzyme participates in 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1DZN, 1E0Y, and 1E8G.

References

• de Jong E, van Berkel WJ, van der Zwan RP, de Bont JA (1992). "Purification and characterization of vanillyl-alcohol oxidase from Penicillium simplicissimum. A novel aromatic alcohol oxidase containing covalently bound FAD". Eur. J. Biochem. 208 (3): 651–657. doi: 10.1111/j.1432-1033.1992.tb17231.x . PMID   1396672.
• Fraaije MW, Veeger C, van Berkel WJ (1995). "Substrate specificity of flavin-dependent vanillyl-alcohol oxidase from Penicillium simplicissimum. Evidence for the production of 4-hydroxycinnamyl alcohols from 4-allylphenols" (PDF). Eur. J. Biochem. 234 (1): 271–277. doi: 10.1111/j.1432-1033.1995.271_c.x . PMID   8529652.