Cholesterol oxidase

Last updated
cholesterol oxidase
Identifiers
EC no. 1.1.3.6
CAS no. 9028-76-6
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO
Search
PMC articles
PubMed articles
NCBI proteins
Cholesterol oxidase substrate-binding domain
PDB 1i19 EBI.jpg
crystal structure of cholesterol oxidase from b.sterolicum
Identifiers
SymbolChol_subst-bind
Pfam PF09129
Pfam clan CL0277
InterPro IPR015213
SCOP2 1i19 / SCOPe / SUPFAM
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

In enzymology, a cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the chemical reaction

Contents

cholesterol + O2 cholest-4-en-3-one + H2O2

Thus, the two substrates of this enzyme are cholesterol and O2, whereas its two products are cholest-4-en-3-one and H2O2.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

The substrate-binding domain found in some bacterial cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. This domain is positioned over the isoalloxazine ring system of the FAD cofactor bound by the FAD-binding domain and forms the roof of the active site cavity, allowing for catalysis of oxidation and isomerisation of cholesterol to cholest-4-en-3-one. [1]

Structural studies

As of late 2007, 14 structures have been solved for this class of enzymes, with PDB accession codes 1B4V, 1B8S, 1CBO, 1CC2, 1COY, 1I19, 1IJH, 1MXT, 1N1P, 1N4U, 1N4V, 1N4W, 2GEW, and 3COX.

Related Research Articles

In enzymology, a sterol-4alpha-carboxylate 3-dehydrogenase (decarboxylating) (EC 1.1.1.170) is an enzyme that catalyzes the chemical reaction

In enzymology, a 3-keto-steroid reductase (EC 1.1.1.270) is an enzyme that catalyzes the chemical reaction

In enzymology, a tryptophan alpha,beta-oxidase (EC 1.3.3.10) is an enzyme that catalyzes the chemical reaction

In enzymology, a 24-hydroxycholesterol 7alpha-hydroxylase (EC 1.14.13.99) is an enzyme that catalyzes the chemical reaction

In enzymology, a Δ7-sterol 5(6)-desaturase is an enzyme that catalyzes the chemical reaction

<span class="mw-page-title-main">Choline oxidase</span>

In enzymology, a choline oxidase (EC 1.1.3.17) is an enzyme that catalyzes the chemical reaction

In enzymology, a hexose oxidase (EC 1.1.3.5) is an enzyme that catalyzes the chemical reaction

In enzymology, a pyridoxine 4-oxidase (EC 1.1.3.12) is an enzyme that catalyzes the chemical reaction

In enzymology, a thiamine oxidase (EC 1.1.3.23) is an enzyme that catalyzes the chemical reaction

A pyruvate oxidase is basically an enzyme that speeds up a chemical reaction.

<span class="mw-page-title-main">NADH peroxidase</span>

In enzymology, a NADH peroxidase (EC 1.11.1.1) is an enzyme that catalyzes the chemical reaction

In enzymology, a D-aspartate oxidase (EC 1.4.3.1) is an enzyme that catalyzes the chemical reaction

In enzymology, a dimethylglycine oxidase (EC 1.5.3.10) is an enzyme that catalyzes the chemical reaction

In enzymology, an ethanolamine oxidase (EC 1.4.3.8) is an enzyme that catalyzes the chemical reaction

In enzymology, a glutathione oxidase (EC 1.8.3.3) is an enzyme that catalyzes the chemical reaction

In enzymology, a L-aspartate oxidase (EC 1.4.3.16) is an enzyme that catalyzes the chemical reaction

In enzymology, a L-glutamate oxidase (EC 1.4.3.11) is an enzyme that catalyzes the chemical reaction

In enzymology, a nitroalkane oxidase (EC 1.7.3.1) is an enzyme that catalyzes the chemical reaction

In enzymology, a prenylcysteine oxidase (EC 1.8.3.5) is an enzyme that catalyzes the chemical reaction

Methylsterol monooxygenase (EC 1.14.13.72, methylsterol hydroxylase, 4-methylsterol oxidase, 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,hydrogen-donor:oxygen oxidoreductase (hydroxylating)) is an enzyme with systematic name 4,4-dimethyl-5alpha-cholest-7-en-3beta-ol,NAD(P)H:oxygen oxidoreductase (hydroxylating). This enzyme catalyses the following chemical reaction

References

  1. Coulombe R, Yue KQ, Ghisla S, Vrielink A (August 2001). "Oxygen access to the active site of cholesterol oxidase through a narrow channel is gated by an Arg-Glu pair". J. Biol. Chem. 276 (32): 30435–41. doi: 10.1074/jbc.M104103200 . PMID   11397813.

Further reading

This article incorporates text from the public domain Pfam and InterPro: IPR015213