| cholesterol oxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.3.6 | ||||||||
| CAS no. | 9028-76-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
| Cholesterol oxidase substrate-binding domain | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 crystal structure of cholesterol oxidase from b.sterolicum | |||||||||
| Identifiers | |||||||||
| Symbol | Chol_subst-bind | ||||||||
| Pfam | PF09129 | ||||||||
| Pfam clan | CL0277 | ||||||||
| InterPro | IPR015213 | ||||||||
| SCOP2 | 1i19 / SCOPe / SUPFAM | ||||||||
| |||||||||
In enzymology, cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are cholesterol and oxygen. Its products are cholest-4-en-3-one (cholesterone) and hydrogen peroxide. [1] [2] [3]
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.
The substrate-binding domain found in some bacterial cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. This domain is positioned over the isoalloxazine ring system of the FAD cofactor bound by the FAD-binding domain and forms the roof of the active site cavity, allowing for catalysis of oxidation and isomerisation of cholesterol to cholest-4-en-3-one. [4]
