Clavaminate synthase

Clavaminate synthase
Identifiers
EC no.	1.14.11.21
CAS no.	122799-56-8
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Search PMC articles PubMed articles NCBI proteins

Clavaminate synthase (EC 1.14.11.21, clavaminate synthase 2, clavaminic acid synthase) is an enzyme with systematic name deoxyamidinoproclavaminate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating). ^{[1] [2] [3] [4] [5]} This enzyme catalyses the following chemical reaction

(1) deoxyamidinoproclavaminate + 2-oxoglutarate + O₂${\displaystyle \rightleftharpoons }$ amidinoproclavaminate + succinate + CO₂

(2) proclavaminate + 2-oxoglutarate + O₂${\displaystyle \rightleftharpoons }$ dihydroclavaminate + succinate + CO₂ + H₂O

(3) dihydroclavaminate + 2-oxoglutarate + O₂${\displaystyle \rightleftharpoons }$ clavaminate + succinate + CO₂ + H₂O

Clavaminate synthase contains nonheme iron.

References

1. Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA (February 1991). "Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction". Biochemistry. 30 (8): 2281–92. doi:10.1021/bi00222a034. PMID   1998687.
2. Zhou, J.; Gunsior, M.; Bachmann, B.O.; Townsend, C.A.; Solomon, E.I. (1998). "Substrate binding to the α-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation". J. Am. Chem. Soc. 120: 13539–13540. doi:10.1021/ja983534x.
3. Zhang Z, Ren J, Stammers DK, Baldwin JE, Harlos K, Schofield CJ (February 2000). "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase". Nature Structural Biology. 7 (2): 127–33. doi:10.1038/72398. PMID   10655615.
4. Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI (August 2001). "Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities". Journal of the American Chemical Society. 123 (30): 7388–98. doi:10.1021/ja004025+. PMID   11472170.
5. Townsend CA (October 2002). "New reactions in clavulanic acid biosynthesis". Current Opinion in Chemical Biology. 6 (5): 583–9. doi:10.1016/S1367-5931(02)00392-7. PMID   12413541.

External links

• Clavaminate+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)