Clavaminate synthase

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Clavaminate synthase
Identifiers
EC no. 1.14.11.21
CAS no. 122799-56-8
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Clavaminate synthase (EC 1.14.11.21, clavaminate synthase 2, clavaminic acid synthase) is an enzyme with systematic name deoxyamidinoproclavaminate,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating). [1] [2] [3] [4] [5] This enzyme catalyses the following chemical reaction

(1) deoxyamidinoproclavaminate + 2-oxoglutarate + O2 amidinoproclavaminate + succinate + CO2
(2) proclavaminate + 2-oxoglutarate + O2 dihydroclavaminate + succinate + CO2 + H2O
(3) dihydroclavaminate + 2-oxoglutarate + O2 clavaminate + succinate + CO2 + H2O

Clavaminate synthase contains nonheme iron.

References

  1. ↑ Salowe SP, Krol WJ, Iwata-Reuyl D, Townsend CA (February 1991). "Elucidation of the order of oxidations and identification of an intermediate in the multistep clavaminate synthase reaction". Biochemistry. 30 (8): 2281–92. doi:10.1021/bi00222a034. PMID   1998687.
  2. ↑ Zhou, J.; Gunsior, M.; Bachmann, B.O.; Townsend, C.A.; Solomon, E.I. (1998). "Substrate binding to the α-ketoglutarate-dependent non-heme iron enzyme clavaminate synthase 2: Coupling mechanism of oxidative decarboxylation and hydroxylation". J. Am. Chem. Soc. 120 (51): 13539–13540. Bibcode:1998JAChS.12013539Z. doi:10.1021/ja983534x.
  3. ↑ Zhang Z, Ren J, Stammers DK, Baldwin JE, Harlos K, Schofield CJ (February 2000). "Structural origins of the selectivity of the trifunctional oxygenase clavaminic acid synthase". Nature Structural Biology. 7 (2): 127–33. doi:10.1038/72398. PMID   10655615.
  4. ↑ Zhou J, Kelly WL, Bachmann BO, Gunsior M, Townsend CA, Solomon EI (August 2001). "Spectroscopic studies of substrate interactions with clavaminate synthase 2, a multifunctional alpha-KG-dependent non-heme iron enzyme: correlation with mechanisms and reactivities". Journal of the American Chemical Society. 123 (30): 7388–98. Bibcode:2001JAChS.123.7388Z. doi:10.1021/ja004025+. PMID   11472170.
  5. ↑ Townsend CA (October 2002). "New reactions in clavulanic acid biosynthesis". Current Opinion in Chemical Biology. 6 (5): 583–9. doi:10.1016/S1367-5931(02)00392-7. PMID   12413541.
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