| Name | Mark | System [nb 1] | From | To | Successor | Notes |
|---|
| Alexander and Eastern Railway | | | 1906 | | N/A |
| Alexander and Rich Mountain Railroad | | | 1892 | 1899 | Alexander and Rich Mountain Railway |
| Alexander and Rich Mountain Railway | | | 1899 | 1906 | Alexander and Eastern Railway |
| Allegheny and Western Railway | | NYC | 1905 | 1918 | Kanawha and West Virginia Railroad |
| Atlantic and Northwestern Railroad | | NYC | 1881 | 1882 | Ohio Central Railroad |
| Baltimore and Ohio Railroad | B&O, BO | B&O | 1827 | 1987 | Chesapeake and Ohio Railway |
| Belington and Beaver Creek Railroad | | WM | 1899 | 1905 | Western Maryland Railroad |
| Belington and Northern Railroad | | B&O | 1900 | 1941 | N/A |
| Benwood and Wheeling Connecting Railway | | | 1900 | 1960 | N/A |
| Berkeley Springs Railroad | | B&O | 1911 | 1912 | Baltimore and Ohio Railroad |
| Berkeley Springs and Potomac Railroad | | B&O | 1880 | 1910 | Berkeley Springs Railroad |
| Big Sandy and Cumberland Railroad | BS&C | N&W | 1900 | 1932 | Norfolk and Western Railway |
| Big Sandy, East Lynn and Guyan Railroad | | N&W | 1902 | 1908 | Norfolk and Western Railway |
| Big Stony Railway | | N&W | 1910 | 1915 | Norfolk and Western Railway |
| Black Fork Railroad | | | 1912 | 1913 | Central West Virginia and Southern Railroad |
| Blacksville and Western Railway | | MGA | 1917 | 1917 | Morgantown and Wheeling Railway |
| Blackwater and Potomac Railroad | | | 1915 | 1922 | N/A |
| Blue Stone Railroad | | N&W | 1881 | 1881 | New River Railroad of West Virginia |
| Buckhannon and Northern Railroad | | MGA | 1902 | 1915 | Monongahela Railway |
| Buckhannon River Railroad | | B&O | 1889 | 1890 | West Virginia and Pittsburg Railroad |
| Buckhannon River Lumber Company | | B&O | 1885 | 1889 | Clarksburg, Weston and Midland Railroad |
| Buckhannon and West Fork Railroad | | B&O | 1882 | 1890 | Clarksburg, Weston and Midland Railroad |
| Buffalo Creek and Gauley Railroad | | | 1904 | 1965 | Elk River Railroad |
| Cairo and Kanawha Railway | | | 1906 | | N/A |
| Cairo and Kanawha Valley Railroad | | | 1890 | 1906 | Cairo and Kanawha Railway |
| Campbell's Creek Railroad | CCK | | 1901 | 1962 | N/A |
| Caretta Railway | | N&W | 1906 | 1909 | Norfolk and Western Railway |
| Central Railway of West Virginia | | B&O | 1898 | 1902 | Morgantown and Kingwood Railroad |
| Central West Virginia and Southern Railroad | | | 1913 | 1936 | N/A |
| Chaffee Railroad | | WM | 1918 | 1950 | Western Maryland Railway |
| Charleston, Clendenin and Sutton Railroad | | B&O | 1891 | 1903 | Coal and Coke Railway |
| Charleston and Gauley Railway | | NYC | 1887 | 1890 | Kanawha and Michigan Railway |
| Cheat Haven and Bruceton Railroad | | B&O | 1911 | 1965 | N/A |
| Cheat River and Pittsburg Railroad | | B&O | | 1902 | Morgantown and Kingwood Railroad |
| Cheat Valley Railroad | | B&O | 1897 | 1904 | Morgantown and Kingwood Railroad |
| Chemical and Helvetia Railroad | | | 1909 | | N/A |
| Cherry Run and Potomac Valley Railroad | | B&O | 1892 | 1912 | Baltimore and Ohio Railroad |
| Chesapeake and Ohio Railroad | | C&O | 1868 | 1878 | Chesapeake and Ohio Railway |
| Chesapeake and Ohio Railway | C&O, CO | C&O | 1878 | 1987 | CSX Transportation |
| Clarksburg, Weston and Glenville Railroad and Transportation Company | | B&O | 1878 | 1889 | Clarksburg, Weston and Midland Railroad |
| Clarksburg, Weston and Midland Railroad | | B&O | 1890 | 1890 | West Virginia and Pittsburg Railroad |
| Clendenin and Spencer Railroad | | | | 1904 | Kanawha and Northern Railway |
| Coal and Coke Railway | C&C | B&O | 1902 | 1933 | Baltimore and Ohio Railroad |
| Coal and Iron Railway | | WM | 1899 | 1905 | Western Maryland Railroad |
| Coal River Railway | | C&O | 1905 | 1909 | Chesapeake and Ohio Railway |
| Coal River and Western Railway | | C&O | 1903 | 1905 | Coal River Railway |
| Consolidated Rail Corporation | CR | | 1976 | 1999 | Norfolk Southern Railway |
| Covington and Ohio Railroad | | C&O | 1853 | 1868 | Chesapeake and Ohio Railroad |
| Croft Railroad | | | 1910 | | N/A |
| Cross Creek Railroad | | P&WV | 1900 | 1904 | Wabash Pittsburgh Terminal Railway |
| Cumberland and Pennsylvania Railroad | C&PA, CPA | WM | 1863 | 1953 | Western Maryland Railway |
| Cumberland Valley Railroad | | PRR | 1873 | 1919 | N/A | Leased the Cumberland Valley and Martinsburg Railroad |
| Cumberland Valley and Martinsburg Railroad | | PRR | 1888 | 1958 | Penndel Company |
| Deepwater Railway | | N&W | 1898 | 1907 | Virginian Railway |
| Dry Fork Railroad | | | 1892 | 1913 | Central West Virginia and Southern Railroad |
| Elk and Little Kanawha Railroad | | | 1909 | 1919 | N/A |
| Elm Grove and State Line Railroad | | PRR | 1883 | 1884 | Wheeling and Harrisburg Railway |
| Erbacon and Summersville Railroad | | | 1911 | | N/A |
| Everson and Adamsville Railroad | | B&O | 1902 | 1903 | Monongahela River Railroad |
| Fairmont Bingamon Railway | | WM | 1916 | 1950 | Western Maryland Railway |
| Fairmont Helen's Run Railway | | WM | 1915 | 1950 | Western Maryland Railway |
| Fairmont, Morgantown and Pittsburg Railroad | | B&O | 1883 | 1989 | CSX Transportation |
| Fairmont, Shinnston and Clarksburg Railway | | B&O | 1884 | 1912 | Baltimore and Ohio Railroad |
| Gaston Gas Coal Company | | B&O | 1883 | 1889 | Monongahela River Railroad |
| Gauley and Eastern Railway | | NYC | 1889 | 1920 | Kanawha and West Virginia Railroad |
| Gauley and Meadow River Railroad | | C&O | 1912 | 1918 | Chesapeake and Ohio Railway |
| Gauley River Railroad | GRIV | | 1998 | 1999 | N/A | Never operated |
| Glade Creek and Raleigh Railroad | | C&O | 1891 | 1906 | Raleigh and Southwestern Railway |
| Glady and Alpena Railroad | | | 1901 | 1922 | N/A |
| Glen Jean, Lower Loup and Deepwater Railroad | | C&O | 1895 | 1901 | Chesapeake and Ohio Railway |
| Grafton and Belington Railroad | | B&O | 1892 | 1912 | Baltimore and Ohio Railroad |
| Grafton and Greenbrier Railroad | | B&O | 1881 | 1892 | Grafton and Belington Railroad |
| Greenbrier Railway | | C&O | 1897 | 1903 | Chesapeake and Ohio Railway |
| Greenbrier, Cheat and Elk Railroad | | WM | 1910 | 1927 | Western Maryland Railway |
| Greenbrier and Eastern Railroad | | C&O/ NYC | 1919 | 1931 | Nicholas, Fayette and Greenbrier Railroad |
| Greenbrier and New River Railroad | | C&O | 1881 | 1901 | Chesapeake and Ohio Railway |
| Guyan, Big Ugly and Coal River Railway | | | 1907 | 1921 | N/A |
| Guyandotte Valley Railroad Company | | C&O | 1899 | 1903 | Chesapeake and Ohio Railway |
| Guyandotte and Ohio River Railroad and Mineral Company | | NYC | 1872 | 1881 | Atlantic and Northwestern Railroad |
| Hampshire Southern Railroad | | B&O | 1906 | 1911 | Moorefield and Virginia Railroad |
| Harrisville and Cornwallis Railroad | | | 1908 | 1911 | Harrisville Southern Railroad |
| Harrisville Southern Railroad | | | 1911 | | N/A |
| Hartland Railroad | | | 1922 | 1925 | Middle Creek Railroad |
| Hempfield Railroad | | B&O | 1851 | 1871 | Wheeling, Pittsburgh and Baltimore Railroad |
| Holliday's Cove Railroad | | PRR | 1860 | 1868 | Pittsburgh, Cincinnati and St. Louis Railway |
| Holly River Railroad | | | 1896 | 1899 | Holly River and Addison Railway |
| Holly River and Addison Railway | | | 1898 | 1906 | West Virginia Midland Railroad |
| Huntington and Big Sandy Railroad | | B&O | 1890 | 1912 | Baltimore and Ohio Railroad |
| Iaeger and Southern Railway | | N&W | 1898 | 1906 | Norfolk and Western Railway |
| Imboden and Odell Railroad | | NYC | 1903 | 1905 | Kanawha and West Virginia Railroad |
| Indian Creek and Northern Railway | | MGA | 1918 | 1933 | Monongahela Railway |
| Interior and West Virginia Railroad | | N&W | 1906 | 1910 | Big Stony Railway |
| Iron Mountain and Greenbrier Railroad | | | | 1912 | White Sulphur and Huntersville Railroad |
| Iron Valley and Morgantown Railroad | | B&O | 1881 | 1883 | West Virginia Midland Railway |
| Island Creek Railroad | | C&O | 1902 | 1933 | Chesapeake and Ohio Railway |
| Kanawha Railway | | C&O | 1881 | 1902 | Chesapeake and Ohio Railway |
| Kanawha Bridge and Terminal Company | | C&O | 1907 | 1918 | Chesapeake and Ohio Railway |
| Kanawha Central Railway | KC | | 1906 | 1977 | N/A |
| Kanawha and Coal River Railroad | | | 1881 | | N/A |
| Kanawha, Glen Jean and Eastern Railroad | | C&O | 1895 | 1940 | Chesapeake and Ohio Railway |
| Kanawha and Michigan Railway | K&M | NYC | 1890 | 1938 | Toledo and Ohio Central Railway |
| Kanawha and Northern Railway | | | | 1906 | N/A |
| Kanawha and Ohio Railway | | NYC | 1886 | 1890 | Kanawha and Michigan Railway |
| Kanawha and Paint Creek Railroad | | C&O | 1884 | 1895 | Kanawha and Paint Creek Railway |
| Kanawha and Paint Creek Railway | | C&O | 1895 | 1902 | Kanawha and Pocahontas Railroad |
| Kanawha and Pennsylvania Railway | | NYC | 1903 | 1917 | Kanawha and West Virginia Railroad |
| Kanawha and Pocahontas Railroad | | C&O | 1898 | 1905 | Chesapeake and Ohio Railway |
| Kanawha and West Virginia Railroad | K&WV | NYC | 1905 | 1938 | Toledo and Ohio Central Railway |
| Kelly's Creek Railroad | | | 1916 | 1964 | N/A |
| Kelly's Creek and Northwestern Railroad | KCNW | | 1903 | 1993 | N/A |
| Kenova and Big Sandy Railroad | | N&W | 1902 | 1904 | Norfolk and Western Railway |
| Kingwood Railway | | | 1882 | 1888 | Tunnelton, Kingwood and Fairchance Railroad |
| Little Kanawha Railroad | | B&O | 1896 | 1937 | N/A |
| Logan and Southern Railway | | C&O | 1907 | 1918 | Chesapeake and Ohio Railway |
| Loop and Lookout Railroad | | C&O/ NYC | 1907 | 1931 | Nicholas, Fayette and Greenbrier Railroad |
| Lorama Railroad | | | 1903 | 1924 | N/A |
| Mann's Creek Railroad | | | 1886 | 1955 | N/A |
| Marlinton and Camden Railroad | | | 1903 | | N/A |
| Martinsburg and Potomac Railroad | | PRR | 1868 | 1888 | Cumberland Valley and Martinsburg Railroad |
| Middle Creek Railroad | | | 1925 | 1951 | N/A |
| Monongah and Ohio River Railroad | | B&O | 1895 | ca. 1900 | Monongahela River Railroad |
| Monongahela Railway | MGA | MGA | 1915 | 1993 | Consolidated Rail Corporation |
| Monongahela River Railroad | | B&O | 1888 | 1912 | Baltimore and Ohio Railroad |
| Moorefield and Virginia Railroad | | B&O | 1910 | 1912 | Baltimore and Ohio Railroad |
| Morgantown and Dunkard Valley Railroad | | MGA | 1905 | 1913 | Morgantown and Wheeling Railway |
| Morgantown and Kingwood Railroad | M&K | B&O | 1899 | | Baltimore and Ohio Railroad |
| Morgantown and Wheeling Railway | M&W | MGA | 1912 | 1923 | Scotts Run Railway |
| New River Railroad, Mining and Manufacturing Company of West Virginia | | N&W | 1874 | 1881 | New River Railroad of West Virginia |
| New River Railroad of West Virginia | | N&W | 1881 | 1882 | Norfolk and Western Railroad |
| New River Bridge Company | | C&O | 1887 | 1890 | Chesapeake and Ohio Railway |
| New York Central Railroad | NYC | NYC | 1922 | 1968 | Penn Central Transportation Company |
| New York, Chicago and St. Louis Railroad | NKP | NKP | 1949 | 1964 | Norfolk and Western Railway |
| Nicholas, Fayette and Greenbrier Railroad | | C&O/ NYC | 1926 | 1996 | Consolidated Rail Corporation, CSX Transportation |
| Norfolk and Western Railroad | | N&W | 1882 | 1896 | Norfolk and Western Railway |
| Norfolk and Western Railway | N&W, NW | N&W | 1896 | 1998 | Norfolk Southern Railway |
| North Western Virginia Railroad | | B&O | 1851 | 1865 | Parkersburg Branch Railroad |
| Ohio Central Railroad | | NYC | 1882 | 1885 | Kanawha and Ohio Railway |
| Ohio and Little Kanawha Railroad | | B&O | 1900 | 1910 | Baltimore and Ohio Railroad |
| Ohio River Railroad | | B&O | 1882 | 1912 | Baltimore and Ohio Railroad |
| Pan Handle Railroad | | PRR | 1868 | 1871 | Pittsburgh, Wheeling and Kentucky Railroad |
| Pan Handle Railway | | PRR | 1867 | 1868 | Pittsburgh, Cincinnati and St. Louis Railway |
| Panther Railroad | | | 1895 | | N/A |
| Parkersburg Branch Railroad | | B&O | 1865 | 1912 | Baltimore and Ohio Railroad |
| Patterson's Creek and Potomac Railroad | | B&O | 1900 | 1912 | Baltimore and Ohio Railroad |
| Paw Paw Railroad | | B&O | 1900 | 1912 | Baltimore and Ohio Railroad |
| Penn Central Transportation Company | PC | | 1968 | 1976 | Consolidated Rail Corporation |
| Penndel Company | | PRR | 1954 | 1976 | Consolidated Rail Corporation |
| Pennsboro and Harrisville Railroad | | | 1873 | 1879 | Pennsboro and Harrisville Ritchie County Railway |
| Pennsboro and Harrisville Ritchie County Railway | | | 1880 | 1905 | Lorama Railroad |
| Pennsylvania Railroad | PRR | PRR | 1920 | 1968 | Penn Central Transportation Company |
| Philadelphia, Baltimore and Washington Railroad | | PRR | 1956 | 1976 | Consolidated Rail Corporation |
| Pickens and Addison Railway | | | 1903 | | N/A |
| Pickens and Hackers Valley Railroad | | | 1899 | 1921 | N/A |
| Pickens and Webster Springs Railroad | | | 1912 | 1915 | N/A |
| Piedmont and Cumberland Railway | | WM | 1886 | 1905 | Western Maryland Railroad |
| Piney River and Paint Creek Railroad | | C&O | 1905 | 1918 | Chesapeake and Ohio Railway |
| Pittsburgh, Cincinnati, Chicago and St. Louis Railroad | | PRR | 1917 | 1956 | Philadelphia, Baltimore and Washington Railroad |
| Pittsburgh, Cincinnati, Chicago and St. Louis Railway | | PRR | 1890 | 1917 | Pittsburgh, Cincinnati, Chicago and St. Louis Railroad |
| Pittsburgh, Cincinnati and St. Louis Railway | | PRR | 1868 | 1890 | Pittsburgh, Cincinnati, Chicago and St. Louis Railway |
| Pittsburgh Southern Railway | | B&O | 1881 | 1884 | N/A | Sold at foreclosure; no property in West Virginia |
| Pittsburg and Steubenville Railroad | | PRR | 1856 | 1867 | Pan Handle Railway |
| Pittsburgh and West Virginia Railroad | | | 1967 | | | Still exists as a lessor of the Wheeling and Lake Erie Railway |
| Pittsburgh and West Virginia Railway | P&WV | P&WV | 1916 | 1967 | Pittsburgh and West Virginia Railroad |
| Pittsburgh, Wheeling and Kentucky Railroad | | PRR | 1871 | 1917 | Pittsburgh, Cincinnati, Chicago and St. Louis Railroad |
| Pocahontas Railroad | | | 1901 | | N/A |
| Pocahontas, Coal River and Kanawha Railway | | C&O | 1899 | 1903 | Coal River and Western Railway |
| Point Pleasant Bridge Company | | NYC | 1886 | 1935 | Kanawha and Michigan Railway |
| Point Pleasant, Buckhannon and Tygart's Valley Railroad | | B&O | 1892 | 1912 | Baltimore and Ohio Railroad |
| Point Pleasant and Ohio River Railroad | | NYC | 1881 | 1885 | Kanawha and Michigan Railway, Point Pleasant Bridge Company |
| Pond Fork Railway | | C&O | 1915 | 1918 | Chesapeake and Ohio Railway |
| Pond Fork and Bald Knob Railroad | | C&O | 1919 | 1933 | Chesapeake and Ohio Railway |
| Porters Creek and Gauley Railroad | | | 1896 | 1904 | N/A |
| Potomac and Piedmont Coal and Railroad Company | | WM | 1866 | 1881 | West Virginia Central and Pittsburg Railway |
| Potomac Valley Railroad of West Virginia | | WM | 1890 | 1905 | Western Maryland Railroad |
| Powellton and Pocahontas Railway | | C&O | 1891 | 1902 | Chesapeake and Ohio Railway |
| Preston Railroad | | | 1897 | 1960 | N/A |
| Raleigh and Pocahontas Railroad | | | 1902 | | N/A |
| Raleigh and Southwestern Railway | | C&O | 1903 | 1910 | Chesapeake and Ohio Railway |
| Ravenswood, Spencer and Glenville Railway | | B&O | 1886 | 1912 | Baltimore and Ohio Railroad |
| Richwood and Gauley Railroad | | | 1911 | | N/A |
| Richmond, Toledo and Chicago Railroad | | NYC | 1881 | 1881 | Atlantic and Northwestern Railroad |
| Ripley and Mill Creek Valley Railroad | | B&O | 1886 | 1912 | Baltimore and Ohio Railroad |
| Roaring Creek and Belington Railroad | | B&O | 1893 | 1919 | Coal and Coke Railway |
| Roaring Creek and Charleston Railroad | | B&O | 1893 | 1904 | Roaring Creek and Belington Railroad |
| Rowlesburg and Southern Railroad | | | 1911 | 1950 | N/A |
| Scotts Run Railway | | MGA | 1923 | 1933 | Monongahela Railway |
| Sewell Valley Railroad | | C&O/ NYC | 1907 | 1931 | Nicholas, Fayette and Greenbrier Railroad |
| Shenandoah Valley Railroad | | N&W | 1870 | 1890 | Shenandoah Valley Railway |
| Shenandoah Valley Railway | | N&W | 1890 | 1890 | Norfolk and Western Railroad |
| South Branch Railway | | B&O | 1871 | 1912 | Baltimore and Ohio Railroad |
| Strouds Creek and Muddlety Railroad | SCM | B&O | 1904 | 2000 | N/A | Most recently subleased to the Gauley River Railroad |
| Tug River and Kentucky Railroad | | N&W | 1913 | 1936 | Norfolk and Western Railway |
| Tunnelton, Kingwood and Fairchance Railroad | | | 1888 | 1899 | West Virginia Northern Railroad |
| Twin Mountain and Potomac Railroad | | | 1911 | 1919 | N/A |
| Valley River Railroad | | | 1907 | 1931 | N/A |
| Virginia–Maryland Coal Corporation | | B&O | 1907 | 1912 | Monongahela River Railroad |
| Virginian Railway | VGN | N&W | 1907 | 1959 | Norfolk and Western Railway |
| Virginian and Western Railway | | N&W | 1922 | 1936 | Virginian Railway |
| Virginian–Wyoming Railway | | N&W | 1919 | 1922 | Virginian and Western Railway |
| Wabash Pittsburgh Terminal Railway | WPT | P&WV | 1904 | 1916 | Pittsburgh and West Virginia Railway |
| Walkersville and Ireland Railroad | | | 1907 | 1924 | Walkersville and Southern Railroad |
| Walkersville and Southern Railroad | | | 1924 | 1930 | N/A |
| Wardensville Railroad | | | 1929 | 1929 | Winchester and Wardensville Railroad |
| Waynesburg Southern Railroad | WAS | MGA | 1966 | 1976 | Consolidated Rail Corporation |
| West Virginia Railroad | | B&O | 1886 | 1897 | Morgantown and Kingwood Railroad |
| West Virginia Central and Pittsburg Railway | | WM | 1881 | 1905 | Western Maryland Railroad |
| West Virginia and Ironton Railroad | | N&W | 1888 | 1890 | Norfolk and Western Railroad |
| West Virginia Midland Railroad | | | 1905 | 1924 | West Virginia Midland Railway |
| West Virginia Midland Railway | | | 1924 | 1931 | N/A |
| West Virginia Midland Railway | | B&O | 1883 | 1886 | Fairmont, Morgantown and Pittsburg Railroad, West Virginia Railroad |
| West Virginia Northern Railroad | WVN | | 1899 | 1991 | N/A | Continued as a tourist railroad until 1999 |
| West Virginia and Pittsburg Railroad | | B&O | 1890 | 1912 | Baltimore and Ohio Railroad |
| West Virginia Short Line Railroad | | B&O | 1895 | 1912 | Baltimore and Ohio Railroad |
| West Virginia South Western Railroad | | N&W | 1902 | 1909 | Norfolk and Western Railway |
| West Virginia and Southern Railroad | | | 1897 | | N/A |
| WV Southern Railway | WVSR | | 2003 | 2005 | R.J. Corman Railroad/West Virginia Line |
| West Virginia Southern Railway | | | 1895 | 1897 | West Virginia and Southern Railroad |
| Western Maryland Railroad | | WM | 1905 | 1909 | Western Maryland Railway |
| Western Maryland Railway | WM | WM | 1909 | 1989 | CSX Transportation |
| Weston and Buckhannon Railroad | | B&O | 1882 | 1890 | Clarksburg, Weston and Midland Railroad |
| Weston and Centreville Railroad | | B&O | 1882 | 1882 | Weston and Buckhannon Railroad |
| Weston and Elk River Railroad | | B&O | 1889 | 1889 | Clarksburg, Weston and Midland Railroad |
| Weston and West Fork Railroad | | B&O | 1875 | 1889 | Clarksburg, Weston and Midland Railroad |
| Wheeling Bridge and Terminal Railway | | PRR | 1889 | 1900 | Wheeling Terminal Railway |
| Wheeling and Harrisburg Railway | | PRR | 1882 | 1889 | Wheeling Bridge and Terminal Railway |
| Wheeling and Lake Erie Railroad | | NKP | 1899 | 1916 | Wheeling and Lake Erie Railway |
| Wheeling and Lake Erie Railway | | NKP | 1890 | 1899 | Wheeling and Lake Erie Railroad |
| Wheeling and Lake Erie Railway | W&LE, WLE | NKP | 1916 | 1949 | New York, Chicago and St. Louis Railroad |
| Wheeling, Parkersburg and Charleston Railway | | B&O | 1881 | 1882 | Ohio River Railroad |
| Wheeling, Pittsburgh and Baltimore Railroad | | B&O | 1872 | | |
| Wheeling Terminal Railway | | PRR | 1900 | 1954 | Penndel Company |
| White Oak Railway | | C&O, N&W | 1905 | 1917 | Chesapeake and Ohio Railway, Virginian Railway |
| White Sulphur and Huntersville Railroad | | | 1912 | | N/A |
| Williamson and Pond Creek Railroad | | N&W | 1912 | 1936 | Norfolk and Western Railway |
| Williamsport, Nessle and Martinsburg Railway | | WM | 1913 | 1937 | Western Maryland Railway |
| Winchester and Potomac Railroad | | B&O | 1831 | 1988 | CSX Transportation |
| Winchester and Wardensville Railroad | | | 1929 | 1934 | N/A |
| Winchester and Western Railroad | | | 1916 | 1930 | Winchester and Wardensville Railroad |
| Winding Gulf Railroad | | | 1906 | | N/A |
| Winifrede Railroad | WNFR | | 1881 | 1987 | Kanawha Rail Corporation |
